Stempro hesc human embryonic stem cell culture medium

The institutional ethics committees at the Osaka Medical Center for Cancer and Cardiovascular Diseases approved the present study. Human bladder tumor specimens were collected from patients treated at the Department of Urology, Osaka Medical Center for Cancer and Cardiovascular Diseases. CTOS were prepared and cultured according to the previously described protocol.22 (link),24 (link) Briefly, surgical samples or xenograft tumors from NOD/SCID mice were partially digested with Liberase DH (Roche, Mannheim, Germany) and filtered through cell strainers. Fragments were collected on 100 or 40-μm cell strainers (BD Falcon, Franklin Lakes, NJ, USA). CTOS were cultured in StemPro hESC human embryonic stem cell culture medium (Invitrogen, Carlsbad, CA, USA). The growth rate was calculated by dividing the area of CTOS at day 4 by that at day 1.

Urine samples were collected from patients diagnosed with urothelial cancer by cytology. Voided urine or urine from catheters was collected and centrifuged, and sediments were recovered and cultured in StemPro hESC human embryonic stem cell culture medium (Invitrogen). Subsequently, cell clusters in the culture medium were picked up using a pipette and further cultured in Matrigel growth factor reduced (BD Biosciences, Bedford, MA) or type I collagen–coated dishes for growth or adhesion assays, respectively.