Emem medium

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

EMEM medium is a culture medium used for the growth and maintenance of various cell lines in vitro. It provides the necessary nutrients and components to support cell growth and proliferation. The medium is formulated to maintain the optimal pH and osmotic environment for cells.

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Lab products found in correlation

28 protocols using emem medium

Antimicrobial Efficacy of Zinc Nitrate

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Zinc nitrate hexahydrate (ZnNO₃·6H₂O) with a 98% analytical grade was purchased from LOBACHEMIE PVT.LTD (Mumbai, India). A marketed hand sanitizer product (Sterillium^®) manufactured by the HARTMANN GROUP’s manufacturing company (Germany) was purchased from a local store. All other reagents were supplied by Sigma-Aldrich (Germany). All chemicals and reagents were of analytical grade. Double-distilled water was obtained from the New Water Purification System (New Human Power I, VER. 2.0, Seoul, Korea)
Microbiological media and all chemical reagents were purchased from Oxoid, UK. Four hospital-acquired human pathogenic bacteria, namely, Acinetobacter baumannii (A. baumannii), Klebsiella pneumoniae (K. pneumoniae), Methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus (S. aureus), were collected from a clinical setting in Cairo, Egypt. They were identified as multidrug-resistant bacteria.
The adult human dermal fibroblast (HDFa) cell line (PCS-201-012, HDFa) was purchased from the American Type Culture Collection (ATCC). The EMEM medium, fetal bovine serum (FBS), non-essential amino acids (NEAAs), penicillin, and streptomycin were supplied by Thermo Fisher (Hessen, Germany). The MTT reagent was supplied by Thermo Fisher, Germany. All other materials were purchased from Sigma-Aldrich and used as supplied.

Ismail A., Raya N.R., Orabi A., Ali A.M, & Abo-zeid Y. (2022). Investigating the Antibacterial Activity and Safety of Zinc Oxide Nanoparticles versus a Commercial Alcohol-Based Hand-Sanitizer: Can Zinc Oxide Nanoparticles Be Useful for Hand Sanitation?. Antibiotics, 11(11), 1606.

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Antibacterial and Cytotoxicity Evaluation

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Microbiological media (tryptone soy broth; TSB, and tryptone soy agar; TSA) were purchased from Hi-Media, India. Peptone and sodium chloride were purchased from Oxoid, UK. Hydrochloric acid, ethyl acetate, and sulfuric acid were purchased from Honeywell™, Charlotte, NC, USA. l-rhamnose was purchased from Sigma-Aldrich, Darmstadt, Germany. Orcinol was obtained from SDFCL, Chennai, India. Carbopol gel, phosphate-buffered saline (PBS) tablets, and absolute ethyl alcohol were purchased from Merck, Darmstadt, Germany. All chemicals and reagents were of analytical grade.
Two hospital-acquired human pathogenic bacteria—Acinetobacter baumannii and Staphylococcus aureus—were collected from a clinical setting in Cairo, Egypt. They were identified as multidrug-resistant bacteria.
The adult human dermal fibroblast (HDFa) cell line (PCS-201-012, HDFa) was purchased from the American Type Culture Collection (ATCC). EMEM medium, fetal bovine serum (FBS), non-essential amino acids (NEAAs), penicillin, and streptomycin were supplied by Thermo Fisher (Hessen, Germany). MTT reagent was supplied by Thermo Fisher, Germany. All other materials were purchased from Sigma-Aldrich and used as supplied.

Abo-zeid Y., Bakkar M.R., Elkhouly G.E., Raya N.R, & Zaafar D. (2022). Rhamnolipid Nano-Micelles versus Alcohol-Based Hand Sanitizer: A Comparative Study for Antibacterial Activity against Hospital-Acquired Infections and Toxicity Concerns. Antibiotics, 11(5), 605.

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Investigating MUC1-C Inhibition in BRAF(V600E) Cells

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RKO BRAF(V600E) cells (ATCC, Manassas, VA, USA) were cultured in EMEM medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; GEMINI Bio-Products, West Sacramento, CA, USA). LS411N BRAF(V600E) cells (ATCC) were cultured in RPMI1640 medium supplemented with 10% FBS. Cells were treated with the MUC1-C inhibitor GO-203 (13 (link)–15 (link)). Authentication of the cells was performed by short tandem repeat (STR) analysis. Cells were monitored for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, MA, USA). Cells were maintained for 3 months when performing experiments.

Morimoto Y., Yamashita N., Hirose H., Fushimi A., Haratake N., Daimon T., Bhattacharya A., Ahmad R., Suzuki Y., Takahashi H, & Kufe D.W. (2023). MUC1-C IS NECESSARY FOR SHP2 ACTIVATION AND BRAF INHIBITOR RESISTANCE IN BRAF(V600E) MUTANT COLORECTAL CANCER. Cancer letters, 559, 216116.

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Breast Cancer Cell Line Culture Protocols

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SUM149 and SUM159-Luc cells were cultured in Ham’s F-12 (Invitrogen) supplemented with 5% FBS (ThermoFisher Scientific, Pittsburgh, PA), 5 μg/mL insulin, and 1 μg/mL hydrocortisone (both from Sigma, St. Louis, MO), 10 μg/ml gentimycin, and 1% antibiotic-antimycotic (both from Invitrogen). MCF-7 was maintained in EMEM medium (ThermoFisher Scientific) supplemented with 5% FBS, 1% antibiotic-antimycotic (both from ThermoFisher Scientific), and 5 μg/ml insulin (Sigma, St Louis, MO). HCC1806, HCC1937 and T47D were maintained in RPMI1640 medium (ThermoFisher Scientific) supplemented with 10% FBS and 1% antibiotic-antimycotic. All cell lines were developed from female breast cancer patients and are cultured in an incubator at 37°C with 5% CO_2.

Luo M., Shang L., Brooks M.D., Jiagge E., Zhu Y., Buschhaus J.M., Conley S., Fath M.A., Davis A., Gheordunescu E., Wang Y., Harouaka R., Lozier A., Triner D., McDermott S., Merajver S.D., Luker G.D., Spitz D.R, & Wicha M.S. (2018). Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling. Cell metabolism, 28(1), 69-86.e6.

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Culturing PSMA-Positive Prostate Cancer Cells

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Human PSMA-positive (PSMA+) prostate cancer cell lines LNCaP, C4-2, PC-3-PSMA+, and PSMA-negative (PSMA-) prostate cancer cell line DU-145, PC-3 cells were maintained in F-12K, DMEM, RPMI-1640, or EMEM medium (Gibco Life Technologies, Paisley, Scotland, UK) supplemented with 1% penicillin-streptomycin (Invitrogen Life Technologies, USA) and10% fetal bovine serum (Gibco Life Technologies, USA). The cells were cultured at 37°C with 5% CO₂ in a humidified incubator.

Shi S.J., Wang L.J., Han D.H., Wu J.H., Jiao D., Zhang K.L., Chen J.W., Li Y., Yang F., Zhang J.L., Zheng G.X., Yang A.G., Zhao A.Z., Qin W.J, & Wen W.H. (2019). Therapeutic effects of human monoclonal PSMA antibody-mediated TRIM24 siRNA delivery in PSMA-positive castration-resistant prostate cancer. Theranostics, 9(5), 1247-1263.

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Acer mono Extract Cytotoxicity Evaluation

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An extract of the medicinal plant Acer mono was purchased from Plant Extract Bank, Daejeon, Korea. The extract was dissolved in methanol and stored at 4℃. EMEM medium, trypsin-EDTA, and fetal bovine serum (FBS) were obtained from Gibco BRL (Life Technologies, USA). HT1080 fibrosarcoma cells were obtained from the American Type Culture Collection (USA). Antibodies were purchased from R&D (USA), and matrigel was purchased from BD Biosciences (USA).

Kim J.H., Hwang G.H., Kim H.J., Jeon S, & Shin B.A. (2021). Acer mono Extract Inhibits Invasive Activities and G1/S Transition of HT1080 Fibrosarcoma Cells. Chonnam Medical Journal, 57(3), 185-190.

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Culturing KRAS Lung Cancer Cell Lines

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KRAS wildtype lung cancer cell lines (NCIH3122, NCIH2023, HCC827, NCIH1755, NCIH1299, NCIH1781 and NCIH1563) and KRAS wildtype lung cancer cell lines (CORL23, LU65, NCIH460, NCIH1734, NCIH358, SW900, A549 and LU99A) were cultured in RPMI medium (Gibco Co., Gaithersburg, MD, USA) containing penicillin/streptomycin and 15% heat-inactivated FBS (Gibco Co., Gaithersburg, MD, USA), as recommended by ATCC, at 37°C, 5% CO2, and 95% humidity. Normal lung fibroblast cells (HEL-299 and MRC-5) were cultured in EMEM medium (Gibco, Gaithersburg, MD, USA) containing penicillin/streptomycin and 10% FBS.

Hu J., Zhang Z., Zhao L., Li L., Zuo W, & Han L. (2019). High expression of RAD51 promotes DNA damage repair and survival in KRAS-mutant lung cancer cells. BMB Reports, 52(2), 151-156.

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Anticancer Peptide Evaluation Using LPPC

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Lipo-PEI-PEG-complex (LPPC) was provided by the lab of Dr. Kuang-Wen Liao, National Yang Ming Chiao Tung University, Hsinchu, Taiwan. Anticancer peptides (ACP) were synthesized and purchased from AngeneBiotech (Taipei, Taiwan). Phenol, chloroform, RIPA lysis buffer and protease inhibitor were purchased from Bio Basic Inc. (Toronto, Canada). MTT, propidium iodide (PI), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich Chemicals Co. (St. Louis, MO, USA). Pierce™ ECL Western Blotting Substrate was purchased from Thermo (Thermo Fisher Scientific Inc., Waltham, MA, USA). Myco5A medium, M199 medium, EMEM medium, DMEM medium, RPMI-1640 medium, fetal bovine serum (FBS), PBS buffer solution, sodium pyruvate (100 mM), and 0.05% trypsin-EDTA were purchased from Gibco (Grand Island, NY, USA).

Chen C.H., Weng T.H., Huang K.Y., Kao H.J., Liao K.W, & Weng S.L. (2022). Anticancer peptide Q7 suppresses the growth and migration of human endometrial cancer by inhibiting DHCR24 expression and modulating the AKT-mediated pathway. International Journal of Medical Sciences, 19(14), 2008-2021.

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Lentiviral Manipulation of ZNF32 in Neuroblastoma

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Human embryonic kidney cells (HEK293) and human neuroblastoma BE(2)-C (from American Type Culture Collection) and BE(2)-C stably cell lines were each maintained in DMEM and EMEM medium (Gibco) containing 10% fetal bovine serum (Gibco), 100 U/mL penicillin, 100 mg/mL streptomycin and 3.7 g/L NaHCO₃. Cells were maintained at 37°C in a humidified 95% air and 5% CO₂ atmosphere.
The ZNF32 lentiviral expression vector was constructed by inserting ZNF32 cDNA into the LV6 lentiviral shuttle vector, yielding LV6-ZNF32. ShRNA targeting ZNF32 was cloned to the lentiviral vector - LV2pGLVU6/GFP + Puro, yielding lv-ZNF32. Recombinant lentiviral plasmids were transfected into BE(2)-C cells. Stable BE(2)-C cell lines were selected with puromycin. The packing and purification of recombinant lentiviral vectors was performed by Shanghai GenePharma Company. The shRNA sequences were as follows: shRNA-ZNF32, 5′-GAATGTAGCGTTCTTCAATGT-32; shRNA-NC, 5′-TTCTCCGAACGTGTCAGGT-32. The pSG5 vector (pSG5-Vec) was stored in our lab, and the pSG5-ZNF32 over-expression plasmid (pSG5-ZNF32) was constructed by inserting expanded ZNF32 cDNA fragments into pSG5-Vec.

Wei Y., Li K., Yao S., Gao J., Li J., Shang Y., Zhang J., Zhang L., Li Y., Mo X., Meng W., Xiang R., Hu J., Lin P, & Wei Y. (2016). Loss of ZNF32 augments the regeneration of nervous lateral line system through negative regulation of SOX2 transcription. Oncotarget, 7(43), 70420-70436.

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Cell Characterization and Culture Conditions

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Human trophoblast cells (JEG3 and HTR-8) and human embryonic kidney 293 T (HEK-293 T) were selected for this study. Both JEG3 and HTR-8 showed epithelial-like phenotypes under a light microscope (Supplementary file 1). JEG3 cell line was obtained from Huatuo Biotechnology Co., Ltd. (Shenzhen, China) while HTR-8 and HEK-293 T cells were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). JEG3 cells were incubated in EMEM Medium containing 10% FBS (Gibco). HTR-8 cells were maintained in RPMI-1640 Medium containing 5% FBS while HEK-293 T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% FBS. All mediums were kept under the condition of 5% CO₂ at 37 °C. Cell cultures were regularly checked by polymerase chain reaction (PCR) for mycoplasma contamination, as previously described [19 (link)]. Additionally, the authentication of JEG3 and HTR-8 cells, as well as cross-contamination, was checked by STR profiling, and the corresponding STR reports were provided in Supplementary files 2 and 3.

Huo W., Wang Y., Chen T., Cao T., Zhang Y., Shi Z, & Hou S. (2022). Triclosan activates c-Jun/miR-218-1-3p/SLC35C1 signaling to regulate cell viability, migration, invasion and inflammatory response of trophoblast cells in vitro. BMC Pregnancy and Childbirth, 22, 470.

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