Recombinant human lbp protein

Manufactured by R&D Systems

Recombinant human LBP protein is a purified protein produced using recombinant DNA technology. LBP (Lipopolysaccharide Binding Protein) is a circulating protein that binds to lipopolysaccharides, which are components of the outer membrane of gram-negative bacteria.

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Lab products found in correlation

Alexa 594 nhs, by Thermo Fisher Scientific (2 mentions) Tnf α elisa kit, by R&D Systems (2 mentions) Pooled normal human plasma, by Innovative Research (1 mentions) Endotoxin free water, by GE Healthcare (1 mentions) Chromogenic lal endotoxin assay kit, by GenScript (1 mentions) Alexa 488 hydrazide, by Thermo Fisher Scientific (1 mentions)

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Recombinant proteins (13 citations)

2 protocols using recombinant human lbp protein

Characterization of LPS Interactions

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Highly pure rough LPS from E. coli K12 strain LCD25 was obtained from List biological labs. The FITC labelled LPS from E. coli 014 (Ra) and ³H/¹⁴C labelled LPS from Salmonella enterica sv. Typhimurium PR122 (Rc) were kind gift from Prof. Robert Munford (NIAID). Pooled normal human plasma was from Innovative Research. Endotoxin free water was from GE Healthcare Life Sciences. Fluorescein isothiocyanate (FITC), Alexa 488 hydrazide and Alexa 594 NHS were obtained from Molecular Probes. Purified apo-A1 from human plasma was obtained from Athens Research & Technology. The rabbit anti-apoA1 antibody was from Abcam. The TNF-α ELISA kit was from R&D. Chromogenic LAL Endotoxin Assay Kit was from Genscript. Recombinant human LBP protein was from R&D.

Yao Z., Mates J.M., Cheplowitz A.M., Hammer L.P., Maiseyeu A., Phillips G.S., Wewers M.D., Rajaram M.V., Robinson J.M., Anderson C.L, & Ganesan L.P. (2016). Blood-borne LPS is rapidly eliminated by liver sinusoidal endothelial cells via HDL. Journal of immunology (Baltimore, Md. : 1950), 197(6), 2390-2399.

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LPS Binding and Activation Assay

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Highly purified rough LPS from E. coli K12 strain LCD25 was from List Biological Labs (#314). The ³H/¹⁴C labeled LPS from Salmonella enterica sv. Typhimurium PR122 (Rc) was a kind gift from Prof. Robert Munford (NIAID). The normal plasma samples pooled from several individuals were from Innovative Research. The Alexa 488 hydrazide and Alexa 594 NHS were from Molecular Probes. The TNFα ELISA kit was from R&D. Recombinant human LBP protein was from R&D.

Cabral F., Al-Rahem M., Skaggs J., Thomas T.A., Kumar N., Wu Q., Fadda P., Yu L., Robinson J.M., Kim J., Pandey E., Sun X., Jarjour W.N., Rajaram M.V., Harris E.N, & Ganesan L.P. (2021). Stabilin receptors clear LPS and control systemic inflammation. iScience, 24(11), 103337.

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