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Rpm1 1640 medium

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RPMI 1640 medium is a cell culture medium used to support the growth and maintenance of a wide variety of cell lines. It provides the necessary nutrients and components to support cell viability and proliferation. The medium is commonly used in various applications, including biomedical research, cell and tissue culture experiments, and cell-based assays.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Hyclone antibiotic antimycotic solution, by GE Healthcare (2 mentions) Glutamax 1 and sodium pyruvate, by GE Healthcare (2 mentions) Hydrocortisone hc, by Merck Group (2 mentions) Dmem medium, by GE Healthcare (2 mentions) Dmem f12 medium, by Biosera (2 mentions) Egf epithelial growth factor, by Merck Group (2 mentions) Insulin, by Merck Group (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mcf 10a, by Biosera (1 mentions) Mcf 7, by GE Healthcare (1 mentions) Mda mb 231, by Biosera (1 mentions)

4 protocols using rpm1 1640 medium

1

Cytotoxic Effects of Thyme Essential Oil

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The human cancer cell line MCF-7 (human breast adenocarcinoma; ER+, PR+, HER2-) and MDA-MB-231 (human breast adenocarcinoma; ER-, PR-, HER2-) were cultured in Dulbecco’s modified Eagle’s medium with Glutamax-I and Sodium pyruvate (GE Healthcare, Piscataway, NJ, USA) and RPM1 1640 medium (Biosera, Kansas City, MO, USA), respectively. The growth medium was supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific and 1X HyClone™ Antibiotic/Antimycotic Solution (GE Healthcare), and cells were cultivated in an atmosphere containing 5% CO2 in humidified air at 37 °C. Cell viability, estimated by trypan blue exclusion, was greater than 95% before each experiment.
MCF-7 (3 × 105) and MDA-MB-231 (1 × 105) cells were seeded in Petri dishes and cultivated for 24 h in a complete medium with 10% FCS. Cells were treated with essential oil of T. vulgaris (EOT, Calendula, Nová Ľubovňa, Slovak Republic) for 24, 48, and 72 h prior to analysis. EOT was prepared by a steam distillation procedure. Its composition was analyzed by GC-MS, as is described later.
Kubatka P., Uramova S., Kello M., Kajo K., Samec M., Jasek K., Vybohova D., Liskova A., Mojzis J., Adamkov M., Zubor P., Smejkal K., Svajdlenka E., Solar P., Samuel S.M., Zulli A., Kassayova M., Lasabova Z., Kwon T.K., Pec M., Danko J, & Büsselberg D. (2019). Anticancer Activities of Thymus vulgaris L. in Experimental Breast Carcinoma In Vivo and In Vitro. International Journal of Molecular Sciences, 20(7), 1749.
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2

Sumac Extract's Effects on Breast Cancer Cells

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In the in vitro experiments, human breast cancer cell lines MCF-7 (ER+, PR+, HER2−), MDA-MB-231 (ER− PR−, HER2−) and non-cancer MCF-10A (human mammary gland epithelial cells) were used. Breast cancer cells were cultured in DMEM medium (GE Healthcare, Piscataway, NJ, USA) or RPM1 1640 medium (Biosera, Kansas City, MO, USA) and non-cancer cells were cultured in DMEM F12 medium (Biosera, Kansas City, MO, USA) + supplemented with insulin, EGF- epithelial growth factor, HC-hydrocortisone (all Sigma, Steinheim, Germany). The growth medium was supplemented with 10% FBS (Gibco), antibiotic/antimycotic solution (1× HyClone™; GE Healthcare, Chicago, IL, USA) and cells were cultivated in an atmosphere containing 5% CO2 in humidified air at 37 °C. Before each experiments, the cell viability was estimated by trypan blue exclusion (≥95%).
For the flow cytometry experiments, the MCF-7 (3 × 105) and MDA-MB-231 (1 × 105) cells were seeded in Petri dishes and cultivated for 24 h in a complete cultivation medium. The sumac extract (SONNENTOR Kräuterhandels GMBH, Sprögnitz, Austria) was added to every experimental group for 24, 48, and 72 h prior to analysis.
Kubatka P., Kello M., Kajo K., Samec M., Liskova A., Jasek K., Koklesova L., Kuruc T., Adamkov M., Smejkal K., Svajdlenka E., Solar P., Pec M., Büsselberg D., Sadlonova V, & Mojzis J. (2020). Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma. International Journal of Molecular Sciences, 22(1), 183.
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3

Cytotoxicity of Essential Oil Components

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The human cancer cell line MCF-7 (human breast adenocarcinoma, ER+, PR+, HER2-) and MDA-MB-231 (human breast adenocarcinoma; ER-, PR-, HER2-) were cultured in Dulbecco’s modified Eagle’s medium with Glutamax-I and Sodium pyruvate (GE Healthcare, Piscataway, NJ, USA) or RPM1 1640 medium (Biosera, Kansas City, MO, USA), respectively. The growth medium was supplemented with a 10% fetal bovine serum (Gibco), 1X HyClone™ Antibiotic/Antimycotic Solution (GE Healthcare) and cells were cultivated in an atmosphere containing 5% CO2 in humidified air at 37 °C. Cell viability, estimated by trypan blue exclusion, was greater than 95% before each experiment.
MCF-7 (3 × 105) and MDA-MB-231 (1 × 105) cells were seeded in Petri dishes and cultivated for 24 h in a complete medium with 10% FCS. Cells were treated with EOC (Calendula, Nová Ľubovňa, Slovak Republic) for 24, 48, and 72 h prior to analysis.
Kubatka P., Kello M., Kajo K., Samec M., Jasek K., Vybohova D., Uramova S., Liskova A., Sadlonova V., Koklesova L., Murin R., Adamkov M., Smejkal K., Svajdlenka E., Solar P., Samuel S.M., Kassayova M., Kwon T.K., Zubor P., Pec M., Danko J., Büsselberg D, & Mojzis J. (2020). Chemopreventive and Therapeutic Efficacy of Cinnamomum zeylanicum L. Bark in Experimental Breast Carcinoma: Mechanistic In Vivo and In Vitro Analyses. Molecules, 25(6), 1399.
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4

Breast Cancer Cell Line Cultivation and Treatment

Cited 3 times
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BC cell lines MCF-7 (ER+, PR+, HER2−) and MDA-MB-231 (ER−, PR−, HER2−) (both lines: American Type Culture Collection, ATCC; Manassas, VA, USA) were used in all in vitro experiments. In addition, a non-cancerous MCF-10A (human mammary gland epithelial cells) were used as a normal breast epithelial model. MCF-7 cells were maintained in DMEM medium (GE Healthcare, Piscataway, NJ, USA), MDA-MB-231 in RPM1 1640 medium (Biosera, Kansas City, MO, USA), and MCF-10A in DMEM F12 medium (Biosera, Kansas City, MO, USA) + suppl. insulin, EGF- epithelial growth factor and HC-hydrocortisone (all Sigma, Steinheim, Germany). The 10% FBS (Fetal bovine serum, Gibco) and antibiotic/antimycotic solution (1× HyClone™; GE Healthcare, Chicago, IL, USA) were used to supplement culture media. Basic cultivation conditions were a 5% CO2-containing atmosphere, humidified air, and 37°C temperature. A trypan blue exclusion test was used to estimate the viability of used cells (≥95%). For the flow cytometry experiments, the MCF-7 (3 × 105) and MDA-MB-231 (1 × 105) cells were seeded in Petri dishes. After 24 h seeding and initial colony growth in a complete cultivation medium, the cells were treated with the SPGE (Calendula, Nová Ľubovňa, Slovakia) for 24, 48, and 72 h before analysis (Kubatka et al., 2019 (link); Kubatka et al., 2020a (link); Kubatka et al., 2020b (link)).
Kubatka P., Mazurakova A., Koklesova L., Kuruc T., Samec M., Kajo K., Kotorova K., Adamkov M., Smejkal K., Svajdlenka E., Dvorska D., Brany D., Baranovicova E., Sadlonova V., Mojzis J, & Kello M. (2024). Salvia officinalis L. exerts oncostatic effects in rodent and in vitro models of breast carcinoma. Frontiers in Pharmacology, 15, 1216199.
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