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3 methylcholanthrene mca

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Sourced in China, France

3-methylcholanthrene (MCA) is a synthetic chemical compound. It is used as a laboratory reagent for research purposes.

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Lab products found in correlation

Ammonium bicarbonate, by Sinopharm (1 mentions) Iodized oil, by Tokyo Chemical Industry (1 mentions) Diethylnitrosamine den, by Tokyo Chemical Industry (1 mentions) Anti bcl 2 and anti bax antibodies, by Cell Signaling Technology (1 mentions) Diphtheria toxin, by Merck Group (1 mentions) Mouse igg1 isotype control, by BioXCell (1 mentions) Rat anti mouse pd l1, by BioXCell (1 mentions) Rat igg2b isotype control, by BioXCell (1 mentions) Rat igg1 isotype control, by BioXCell (1 mentions) Suberoylanilide hydroxamic acid, by Merck Group (1 mentions) 5 aza 2 deoxycytidine, by Merck Group (1 mentions)

6 protocols using 3 methylcholanthrene mca

1

Carcinogen-Induced Mouse Sarcoma Model

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To mimic the clinical presentation and progression of human sarcomas with gradual tumor development and metastasis in the setting of an intact immune system, a carcinogen-induced GEMM of sarcoma (Lee CL, Mowery YM, Daniel AR, et all, submitted) was chosen for the animal studies of the co-clinical trial. Primary sarcomas (p53/MCA model) are generated by intramuscular delivery into the gastrocnemius of adenovirus expressing Cre recombinase (Adeno-Cre; Gene Transfer Vector Core, University of Iowa) into p53fl/fl mice followed by intramuscular injection of 0.3 mg 3-methylcholanthrene (MCA; Sigma-Aldrich, Saint Louis, MO) at the same site. Tumors develop approximately 8–12 weeks after induction. Imaging studies were initiated when tumors were palpable (>100 mm3), as well as at various stages of disease progression.
Blocker S.J., Mowery Y.M., Holbrook M.D., Qi Y., Kirsch D.G., Johnson G.A, & Badea C.T. (2019). Bridging the translational gap: Implementation of multimodal small animal imaging strategies for tumor burden assessment in a co-clinical trial. PLoS ONE, 14(4), e0207555.
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2

Oridonin-loaded PLGA Nanoparticles for Liver Cancer

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Oridonin was obtained from the Shaanxi Huike Botanical Development Co., Ltd., Shaanxi, China. Poly(lactic-co-glycolic)acid (PLGA, lactide/glycolide, 50:50, mol/mol, MW 10 kDa) was produced by Jinan Daigang Biomaterial Co., Ltd., Shandong, China. Gemcitabine, used as a positive control drug, was purchased from Hansoh Pharmaceutical Co., Ltd., Jiangsu, China. Polyvinyl alcohol (PVA, 87%–89% alcoholysis, MW 75000 Da) was purchased from the Aladdin Industrial Corporation, Shanghai, China, and ammonium bicarbonate was purchased from the Sinopharm Chemical Reagent Co., Ltd., Beijing, China. Cy7 was purchased from Fanbo Biochemicals Co., Ltd., Beijing, China. 3-Methyl cholanthrene (MCA, Sigma, USA), diethyl nitrosamine (DEN, Tokyo Chemical Industry, Japan), and iodized oil (Guerbet, French) were used. Anti-BCL-2 and anti-BAX antibodies were from the Cell Signaling Technology Inc. (Danvers, USA). All other chemicals and solvents were of analytical grade or high performance liquid chromatographic (HPLC) grade.
Zhu L., Li M., Liu X., Du L, & Jin Y. (2016). Inhalable oridonin-loaded poly(lactic-co-glycolic)acid large porous microparticles for in situ treatment of primary non-small cell lung cancer. Acta Pharmaceutica Sinica. B, 7(1), 80-90.
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3

Subcutaneous 3-Methylcholanthrene Tumour Induction

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Mice were anaesthetized and injected subcutaneously (in the hind leg) with 400 μg of 3-methylcholanthrene (MCA; Sigma-Aldrich, St Louis, MO) in 100 μl of olive oil. Tumours occurred between 80 and 150 days after injection. Tumour-bearing mice were killed before the tumours reached 1·5 cm in diameter.
Ondondo B., Colbeck E., Jones E., Smart K., Lauder S.N., Hindley J., Godkin A., Moser B., Ager A, & Gallimore A. (2015). A distinct chemokine axis does not account for enrichment of Foxp3+ CD4+ T cells in carcinogen-induced fibrosarcomas. Immunology, 145(1), 94-104.
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4

Inducing Tumors and Depleting Immune Cells in Mice

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Anesthetized mice (8–15 weeks old) were injected subcutaneously with 400 μg of 3-methylcholanthrene (MCA; Sigma-Aldrich) in 100 μl olive oil to induce tumors as previously described (12 (link)). Mice were monitored for tumor development weekly for up to 18 months. Diphtheria toxin (DT; Sigma-Aldrich) in 100 μl PBS was administered by intraperitoneal (i.p.) injection (5ng/g body weight to deplete Tregs; 8ng/g body weight to deplete Tregs and CD11c+ cells) every other day after palpable tumor development. Once tumors became palpable, they were measured using calipers every other day (tumor width, tumor height, tumor leg diameter and non-tumor leg diameter), and tumor growth rate (k, days−1) was calculated using the difference between tumor and nontumor leg diameters by the following equation: Y= Y0 × exp (k × X). Mice were sacrificed before tumors reached 1.5cm in diameter or if tumors caused apparent discomfort (irritation or decreased mobility).
Colbeck E.J., Jones E., Hindley J.P., Smart K., Schulz R., Browne M., Cutting S., Williams A., Parry L., Godkin A., Ware C.F., Ager A, & Gallimore A. (2017). Treg Depletion Licenses T Cell–Driven HEV Neogenesis and Promotes Tumor Destruction. Cancer immunology research, 5(11), 1005-1015.
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5

Characterization of Tumor Immune Infiltrates

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PE-conjugated anti-mouse CD274 (PD-L1, B7-H1) and other additional antibodies for flow cytometry were from eBioscience as described. Mouse CXCL12 (clone: 79014) neutralizing antibody was from R&D systems. Rat anti-mouse IFN-γ (clone: XMG1.2), rat IgG1 isotype control (clone: HRPN), rat anti-mouse CD25 (clone: PC-61.5.3), rat anti-mouse PD-L1 (clone: 10F.9G2), rat IgG2b isotype control (clone: LTF-2) and mouse IgG1 isotype control (clone: MOPC-21) antibodies were from Bioxcell. 3-Methylcholanthrene (MCA) was from Sigma-Aldrich. MCA-205 cell line was kindly provided by Dr. Tomasz Zal (MD Anderson Cancer Center, TX).
Zou Q., Jin J., Xiao Y., Zhou X., Hu H., Cheng X., Kazimi N., Ullrich S.E, & Sun S.C. (2015). T cell intrinsic USP15 deficiency promotes excessive IFN-γ production and an immunosuppressive tumor microenvironment in MCA-induced fibrosarcoma. Cell reports, 13(11), 2470-2479.
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6

Silica Nanoparticle Characterization Protocol

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Min-U-Sil® 5 was obtained from US Silica Co. (Berkeley Springs, WV, USA), whereas NM-203 was obtained from the Joint Research Centre nanomaterials repository (Ispra, Italy).
Particles were dispersed in sterile water and sonicated for 5 min at 10 % amplitude using a Branson Sonifier S-450D equipped with a cup-horn device (Branson Ultrasonics Crop, Danbury, CT). The physical and chemical characteristics of the silica samples are presented in Table S1 (Elias et al., 2006 , Rasmussen et al., 2013 ). 3-methylcholanthrene (MCA, Sigma-Aldrich, Saint Quentin Fallavier, France, ref# 213942), 12-O-tetradecanoylphorbol-13acetate (TPA, Sigma-Aldrich, ref# 1585) , suberoylanilide hydroxamic acid (Sigma-Aldrich, ref# SML0061) and 5-aza-2'-deoxycytidine (Sigma-Aldrich, ref# A3656) were dissolved in DMSO.
Seidel C., Kirsch A., Fontana C., Visvikis A., Remy A., Gaté L., Darne C, & Guichard Y. (2017). Epigenetic changes in the early stage of silica-induced cell transformation. Nanotoxicology, 11(7).
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