Agilent 2100 tapestation system

Except seedling stems, the tissues of different types of moso bamboo growing culms were collected from Xuancheng (E119°41′; N30°89′), Anhui Province. Bamboo seeds were germinated and grown in phytotron. Seven represented tissues including winter bamboo shoot (B1), 1.5 m height spring bamboo shoot (B2), lateral buds (L), 0.5 m long rhizomes (R), 0.3 m long outward rhizomes (OR), 1.5 cm height seedling stem (S1), and 4.5 cm height seedling stem (S2) were selected. B2, OR, S1, and R were sampled when the culms reached about one tenth of the final length. For each culm sample, the top fifteen percent length of the culms were defined as the culm tips and used for further transcriptome sequencing (Figure 1).
Total RNA from each sample was extracted from each culm samples using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). RNA with high purity and integrity which examined by Agilent 2100 TapeStation system (Agilent Technologies, Santa Clara, CA, USA) and Nano Photometer spectrophotometer (IMPLEN, Munich, Germany) was further used for the library construction.

Genomic DNA was isolated from hair or peripheral blood using Gentra Puregene Blood Kit (Qiagen) following the manufacturer’s protocol. The DNA was checked for quality and quantity with Nanodrop 2000 spectrophotometer (Thermo Scientific).
The IDs and sequence coordinates of 4 partially overlapping genomic clones spanning the deletion in ECA29 and a control clone outside the deletion (Table 1) were retrieved from NCBI (https://www.ncbi.nlm.nih.gov/genome/?term=horse). The clones were picked from the CHORI 241 BAC library (https://bacpacresources.org/). High molecular weight BAC DNA was isolated with High Pure Plasmid Isolation Kit (Roche Diagnostics) per manufacturer instructions and checked for molecular weight, quality and quantity with Agilent 2100 TapeStation system.