Agilent msd 6130

For the ADME property determination, an Agilent 1260 Infinity HPLC-DAD interfaced with an Agilent MSD 6130 (Agilent Technologies, Palo Alto, CA, USA) was used. Chromatographic conditions were optimized using a Phenomenex Kinetex C18-100 Å column (150 × 4.6 mm) with 5 µm particle size and gradient elution with a binary solution (eluent A: H₂O; eluent B: ACN; both eluents were acidified with formic acid 0.1% v/v) at room temperature. The LC analyses started with 5% of B (from t = 0 to t = 1 min), then B was increased to 95% (from t = 1 to t = 10 min) and then kept at 95% (from t = 10 to t = 19 min) and finally returned to initial conditions in 1.0 min. The flow rate was 0.6 mL/min and the injection volume was 10 µL.

UV/LC–MS LC analyses
for ADME studies were performed by UV/LC–MS with an Agilent
1260 Infinity HPLC-DAD system interfaced with an Agilent MSD 6130
(Agilent Technologies, Palo Alto, CA) system. Chromatographic separation
was obtained using a Phenomenex Kinetex C18-100 Å column (150
× 4.6 mm) with 5 μm particle size and gradient elution
with a binary solution (eluent A: H₂O, eluent B: ACN, both
eluents were acidified with formic acid 0.1% v/v) at room temperature.
The analysis started with 5% of B (from t = 0 to t = 1 min), then B was increased to 95% (from t = 1 to t = 10 min), then kept at 95% (from t = 10 to t = 19 min), and finally return
to 5% of eluent A in 1.0 min. The flow rate was 0.6 mL/min, and injection
volumes were 10 μL.