Lrrk2in1

Manufactured by Cayman Chemical

Sourced in United States

LRRK2in1 is a laboratory equipment product designed to facilitate the study of the LRRK2 protein. It is a tool for researchers to investigate the structure and function of this protein, which is associated with certain neurodegenerative disorders. The product provides a controlled environment and specialized components to support the analysis of LRRK2 in a research setting.

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Lab products found in correlation

2 protocols using lrrk2in1

Compound Screening for Drug Discovery

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Carbonyl cyanide m-chlorophenyl hydrazine (CCCP) and okadaic acid were from Sigma (Sigma-Aldrich, St. Louis, MO, USA). AZD1080, CHIR98014, cyclosporin A, GNE7915, LRRK2in1, oligomycin, rotenone, SB216763, sorafenib, staurosporine, and valinomycin were from Cayman Chemical Company (Ann Arbor, MI, USA). Tipranavir (#11285) was obtained through the NIH’s HIV Reagent Program, which is supported by National Institute of Allergy and Infectious Diseases. 10 mM stock solutions in dimethyl sulfoxide (DMSO) were prepared for all compounds with the exception of staurosporine (1mg/ml in ethyl acetate) and stored at −20°C until further use. Working dilutions were prepared fresh for each experiment.

, & Alavi M.V. (2021). Tau phosphorylation and OPA1 proteolysis are unrelated events: Implications for Alzheimer’s Disease.. Biochimica et biophysica acta. Molecular cell research, 1868(12), 119116.

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Comprehensive Experimental Toolkit for Cell Biology

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Reagents and kits were obtained from the indicated suppliers: ADP‐Glo Assay kit (Promega, Madison, WI, USA), alkaline phosphatase (CIAP) (TAKARA, Kusatsu, Shiga, Japan), ATP Determination Assay kit (Molecular Probes, Eugene, OR, USA), 2APB (Sigma‐Aldrich), bafilomycin A1 (Sigma‐Aldrich), BD Matchmaker Pretransformed cDNA Library (Clontech Laboratories), bradykinin (Sigma‐Aldrich), Citrate Synthase Assay kit (Sigma‐Aldrich), Duolink in situ assay (Sigma‐Aldrich), E1 enzyme (Abcam, Cambridge, UK), anti‐FLAG Sepharose (Sigma‐Aldrich), and FLAG peptides (Sigma‐Aldrich), Lipofectamine 3000 (Thermo Fisher, Waltham, MA USA), LRRK2‐IN‐1 (Cayman Chemical, Ann Arbor, MI, USA), MISSION siRNA mouse kinase panel library (Sigma‐Aldrich), Mitotracker (Molecular Probe), Ni‐NTA beads (Qiagen, Germantown, MD, USA), Ru360 (Sigma‐Aldrich), and tunicamycin (Sigma‐Aldrich), UbcH7 (Abcam), and Vybrant MTT Cell Proliferation Assay Kit (Thermo Fisher).

Toyofuku T., Okamoto Y., Ishikawa T., Sasawatari S, & Kumanogoh A. (2019). LRRK2 regulates endoplasmic reticulum–mitochondrial tethering through the PERK‐mediated ubiquitination pathway. The EMBO Journal, 39(2), e100875.

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