Ovsaho

The human ovarian cancer cell lines: MCAS and OVSAHO (JCRB cell bank, Osaka, Japan, catalog no. JCRB0240 and no. JCRB1046 respectively) were kindly provided by Prof. Aikou Okamoto (Jikei University School of Medicine, Japan), in 2016. The cisplatin sensitive A2780 (The European Collection of Authenticated Cell, ECACC catalog no. 93112519) and cisplatin-resistant A2780-cis (ECACC catalog no. 93112517) cell lines were a generous gift from Dr. Benjamin Tsang (University of Ottawa, Canada), in 2018. The transformed normal epithelial ovarian cell line HOSE6–3 (RRID: CVCL_7673), established by Prof. GSW Tsao (School of Biomedical Sciences, The University of Hong Kong), was kindly provided by his laboratory in 2018. To avoid contaminations, our cell-culture laboratories, including hoods and incubators, are systemically fumigated every year, and any new cells are tested upon arrival, for the presence of mycoplasma using the “Mycoplasma Detection Kit” (Lonza, Catalog #: LT07–118). None of the cells used for this study were tested positive.
MCAS, A2780, A2780 cis and HOSE6–3 cells were propagated in DMEM (Gibco, NY, USA), while OVSAHO was cultured in RPMI-1640 media supplemented with 10% FBS (Gibco, NY, USA) and 1% penicillin-streptomycin antibiotic (Gibco, NY, USA) in a humidified incubator at 37 °C and 5% CO₂.

RMG-I [32] (link), OVMANA [33] (link), OVTOKO [34] (link), OVISE [34] (link), OVSAHO [33] (link), and OVKATE [33] (link) cells were obtained from the Japanese Collection of Research Bioresources Cell Bank. OVCAR-3 cells [35] (link) were obtained from the RIKEN BioResource Center. PEO1, PEO4, PEO14, PEO23 [17] (link), and A2780 [36] (link) cells were purchased from the European Collection of Cell Cultures, and TOV21G [37] (link) and ES-2 [38] (link) cells were purchased from the American Type Culture Collection. RMG-I cells were cultured in Ham’s F12 medium supplemented with 10% fetal bovine serum, ES-2 cells in McCoy’s 5a medium supplemented with 10% fetal bovine serum, TOV21G cells in MCDB105/Medium 199 (1∶1) supplemented with 10% fetal bovine serum, and HeLa cells in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum. All other cell lines (A2780, OVCAR-3, OVMANA, OVTOKO, OVISE, OVSAHO, OVKATE, PEO1, PEO4, PEO14, and PEO23) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1 mM sodium pyruvate (Gibco, Grand Island, NY, USA).