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Percp cy5 5 thy1

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PerCP/Cy5.5-Thy1.1 is a fluorescent label used in flow cytometry. It is a tandem dye consisting of the peridinin-chlorophyll protein (PerCP) and the cyanine dye Cy5.5, which is conjugated to the Thy1.1 antibody. The PerCP/Cy5.5 label emits light in the far-red region of the visible spectrum and is commonly used for multicolor flow cytometry applications.

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Apc tcr β, by BD (2 mentions) Cd11c pe cy7, by BD (2 mentions) Cd25 apc, by BD (2 mentions) Foxp3 pe, by BD (2 mentions) Fitc sirpα, by BD (2 mentions) Brilliant violet 605 cd11c, by BD (2 mentions) Pacific blue ia ie, by BD (2 mentions) Pacific blue foxp3, by BD (2 mentions) Cd8α fitc, by BD (2 mentions) Alexa700 cd8α, by BD (2 mentions) Brilliant violet 605 cd4, by BD (2 mentions) Brilliant violet 605 cd25, by BD (2 mentions) Cd24 pe cy7, by BD (2 mentions) Biotin cd8β, by BD (2 mentions) Biotin cd25, by BD (2 mentions) B220 biotin, by BD (2 mentions) Propidium iodide solution, by BD (1 mentions) Cd86 apc, by BD (1 mentions) Cd86 pe cy7, by BD (1 mentions) Alexa fluor 647 conjugated ova, by Thermo Fisher Scientific (1 mentions)

2 protocols using percp cy5 5 thy1

1

Multiparameter Immune Cell Analysis

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The following antibodies were purchased from BioLegend, eBioscience, or BD Biosciences, and used for flow cytometry or enrichment of specific cell types: FITC-Sirpα, PE/Cy7-Sirpα PerCP/Cy5.5-B220, PE/Cy7-CD11c, Brilliant Violet 605-CD11c, Pacific Blue-IA/IE, FITC-YAe, PerCP/Cy5.5-CD45.1, APC-CD45.2, PE/Cy7-CD86, APC-CD86, PE-PD-L1, APC-CD200, PE/Cy7-CD40, PE-Foxp3, Pacific Blue-Foxp3, PerCP/Cy5.5-Thy1.1, APC-TCRβ, FITC-CD8α, Alexa700-CD8α, Alexa700-CD4, Brilliant Violet 605-CD4, Brilliant Violet 605-CD25, APC-CD25, PE/Cy7-CD3, PE/Cy7-CD24, Propidium iodide solution, Fixable Viability Dye eFluor780, Biotin-CD8β, Biotin-CD4, Biotin-CD25, and Biotin-B220. AlexaFluor647-conjugated OVA and crimson bead (0.02 μm) were purchased from ThermoFisher Scientific. Streptavidin-RapidSpheres isolation kit and CD11c-MicroBeads were purchased from STEMCELL technologies and Miltenyi Biotec, respectively.
Oh J., Wu N., Barczak A.J., Barbeau R., Erle D.J, & Shin J.S. (2018). CD40 Mediates Maturation of Thymic Dendritic Cells Driven by Self-reactive CD4+ Thymocytes and Supports Development of Natural Regulatory T Cells. Journal of immunology (Baltimore, Md. : 1950), 200(4), 1399-1412.
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2

Immunophenotyping and Protein Analysis

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The following antibodies were purchased from BioLegend, eBioscience, or BD Biosciences and used for flow cytometry or enrichment: FITC-Sirpα, PE/Cy7-Sirpα, PerCP/Cy5.5-B220, APC-EpCAM, PE-CD11c, PE/Cy7-CD11c, Brilliant Violet 605-CD11c, Pacific Blue-IA/IE, FITC-CD8α, Alexa700-CD8α, PE-Vα2, APC-Vα2, APC-Vβ5, PE-Vβ6, PE-Foxp3, Pacific Blue-Foxp3, PerCP/Cy5.5-Thy1.1, APC-TCRβ, PE-CD69, PE/Cy7-CD69, Brilliant Violet 605-CD4, APC-CD25, Brilliant Violet 605-CD25, Brilliant Violet 785-CD25, FITC-CD45RB, APC-ICAM1, PR-CD9, PE-CD81, APC-CD48, PE/Cy7-CD24, APC-CD24, PE-CD71, PE/Cy7-CD45, APC-streptavidin, Biotin-Thy1.2, Biotin-CD8β, Biotin-CD25, and Biotin-B220. Alexa647-CTxB subunit was purchased from Molecular Probes. For Western blotting, the following clones were used: MHCII β chain (Bett; homemade), CD48 (OX-78; Serotec), CD24 (M1/69; BioLegend), actin (A2066; Sigma), CD9 (LMC8; eBioscience), and CD81 (Eat2; BioLegend), and HRP-conjugated secondary antibodies (anti-rat, anti-rabbit, and anti-hamster) were purchased from Jackson ImmunoResearch or BIOSOURCE. Myriocin, MβCD, cholesterol, sphingomyelin, and 4-hydroxy-tamoxifen were purchased from Sigma.
Oh J., Perry J.S., Pua H., Irgens-Möller N., Ishido S., Hsieh C.S, & Shin J.S. (2018). MARCH1 protects the lipid raft and tetraspanin web from MHCII proteotoxicity in dendritic cells. The Journal of Cell Biology, 217(4), 1395-1410.
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