Hsd:Athymic Nude-Foxn1nu female mice (6 weeks of age) for xenograft experiments were purchased from Envigo. Mice were housed in a controlled environment (12-h light/12-h dark cycle, light intensity ~350 lux) with ad libitum access to water and standard food (13.5% kcal from fat, LabDiet 5L0D). All animal experiments were performed in compliance with the NIH Guide for the Care and Use of Laboratory Animals and Animal Welfare Act. All animal studies were approved and performed in accordance with the Vanderbilt Institutional Animal Care and Use Committee.
Hsd athymic nude foxn1nu female mice
Manufactured by Inotiv
Sourced in United States
The Hsd:Athymic Nude-Foxn1nu female mice are a genetically modified mouse model with a congenital absence of thymus, resulting in a lack of mature T cells. This model is commonly used in research applications requiring immunocompromised animals.
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3 protocols using hsd athymic nude foxn1nu female mice
1
Xenograft Experiments with Athymic Nude Mice
2
Nude Mice Xenograft Tumor Model
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All animal experiments were conducted under a UT-Southwestern IACUC approved protocol. Hsd:Athymic Nude-Foxn1nu female mice were purchased from Envigo. At six to eight-weeks of age (23–25 grams) mice were randomly assigned to the indicated groups. Sample size was determined by the formula: n = 1+2C(s/d)2, in which C = 10.51, based on a power of 90% and a confidence interval of 95%, S = standard deviation of tumor volume (20%), D = tumor burden difference to be considered significant (50%). Therefore, five mice per condition were tested. 2 × 106 cells in 100 µl PBS were subcutaneously injected in the flank. Once tumors were visible, tumor volume was measured by caliper in two dimensions, and volumes were estimated using the equation V = length x width2/2. Caliper measurements were made twice a week.
3
Xenograft Tumor Induction in Athymic Mice
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Six-week-old Hsd:Athymic Nude-Foxn1nu female mice (Envigo, Indianapolis, IN, USA) were purchased and acclimatized for 1 week. Bilateral flank tumors were generated by injecting 2–5 × 106 HCT116 or HT29 cells in a total of 100 μL PBS containing 50% matrigel for each tumor. When average tumor size approached 150 mm3, mice were divided into experimental groups.
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