Cellulose acetate

Manufactured by Avantor

Sourced in United States

Cellulose acetate is a type of laboratory equipment used for various applications. It is a synthetic polymer derived from cellulose and acetic acid. Cellulose acetate has a range of properties that make it useful in laboratory settings, such as its transparency, flexibility, and chemical resistance.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Virapower lentiviral packaging mix, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin fraction 5, by Merck Group (1 mentions) Avance 600 mhz, by Bruker (1 mentions) Sodium tripolyphosphate, by Thermo Fisher Scientific (1 mentions) Acetone, by Avantor (1 mentions) L cysteine, by Avantor (1 mentions)

9 protocols using cellulose acetate

Optimized Multiplexed CRISPR Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Fred Hutchinson Co-operative Center for Excellence in Hematology Vector Production core produced all virus for the multiplexed enhancer-gene pair screening experiments. For the singleton CRISPRi recapitulation, virus was made in-house by co-transfecting (Lipofectamine 3000, ThermoFisher, L300015) HEK293Ts with the small pools of CRISPRi-optimized CROP-seq with the ViraPower Lentiviral Packaging Mix (ThermoFisher). After 3 days, supernatant was syringe filtered with a 0.45 uM filter (cellulose acetate, VWR) to prepare virus for transduction.
Cells were transduced (8 μg/mL polybrene) with varying titers and amounts of virus to achieve differing MOI. 400,000 and ~2.5 million original cells were transduced for the pilot and at-scale experiments, respectively. At 24 hours post-transduction, cells were spun and resuspended with virus- and polybrene- free media. At a total 48 hours post-transduction, 2 μg/mL puromycin was added to the culture, and changed to 1 μg/mL puromycin at the next passage for maintenance. A total of 10 days post transduction, cells were collected for scRNA-seq or bulkRNA-seq.

Gasperini M., Hill A.J., McFaline-Figueroa J.L., Martin B., Kim S., Zhang M.D., Jackson D., Leith A., Schreiber J., Noble W.S., Trapnell C., Ahituv N, & Shendure J. (2019). A Genome-wide Framework for Mapping Gene Regulation via Cellular Genetic Screens. Cell, 176(1-2), 377-390.e19.

+ Open protocol

+ Expand

Osteogenic Differentiation of hMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

In vitro cell studies were performed with human mesenchymal stem cells (hMSCs) in passage 5-7. Growth media (GM) was composed of Dulbecco’s Modified Essential Medium (DMEM) supplemented with 10% fetal bovine serum and 100 units/mL of penicillin-streptomycin. Osteogenic media (OG) was composed of growth media supplemented with 0.2 mM ascorbic acid, 50 nM dexamethasone, and 10 mM β-glycerophosphate disodium. Solid samples were submerged in 70% ethanol for 1 hour and sterilized by UV irradiation for 1 hour on each surface. For liquid samples, solutions were sterilized by filtering through a sterile syringe filter (0.2 μm cellulose acetate, VWR, PA). For all cell studies, cells were kept in a humidified incubator at 37 °C with 5% CO₂. Both GM and OM were replaced every other day.

He Y., Li Q., Ma C., Xie D., Li L., Zhao Y., Shan D., Chomos S.K., Tierney J.W., Sun L., Lu D., Gui L, & Yang J. (2019). Development of Osteopromotive Poly (octamethylene citrate glycerophosphate) for Enhanced Bone Regeneration. Acta biomaterialia, 93, 180-191.

+ Open protocol

+ Expand

Prebiotic and Probiotic Evaluation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two commercially available prebiotic products were used in this study. Inulin derived from chicory (90%; DP 2-60; Orafti^®GR, Beneo GmbH, Oreye, Belgium) and fructooligosaccharides (FOS) produced by partial enzymatic hydrolysis from chicory inulin (93–97% β-(2,1)-linkage oligofructose; DP 2-8; Orafti^®P95, Beneo GmbH, Oreye, Belgium) were diluted in PBS to a concentration of 20 mg/mL and sterile-filtered (0.2 µm cellulose acetate; VWR international, Radnor, PA, USA). Enterococcus faecium DSM 7134 (10¹⁰ cfu/g, Bonvital Pellets, Lactosan GmbH & Co.KG, Kapfenberg, Germany) and Bacillus coagulans DSM 32016 (2.5 × 10⁹ cfu/g, TechnoSpore^®, Biochem, Lohne, Germany) served as probiotic products. Both probiotics are licensed in the EU as gut flora stabilizers in chickens according to council regulation (EC) No. 1831/2003. The final concentration on microplates was 2 mg/mL for prebiotic products and 10⁷ cfu/mL for probiotic products. The final antibiotic mixture (see Section 2.4) was also tested on the probiotic products to guarantee the absence of growth of probiotic bacteria in the assay.

Fuhrmann L., Vahjen W., Zentek J., Günther R, & Saliu E.M. (2022). The Impact of Pre- and Probiotic Product Combinations on Ex vivo Growth of Avian Pathogenic Escherichia coli and Salmonella Enteritidis. Microorganisms, 10(1), 121.

+ Open protocol

+ Expand

Preparation of Bovine Serum Albumin Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bovine serum albumin, fraction V (EMD Millipore) was dissolved in 1× phosphate buffered saline (PBS) to make 10 mg/ml solutions. Serial dilutions were made from the 10 mg/ml BSA in 1× PBS stock solution to make a series of concentrations from 0.625 mg/ml to 5 mg/ml. BSA solutions and buffers were filtered with a 0.2 μm cellulose acetate (VWR) or polyethersulfonate (EMD Millipore) filter to remove dust and impurities prior to scattering experiments.

Ye D., Le T.P., Kuei B., Zhu C., Zwart P.H., Wang C., Gomez E.D, & Gomez E.W. (2018). Resonant Soft X-Ray Scattering Provides Protein Structure with Chemical Specificity. Structure (London, England : 1993), 26(11), 1513-1521.e3.

+ Open protocol

+ Expand

Quantification of WTEA Phytochemicals by NMR

Check if the same lab product or an alternative is used in the 5 most similar protocols

WTEA from a commercial brand was prepared daily, according to the manufacturer’s instructions. Briefly, samples were subjected to infusion (1 g/100 mL of distilled boiling water) during 3 min. The infusion was filtered through a 0.2 μm sterile syringe filter of cellulose acetate (VWR, PA, USA). For determination of WTEA composition, 5 different extracts were analyzed by Proton Nuclear Magnetic Resonance (¹H-NMR) as previously described [5 (link),6 (link),7 (link),8 (link)]. In brief, ¹H-NMR spectra of WTEA samples were acquired at 14.1 T, 25 °C, using a Bruker Avance 600 MHz spectrometer (Bruker BioSpin, Rheinstetten, Germany). Sodium fumarate (final concentration of 1 mM) was used as an internal reference (6.50 ppm) to quantify the following phytochemicals (multiplet, ppm): L-theanine (triplet, 1.08); lactate (doublet, 1.33); alanine (doublet, 1.45); (-)-epigallocatechin-3-gallate (EGCG) (doublet, 2.7); caffeine (singlet, 3.29); H1-α-glucose (doublet, 5.22); sucrose (doublet, 5.4); (-)-epigallocatechin (EGC) (singlet, 6.6); (-)-epicatechin (EC) (singlet, 7.0). The relative areas of 1 H NMR resonances were quantified using the curve-fitting routine supplied with the NUTSproTM NMR spectral analysis program (Acorn, NMR Inc., Fremont, CA, USA).

Silveira A.C., Rato L., Oliveira P.F., Alves M.G, & Silva B.M. (2021). White Tea Intake Abrogates Markers of Streptozotocin-Induced Prediabetes Oxidative Stress in Rat Lungs’. Molecules, 26(13), 3894.

+ Open protocol

+ Expand

Preparation of SiNc-based Polymeric Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

SiNc-based polymeric nanoparticles were prepared via a solvent evaporation method 35 , 37 . In general, PEG-PCL (20 mg) and the desired amount of SiNc (0.1, 0.6 (always-ON), or 1.2 (activatable) mg) were dissolved and mixed in tetrahydrofuran (THF) (2 mL). Under constant stirring, saline (2 mL) was then added to the vial. THF was evaporated using a rotary evaporator with a water bath temperature of 40 °C and a rotation speed of 100 rpm. The evaporation followed a three-segment cycle: first at 400 mbar for 7 min, then 320 mbar for 7 min, and finally 200 mbar for 6 min. The final volume of the nanoparticle solution was adjusted to 2 mL with saline and centrifuged at 10,062 rcf for 5 min. The supernatant was then filtered through a 0.2 µm filter (cellulose acetate, VWR International, Radnor, PA) to collect dispersed nanoparticles with desired SiNc loading amounts (0.05, 0.3 (always-ON), and 0.6 (activatable) mg/mL; loading efficiency was calculated by SiNc (mg) / polymer (mg) × 100% = 0.5%, 3%, and 6%, respectively). The preparation and characterization of always-ON SiNc-PNP (0.3 mg/mL loading) and IR780-PNP was reported earlier by our group 30 (link), 35 .

Li X., Schumann C., Albarqi H.A., Lee C.J., Alani A.W., Bracha S., Milovancev M., Taratula O, & Taratula O. (2018). A Tumor-Activatable Theranostic Nanomedicine Platform for NIR Fluorescence-Guided Surgery and Combinatorial Phototherapy. Theranostics, 8(3), 767-784.

+ Open protocol

+ Expand

Chitosan Nanoparticle Synthesis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellulose acetate (MW ∼100,000 Da; acetyl content ∼39.7 wt%) was purchased from VWR International (VWR international, Radnor, PA, USA). Low-molecular-weight chitosan (100–150 kDA, DDA ≈ 85%) and sodium tripolyphosphate (TPP) were purchased from Thermo Fisher Scientific (Thermo Fisher Scientific Inc., Waltham, MA, USA). The enzyme-linked immunosorbent assay (ELISA) kits used throughout the present work were purchased from Shanghai X-Y Biotechnology Co.

Elbhnsawi N.A., Elwakil B.H., Hassanin A.H., Shehata N., Elshewemi S.S., Hagar M, & Olama Z.A. (2023). Nano-Chitosan/Eucalyptus Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities. Membranes, 13(6), 604.

+ Open protocol

+ Expand

Synthesis of Cadmium Selenide Nanoparticles

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellulose acetate (MW ∼100,000 Da; acetyl content ~39.7 wt%) and acetone were purchased from VWR International (Radnor, PA, USA).
Based on the slow reaction between Cd²⁺ and Se²⁻ ions in an aqueous basic bath with pH > 10, the CdSe nanoparticles were synthesized using a chemical bath deposition process. Cadmium sulfate (CdSO₄) and sodium selenosulfite (Na₂SSeO₃) were used as the sources for Cd²⁺ and Se²⁻, respectively. Na₂SSeO₃ was prepared by dissolving elemental Se in the form of fine powder in an aqueous solution of sodium sulfite heated to 60 °C. The solution was stirred well until the Se was completely dissolved. The pH of the solution was adjusted by adding excess NaOH. CdSe was formed in 2 h at a temperature of 70 °C. The obtained CdSe powder was washed using deionized water, centrifuged repeatedly, and subsequentially dried in a vacuum oven.
Because CdSe is considered toxic to human cells, it was handled with the utmost care during this study [18 (link)]. CdSe toxicity has been mainly attributed to the release of Cd²⁺ ions from the CdSe to surrounding cells [18 (link)]. The method of exposure by which this diffusion occurs highly alters how significantly the affected cells react to CdSe [18 (link)]. Consequently, equipment in contact with CdSe was cleaned thoroughly, waste was properly disposed of, and the CdSe NPs were properly labeled and stored before and after use.

Angel N., Vijayaraghavan S.N., Yan F, & Kong L. (2020). Electrospun Cadmium Selenide Nanoparticles-Loaded Cellulose Acetate Fibers for Solar Thermal Application. Nanomaterials, 10(7), 1329.

+ Open protocol

+ Expand

Prebiotic and Probiotic Evaluation for Sows and Piglets

Check if the same lab product or an alternative is used in the 5 most similar protocols

All prebiotic products are commercially available and are commonly used in sows and piglets. Fructo-oligosaccharides (FOS) produced by partial enzymatic hydrolysis from chicory inulin (93-97% β-(2,1)-linkage oligofructose; partial hydrolysis from chicory inulin; DP 2-8), mannanoligosaccharide (MOS) produced from Saccharomyces cerevisiae yeast cell walls (min. 24% mannans; 25% betaglucans, 25% protein) and inulin derived from chicory (90% inulin from chicory; DP 2-60) were diluted in phosphate buffered saline with 0,5 mg/ml L-cysteine (PBS, pH 7.0) and filter-sterilised (0.2 µm cellulose acetate; VWR international, Radnor, PA, USA) under anaerobic conditions. MOS and inulin were diluted to a final concentration of 20 mg/ml, while FOS was adjusted to 10 mg/ml final concentration.
All probiotic products are licensed in the EU as feed additives in sows and piglets. An Enterococcus faecium (1×10 10 cfu/g), a Saccharomyces cerevisiae (2.0×10 10 cfu/g) and a multistrain product containing Bacillus licheni formis and Bacillus subtilis (3.25×10 9 cfu/g) were diluted anaerobically from original powders in PBS. The final concentration for all probiotics was 10 7 cfu/ml in each ex vivo assay. Viable cell number per g product was determined before use.

Zeilinger K., Hellmich J., Zentek J, & Vahjen W. (2021). Novel ex vivo screening assay to preselect farm specific pre- and probiotics in pigs. Beneficial microbes, 12(6).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!