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4 protocols using gold chloride trihydrate

1

Synthesis of Multifunctional Nanoparticles

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Gold chloride trihydrate (HAuCl4·3H2O, 99.9%), sodium borohydride (NaBH4, 98%), trisodium citrate (99.5%), gadolinium chloride (GdCl3, 99%), hydrochloric acid (HCl, 36.0–38.0%), nitric acid (HNO3, 65.0–68.0%), silver nitrate (AgNO3, 99.8%), l-ascorbic acid (99.7%), sodium hydroxide (NaOH, 96.0%), cyclohexane (99.7%), tetraethyl orthosilicate and ethanol absolute (C2H6O, 99.7%) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Triethanolamine (TEA, AR), bromoacetic acid (AR), hexadecyltrimethylammonium bromide (CTAB, 99%) and cetyltrimethylammonium (CTAC, 97%) were bought from Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). 3-(Trimethoxysilylpropyl)diethylene triamine was purchased from Gelest (Morrisville, US). Fetal bovine serum (FBS, Gibco), phosphate buffered saline (PBS), trypsin–EDTA and Dulbecco's modified Eagle medium (DMEM) were obtained from Beyotime Biotechnology (Nantong, China).
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2

Synthesis of Gold Nanoparticles with PVP

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Gold chloride trihydrate (HAuCl4·3H2O; ≥99.9% trace metal basis; Sinopharm Chemical Reagent Co., Ltd, Shanghai, China), deionized water (18.2 MΩ cm), sodium hydroxide (NaOH; 98–99.5%; Aladdin, Shanghai, China), hydrogen peroxide (H2O2; ≈30% (v/v); Aladdin, Shanghai, China), polyvinyl pyrrolidone (PVP; MW 10 000, 40 000, 360 000; Sigma‐Aldrich, St. Louis, MO), hydrochloric acid (Sinopharm Chemical Reagent Co., Ltd, Shanghai, China), nitric acid (HNO3; AR; Sinopharm Chemical Reagent Co., Ltd, Shanghai, China), ethyl alcohol (C2H6O; AR; Sinopharm Chemical Reagent Co., Ltd, Shanghai, China), and near‐infrared‐dye 3,3′‐diethylthiatricarbocyanine iodide (DTTC; 99%; Sigma‐Aldrich, St. Louis, MO) were purchased.
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3

Synthesis of Nanoparticles using Detailed Reagents

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Trisodium citrate (≥99.9%), Sodium silicate solution (27%), (3-Aminopropyl)trimethoxysilane (APTMS, 97%), ascorbic acid (AA, >99.5%) and silver nitrate (AgNO3, >99.9%) were purchased from Sigma-Aldrich. Gold chloride trihydrate (HAuCl4⋅3H2O, >99.9%), hydrochloric acid (HCl, >98.0%), Rhodamine 6G (R6G, >99.0%) were purchased from sinopharm chemical reagent Co. Ltd. Ultrapure water from Milli-Q (Millipore, America, resistivity >18.4 MΩ⋅cm) source was used throughout the experiments. All materials were used without further purification.
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4

Synthesis of Gold Nanoparticles

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Gold chloride trihydrate (HAuCl4 · 3H2O, >99.9%), sodium borohydride (NaBH4, >96%), cetyltrimethylammonium bromide (CTAB, >99.0%), polyvinyl-pyrrolidone (PVP, >99.0%), and ascorbic acid (AA, >99.5%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and used without further purification. Ultrapure water (resistivity >18.0 MΩ cm) was used throughout the experiments.
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