Log In Sign up
The largest database of trusted experimental protocols

Streptavidin hrp complex

Manufactured by Agilent Technologies
Visit Supplier
Sourced in Denmark, United Kingdom

The Streptavidin-HRP complex is a laboratory reagent used in various bioanalytical and diagnostic applications. Streptavidin, a protein derived from the bacterium Streptomyces, is covalently linked to the enzyme horseradish peroxidase (HRP). This complex is widely employed as a detection agent in techniques such as enzyme-linked immunosorbent assays (ELISAs), Western blots, and other bioassays that rely on the high-affinity interaction between streptavidin and biotin.

Automatically generated - may contain errors

Lab products found in correlation

Novared kit, by Vector Laboratories (1 mentions) Cd31 clone jc70a, by Agilent Technologies (1 mentions) Tween 20, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by Serva Electrophoresis (1 mentions) H2so4, by Carl Roth (1 mentions) Rat igg2a, by R&D Systems (1 mentions) Bovine serum albumin (bsa), by AppliChem (1 mentions) Biotin labelled secondary antibodies, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Streptavidin-biotin-horseradish peroxidase (HRP) complex HRP-conjugated streptavidin-biotin complex Streptavidin-HRP

3 protocols using streptavidin hrp complex

1

Immunohistochemical Analysis of Vascular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Individual cross-sections were incubated with antibodies against aquaporin-1 (clone 1/A5F6, Abcam, Cambridge, UK), ICAM-1 (clone My13, Zymed, South San Francisco, CA), CD31 (clone JC70A, Dako, Glostrup, Denmark), monocyte/macrophage (clone HAM56, Dako), actin (smooth muscle)(clone 1A4, Dako). All antibodies were diluted in TBS containing 1% weight/volume (w/v) bovine serum albumin (BSA), (Sigma-Aldrich, Zwijndrecht, the Netherlands) and 0.01% (w/v) Tween-20 (Sigma-Aldrich). The incubations with secondary biotin-conjugated antibodies (Dako) were followed by amplification with a streptavidin-HRP complex (Dako), and a peroxidase-substrate staining (Nova Red kit, Vector Labs, Burlingame, CA).
Fontijn R.D., Volger O.L., van der Pouw-Kraan T.C., Doddaballapur A., Leyen T., Baggen J.M., Boon R.A, & Horrevoets A.J. (2015). Expression of Nitric Oxide-Transporting Aquaporin-1 Is Controlled by KLF2 and Marks Non-Activated Endothelium In Vivo. PLoS ONE, 10(12), e0145777.
+ Open protocol
+ Expand
2

Binding Assay of 8H7-mAb and CyPA

Check if the same lab product or an alternative is used in the 5 most similar protocols
The binding between 8H7-mAb and CyPA was tested in a modified enzyme-linked immunoabsorbent assay (ELISA)(28 (link)). A 96 well plate was coated overnight with 200 nmol/L of CyPA and blocked overnight with 1% BSA (Applichem, St. Louis, MO, USA). On the next day 8H7-mAb or ratIgG2a (R&D Systems, Minneapolis, MN, USA) were added in concentrations of 0, 10, 50, 100 μg/ml and incubated for 3 hours at room temperature. For detection of the primary 8H7-mAb, a biotinylated secondary antibody (rabbit anti-rat, Dako, Glostrup, Denmark) was added followed by incubation with a streptavidin-HRP complex (Dako). Binding was detected with 3,3′,5,5′-tetramethylbenzidine (Serva, Heidelberg, Germany) and the reaction was stopped with 1 M H2SO4 (Carl Roth GmbH, Karlsruhe, Germany). The reaction was measured in an ELISA plate reader at 450 nm with a reference value of 570 nm.
von Ungern-Sternberg S.N., Vogel S., Walker-Allgaier B., Geue S., Maurer A., Wild A.M., Münzer P., Chatterjee M., Heinzmann D., Kremmer E., Borst O., Loughran P., Zernecke A., Neal M.D., Billiar T.R., Gawaz M, & Seizer P. (2017). Extracellular cyclophilin A augments platelet-dependent thrombosis and thrombo-inflammation. Thrombosis and haemostasis, 117(11), 2063-2078.
+ Open protocol
+ Expand
3

Detecting CYP450 Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Specific anti-peptide antibodies raised against individual CYP450 enzymes and the corresponding pre-immune sera have been previously described (Edwards et al, 1998 (link); Kapucuoglu et al, 2003 (link)). These antibodies have previously been used to detect human CYP450 protein expression (Piipari et al, 2000 (link), Rodriguez-Antona et al, 2002 (link)). Biotin labelled secondary antibodies and streptavidin/HRP complex were obtained from DAKO Ltd (Ely, UK). All other chemicals were supplied by either Sigma-Aldrich or Merck (Nottingham, UK) unless otherwise specified.
Patel S.A., Bhambra U., Charalambous M.P., David R.M., Edwards R.J., Lightfoot T., Boobis A.R, & Gooderham N.J. (2014). Interleukin-6 mediated upregulation of CYP1B1 and CYP2E1 in colorectal cancer involves DNA methylation, miR27b and STAT3. British Journal of Cancer, 111(12), 2287-2296.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!