Anti cyto c

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-Cyto c is a laboratory equipment product designed to detect and quantify the presence of cytochrome c, a protein involved in cellular respiration and apoptosis. This product provides a reliable and efficient method for researchers to investigate cellular processes and mechanisms related to cytochrome c.

Automatically generated - may contain errors

Lab products found in correlation

Anti cox 4, by Santa Cruz Biotechnology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Anti bax, by Merck Group (1 mentions) Bongkrekic acid, by Santa Cruz Biotechnology (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt, by Roche (1 mentions) Anti aif, by Cell Signaling Technology (1 mentions) α tubulin, by Novus Biologicals (1 mentions) Anti bax n 20, by Santa Cruz Biotechnology (1 mentions) Cyclosporine a, by Merck Group (1 mentions) Anti bcl 2, by Abcam (1 mentions) Anti β actin, by Abcam (1 mentions) Anti cleaved parp, by Merck Group (1 mentions) Anti survivin, by Novus Biologicals (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Protease and phosphatase inhibitor cocktail, by Roche (1 mentions) Nitrocellulose membrane, by Merck Group (1 mentions) Anti gapdh antibody, by Cell Signaling Technology (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions) Anti sirt1, by Merck Group (1 mentions)

2 protocols using anti cyto c

Apoptosis Induction and Mitochondrial Dysfunction

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The following reagents and antibodies were used: Bongkrekic acid (Santa Cruz Biotechnology, La Jolla, CA), JC-1 (eBioscience, San Diego, CA), Cyclosporine A, 2′,7′-dichlorofluorescein acetate (DCFH-DA), N-acetyl cysteine (Sigma, St.Louis, MO), Dioleylphosphatidylserine (Avanti Lipids, Alabaster, AL), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ((Roche Diagnostics, Indianapolis, IN), and disuccinyl suberate (Thermo Scientific Fischer, Rockford, IL). Anti-Bcl-2, anti-β-Actin (Abcam, Cambridge, MA), anti-Cyto c (eBioscience, San Diego, CA), anti-AIF, anti-caspase-3, anti-cleaved caspase-3 (Cell Signaling Technology, Boston, MA, anti-Survivin, Smac/Diablo, α-Tubulin (Novus biological, Littleton, CO), anti-COX-4, anti-Bax (N-20; Santa Cruz Biotechnology, La Jolla, CA), anti-Bax (polymer-recognizing A67 clone; Sigma, St.Louis, MO) and anti- cleaved PARP (Millipore, Bedford, MA).
Animal maintenance and experimental procedures were carried out in accordance with the US National Institute of health Guidelines for Use of Experimental Animals and approved by the Institutional Animal Care and Use Committee of the University of Cincinnati and Cincinnati Children's Hospital Medical Center.

Sulaiman M.K., Chu Z., Blanco V.M., Vallabhapurapu S.D., Franco R.S, & Qi X. (2015). SapC-DOPS nanovesicles induce Smac- and Bax-dependent apoptosis through mitochondrial activation in neuroblastomas. Molecular Cancer, 14, 78.

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Protein Expression Analysis Protocol

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The total protein extracts were prepared as described previously.29 (link) In brief, cells were lysed in cold radioimmunoprecipitation assay buffer with protease and phosphatase inhibitor cocktail (Roche). Equal amounts of protein samples were separated on SDS-PAGE, and then transferred onto nitrocellulose membranes (Millipore, Billerica, MA, USA). Then, membranes were probed with anti-PGC-1α (1:2,000; Santa Cruz Biotechnology, Dallas, TX, USA), anti-TFAM (1:16,000; Novus Bio, Littleton, CO, USA), anti-NRF1 (1:1,000; Santa Cruz Biotechnology), anti-SIRT1 (1:2,000; Sigma-Aldrich, St Louis, MO, USA), anti-cyto c (1:2,000; eBioscience, San Diego, CA, USA), anti-DIABLO (1:1,000; Cell Signaling Technologies, Danvers, MA, USA), and anti-GAPDH antibodies (1:1,000; Cell Signaling Technologies). The blots were then analyzed by chemiluminescence detection (ECL, Amersham, GE Healthcare Life Sciences, Chicago, IL, USA).

Bu X., Wu D., Lu X., Yang L., Xu X., Wang J, & Tang J. (2017). Role of SIRT1/PGC-1α in mitochondrial oxidative stress in autistic spectrum disorder. Neuropsychiatric Disease and Treatment, 13, 1633-1645.

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