To diminish the influence of cytokines present in FCS, both confluent pRMG and MIO-M1 cells were rinsed two times with prewarmed serum-free medium, followed by starvation for 1 h at 37°C and 5% CO2 with serum-deprived medium. Afterwards, cells were treated over night with IFNy, IL-4, IL-6, IL-10, TGFβ1, TGFβ2, TGFβ3, TNFα or VEGF165, respectively, in a randomized plate design at a concentration of 5 ng/ml in 2 ml medium without FCS. Untreated cells cultured in serum-free medium served as a control. For this study, cells were treated with each cytokine separately, but not with multiple cytokines in combination.
Vascular endothelial growth factor165
Vascular Endothelial Growth Factor165 (VEGF) is a recombinant protein that stimulates the growth and migration of endothelial cells. It plays a crucial role in angiogenesis, the process of new blood vessel formation.
Lab products found in correlation
2 protocols using vascular endothelial growth factor165
Cytokine Stimulation of Retinal Cells
To diminish the influence of cytokines present in FCS, both confluent pRMG and MIO-M1 cells were rinsed two times with prewarmed serum-free medium, followed by starvation for 1 h at 37°C and 5% CO2 with serum-deprived medium. Afterwards, cells were treated over night with IFNy, IL-4, IL-6, IL-10, TGFβ1, TGFβ2, TGFβ3, TNFα or VEGF165, respectively, in a randomized plate design at a concentration of 5 ng/ml in 2 ml medium without FCS. Untreated cells cultured in serum-free medium served as a control. For this study, cells were treated with each cytokine separately, but not with multiple cytokines in combination.
Immunofluorescence Analysis of Angiogenic Factors
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