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Allophycocyanin tcrβ

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Allophycocyanin-TCRβ is a fluorescent dye conjugate that is used in flow cytometry and other immunological applications to detect and analyze T cell receptor beta chain (TCRβ) expression on cell surfaces.

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Lab products found in correlation

Facsaria 3 sorp, by BD (2 mentions) Robosep, by STEMCELL (2 mentions)

2 protocols using allophycocyanin tcrβ

1

Isolation and Analysis of T Cell Subsets

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Single-cell lymphocyte solutions were prepared from organs as indicated and resuspended in staining buffer containing 5% FBS in HBBS. Pre-enrichment of splenic lymphocytes was performed using PE-labeled CD8+ T cell magnetic bead positive selection according to the manufacturer instructions (RoboSep, STEMCELL Technologies). Splenic and brain-resident lymphocytes were identified using mAbs against the following Ags, all from BioLegend unless otherwise noted: PE-CD8α (MAR1), FITC-CD4 (RM4–5), BV711-CD11b (M1/70), BV421-CD45.2 (1D4), allophycocyanin/Cy7-CD45.2 (1D4), and allophycocyanin-TCRβ, (H57–597; eBio science). Samples were sorted on an FACSAria III SORP (BD Biosciences) equipped with violet (403 nm, 100 mW), blue (488 nm, 100 mW), yellow-green (561 nm, 50 mW), and red (638 nm, 150 mW) lasers, then analyzed with FlowJo (Tree Star). Pre- and postsort purity of CD8+ T cells are shown in Fig. 1A.
Morawski P.A, & Bolland S. (2019). Strong Selection of a Few Dominant CD8 Clones in a TLR7-Dependent Autoimmune Mouse Model. ImmunoHorizons, 3(2), 61-70.
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2

Isolation and Analysis of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell lymphocyte solutions were prepared from organs as indicated and resuspended in staining buffer containing 5% FBS in HBBS. Pre-enrichment of splenic lymphocytes was performed using PE-labeled CD8+ T cell magnetic bead positive selection according to the manufacturer instructions (RoboSep, STEMCELL Technologies). Splenic and brain-resident lymphocytes were identified using mAbs against the following Ags, all from BioLegend unless otherwise noted: PE-CD8α (MAR1), FITC-CD4 (RM4–5), BV711-CD11b (M1/70), BV421-CD45.2 (1D4), allophycocyanin/Cy7-CD45.2 (1D4), and allophycocyanin-TCRβ, (H57–597; eBio science). Samples were sorted on an FACSAria III SORP (BD Biosciences) equipped with violet (403 nm, 100 mW), blue (488 nm, 100 mW), yellow-green (561 nm, 50 mW), and red (638 nm, 150 mW) lasers, then analyzed with FlowJo (Tree Star). Pre- and postsort purity of CD8+ T cells are shown in Fig. 1A.
Krebs C.J., Jarvis E.D., Chan J., Lydon J.P., Ogawa S, & Pfaff D.W. (2000). A membrane-associated progesterone-binding protein, 25-Dx, is regulated by progesterone in brain regions involved in female reproductive behaviors. Proceedings of the National Academy of Sciences of the United States of America, 97(23), 12816-12821.
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