To investigate the distribution of collagen fiber, Masson trichrome staining kit (D026; Nanjing Jiancheng Bioengineering Institute, Nanjing, China) was applied on lacrimal gland tissues. In brief, paraffin sections were dewaxed before staining. Tissues were rinsed with double distilled water for two minutes at 37°C, followed by nuclear staining, cytoplasm staining, color separation, and counterstaining according to the manufacturer's instructions. After douching with pure ethyl alcohol, sections were mounted with mounting medium (H-5000; Vector Laboratories, Burlingame, CA, USA) and observed under a light microscope.
Masson trichrome staining kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Masson Trichrome Staining Kit is a laboratory tool used for the histological staining of tissue samples. It is designed to differentiate between collagen, muscle, and other connective tissues within the sample. The kit includes the necessary staining reagents and protocols to perform this specialized staining technique.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using masson trichrome staining kit
1
Collagen Fiber Distribution in Lacrimal Gland
2
Masson Trichrome Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Masson Trichrome Staining Kit (Jiancheng Bioengineering Institute, Nanjing, China) was employed in this study. The sections were stained with Wiegert's iron hematoxylin, differentiated in an acid ethanol solution, and stained with Masson blue solution, followed by washing with DDW. Subsequently, the sections were stained with Lechon red magenta and washed with a phosphomolybdic acid solution before staining in a blue aniline solution. This was followed by gradient alcohol dehydration, xylene transparency, neutral gum sealing, and observation under a light microscope (Nikon).
3
Histological Analysis of Skin Transplantation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the day 14 post-transplantation, the SD rats were killed for histological analysis. Briefly, the ulcer wound bed together with surrounding tissue was excised and underwent following standard procedures including 4% paraformaldehyde fixation, gradual dehydration, and paraffin embedding. The embedded tissues were then sliced into 5-μm-thick sections in the direction of hair flow, which were further stained with Hematoxylin and Eosin Staining Kit (G1120, Solarbio, China) and Masson Trichrome Staining Kit (D026, Nanjing Jiancheng Bioengineering Institute, China). ImageJ software was used to measure the thickness of dermis, scar width, and the total number of hair follicles.
4
Histological Analysis of Wound Healing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the eighth day after surgery, the mice were sacrificed for histological analysis. Briefly, the wound bed together with surrounding tissue were excised and underwent following standard procedures including 4% paraformaldehyde fixation, gradual dehydration, and paraffin embedding. The embedded tissues were then sliced into 5-μm-thick sections in the direction of hair flow, which were further stained with a Hematoxylin and Eosin Staining Kit (G1120, Solarbio, China) to detect morphological changes and Masson Trichrome Staining Kit (D026, Nanjing Jiancheng Bioengineering Institute, China) to evaluate the synthesis of collagen. Image J software was used to measure the thickness of the dermis, the scar width, and the total number of hair follicles.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!