Seven-week-old male Mongolian gerbils (Meriones unguiculatus) were purchased from DaehanBio (Eumsung, Korea) and acclimated in the animal facility for one week. They were raised in an individual cage at 23 °C, 60% humidity, and a 12 h light/dark cycle. They freely consumed food and water. The study protocols conformed to the National Institute of Health Guidelines and were approved by Hoseo University Animal Care and Use Committee (HSIAUC-18-065).
A semi-purified diet formulation for laboratory rodents as formulated by the American Institute of Nutrition in 1993 (AIN-93) was used to make an experimental diet [18 (link)]. The fat contents in AIN-93 were modified to 43 energy percent, and corn silk water extract (0.05% and 0.2%) was added to the 43 energy percent (En%) fat diet. For the I/R-aspirin, 0.02% aspirin (Bayer, Leverkusen, Germany) was added to the 43 En% fat diet as the positive control (I/R-aspirin). The corn silk water extracts and aspirin were substituted for the cellulose contents. According to the analysis results, each diet was tailored to contain identical total amounts of carbohydrates, protein, and lipids by changing the casein, soybean oil, and cellulose contents. All diets consisted of 43 En% carbohydrates, 17 En% protein, and 40 En% fats. Cellulose (3.4%), mineral (3.5%), and vitamin (1.0%) mixtures were added to all diets.
A semi-purified diet formulation for laboratory rodents as formulated by the American Institute of Nutrition in 1993 (AIN-93) was used to make an experimental diet [18 (link)]. The fat contents in AIN-93 were modified to 43 energy percent, and corn silk water extract (0.05% and 0.2%) was added to the 43 energy percent (En%) fat diet. For the I/R-aspirin, 0.02% aspirin (Bayer, Leverkusen, Germany) was added to the 43 En% fat diet as the positive control (I/R-aspirin). The corn silk water extracts and aspirin were substituted for the cellulose contents. According to the analysis results, each diet was tailored to contain identical total amounts of carbohydrates, protein, and lipids by changing the casein, soybean oil, and cellulose contents. All diets consisted of 43 En% carbohydrates, 17 En% protein, and 40 En% fats. Cellulose (3.4%), mineral (3.5%), and vitamin (1.0%) mixtures were added to all diets.