Eol 1

Manufactured by Leibniz Institute DSMZ

Sourced in Germany

The EOL-1 is a lab equipment product offered by the Leibniz Institute DSMZ. It is a device used for the counting and analysis of microorganisms. The core function of the EOL-1 is to facilitate the enumeration and characterization of microbial cells.

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Lab products found in correlation

12 protocols using eol 1

Cell Line Procurement and Culture

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HL-60 cells were obtained from NCI 60-Panel. Jurkat and MV4-11 cells were obtained from ATCC. OCI-AML5, RS4;11, SEM, ML-2, MOLM-13, MOLM14, NOMO-1, OCI-AML2, KOPN-8, EOL-1, and OCI-AML3 cells were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). All used cells were cultured in the appropriate media and conditions.

Brzezinka K., Nevedomskaya E., Lesche R., Steckel M., Eheim A.L., Haegebarth A, & Stresemann C. (2019). Functional diversity of inhibitors tackling the differentiation blockage of MLL-rearranged leukemia. Journal of Hematology & Oncology, 12, 66.

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Characterization of Human Leukemia Cell Lines

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RAW264.7, Raji, SK‐BR‐3, PC‐3, MM.1R (multiple myeloma), human AML MV4;11, RS4;11, TF‐1, Kasumi‐1, U‐937, THP‐1 and KG‐1a cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). Set‐2, EOL‐1 and Molm‐16 human AML cell lines were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (GmbH, Braunschweig, Germany). Mo7e AML cell line was provided by the Genetics Institute (Boston, MA, USA). All cell lines were maintained in RPMI‐1640 medium supplemented with 10% FBS at 37°C in a humidified 5% CO₂ atmosphere.

Xie C., He Y., Zhen M., Wang Y., Xu Y, & Lou L. (2017). Puquitinib, a novel orally available PI3Kδ inhibitor, exhibits potent antitumor efficacy against acute myeloid leukemia. Cancer Science, 108(7), 1476-1484.

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Hematological Cancer Cell Lines: Authentication and Mycoplasma Monitoring

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Human hematological cancer cell lines used in the study include EOL-1 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ], ACC-386), RS4;11 (ATCC, CRL-1873), MOLM-13 (DSMZ, ACC-554), K562 (ATCC, CRL-243) and MM1.S (ATCC, CRL-2974). These lines were cultured in the RPMI 1640 base medium supplemented with 10% of FBS and 1% of penicillin plus streptomycin. 293FT cells (Thermo Fisher Scientific, R70007) were cultured in DMEM base medium supplemented with 10% of FBS and 1% of penicillin plus streptomycin. Authentication of cell line identities was ensured by the Tissue Culture Facility (TCF) of UNC Lineberger Comprehensive Cancer Center with the genetic signature profiling and fingerprinting analysis. Every month, a routine examination for potential mycoplasma contamination was carried out by using the commercially available detection kits from Lonza.

Xu C., Meng F., Park K.S., Storey A.J., Gong W., Tsai Y.H., Gibson E., Byrum S.D., Li D., Edmondson R.D., Mackintosh S.G., Vedadi M., Cai L., Tackett A.J., Kaniskan H.Ü., Jin J, & Wang G.G. (2021). A NSD3-targeted PROTAC suppresses NSD3 and cMyc oncogenic nodes in cancer cells. Cell chemical biology, 29(3), 386-397.e9.

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CD30+ Hodgkin's Lymphoma Cell Line Protocol

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The CD30⁺ cHL cell lines L540, L428, KM-H2 and L1236, the CD30⁻ cHL cell line HD-MyZ and the acute myeloid (eosinophil) leukemia line EOL-1 were purchased from DSMZ (Braunschweig, Germany). The mast cell line HMC-1 was from Dr. J. Butterfield (Mayo Clinic, Rochester MN). Cells were cultivated in RPMI-1640 or IMDM (HMC-1) containing 10% FCS, penicillin, streptomycin and 50 μM β-mercaptoethanol. EV-depleted FCS was generated by overnight centrifugation at 100000 × g. The CD30 monoclonal antibodies Ki-2, Ki-3 were generated as described [38 (link), 44 , 45 (link)]. The goat anti-human Fc Ab (GaH-IgG) was from Dianova (Hamburg, Germany) and the metalloproteinase inhibitor (BB3644) was from British Biotech Pharmaceuticals Ltd. (Oxford, U.K.). The ADAM10 inhibitor GI254023X was kindly provided by Dr. A Ludwig, Aachen, Germany. The fluorescence resonance energy transfer metalloproteinase substrates PEPDAB010 and PEPMCA001 were from BioZyme Inc. (Apex, NC). Peripheral blood mononuclear cells were isolated from buffy coats from the local blood bank. The testing of plasma samples from cHL patients was approved by the local Ethics Committee (HD16 study, reference 09/039).

Hansen H.P., Trad A., Dams M., Zigrino P., Moss M., Tator M., Schön G., Grenzi P.C., Bachurski D., Aquino B., Dürkop H., Reiners K.S., von Bergwelt-Baildon M., Hallek M., Grötzinger J., Engert A., Leme A.F, & von Strandmann E.P. (2016). CD30 on extracellular vesicles from malignant Hodgkin cells supports damaging of CD30 ligand-expressing bystander cells with Brentuximab-Vedotin, in vitro. Oncotarget, 7(21), 30523-30535.

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Cell Lines for Cancer Research

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Human colorectal cancer cells HT29 and human breast cancer cells DU4475 were purchased from ECACC (Porton Down, UK). The human eosinophilic leukemia cell line EOL-1 was purchased from DSMZ (Braunschweig, Germany) and the human ovarian cancer cell line SKOV3 was from ATCC (Manassas, USA). HOS osteosarcoma cells, Molm13 acute myeloid leukemia cells and MV3 melanoma cells (all human) were kindly provided by the Departments of Pediatric Hematology and Oncology, Oncology and Dermatology, respectively (all University Medical Center Hamburg-Eppendorf, UKE). The human pancreatic cancer cell line PaCa5061 was provided by the Department of General, Visceral and Thoracic Surgery at UKE (Kalinina et al. 2010 (link)). The human gastric cancer cell line GC5023 was newly established in the framework of this study (see the next paragraph).
All aforementioned cell lines, except SKOV3, were grown in RPMI-1640 medium with 2 mM L-glutamine, supplemented with 10% fetal calf serum (FCS) and 1% penicillin (50 U/mL) and streptomycin (50 μg/mL) (all from Thermo Fisher Scientific, Waltham, USA), at 37°C with 95% H₂O-saturated atmosphere and 5% CO₂. SKOV3 cells were cultured in McCoy’s 5A medium containing 10% FCS, 2 mM L-glutamine and 1% penicillin/streptomycin (P/S) (all from Thermo Fisher Scientific).

Starzonek S., Maar H., Labitzky V., Wicklein D., Rossdam C., Buettner F.F., Wolters-Eisfeld G., Guengoer C., Wagener C., Schumacher U, & Lange T. (2020). Systematic analysis of the human tumor cell binding to human vs. murine E- and P-selectin under static vs. dynamic conditions. Glycobiology, 30(9), 695-709.

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Cell Line Characterization and Manipulation

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Cell lines used in the study included 293T (American Tissue Culture Collection [ATCC], CRL-3216), Hela (ATCC, CCL-2), MV4;11 (ATCC, CRL-9591), RS4;11 (ATCC, CRL-1873), MOLM-13 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ], ACC554), KOPN-8 (DSMZ, ACC552), EOL-1 (DSMZ, ACC386), K562 (ATCC, CRL-243), and THP-1 (ATCC, TIB-202). An MM1.S-derivative line with CRBN knockout (KO; CRBN-/-) was provided by Drs. J Brander and W Kaelin (Dana Farber Cancer Institute). Luciferase (luc)-labeled cells were generated by infection with MSCV-luciferase-IRES-neo retrovirus and subsequent selection. Luciferase expression was validated with Luciferase Assay System (Promega, #E1500). MV4;11 cells stably expressed with doxycycline (Dox)-inducible Cas9 (iCas9)⁶¹ were a gift of Drs. X Shi and H Wen (Van Andel Institute). Identity of cell lines was ensured by UNC’s Tissue Culture Facility with genetic signature analyses and examination of mycoplasma contamination performed using commercial detection kits (Lonza, #LT27–286).
Methods for cell transfection, generation of stable expression cell lines, assays for cell cycle progression, apoptosis and colony formation, as well as hematopoietic stem/progenitor cell (HSPC) purification and related colony formation unit (CFU) assay, were provided in Supplementary Note file.

Wang J., Yu X., Gong W., Liu X., Park K.S., Ma A., Tsai Y.H., Shen Y., Onikubo T., Pi W.C., Allison D.F., Liu J., Chen W.Y., Cai L., Roeder R.G., Jin J, & Greg Wang G. (2022). EZH2 non-canonically binds cMyc and p300 through a cryptic transactivation domain to mediate gene activation and promote oncogenesis. Nature cell biology, 24(3), 384-399.

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Cell Line Authentication and Maintenance

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U937, HL-60, MOLM13, UCSD-AML1, NB4, NOMO-1, and 293T cells were purchased from ATCC. CMK-86 cells were purchased from JCRB Cell Bank. EOL-1 were purchased from DSMZ. MV4–11, MOLM14 and THP-1 cells were provided by Scott Armstrong. MOLM13 and MOLM14 cells were originally derived from the same patient. All cell lines were maintained in RPMI 1640 (Cellgro) supplemented with 1% penicillin/streptomycin (PS; Cellgro) and 10% FBS (Sigma-Aldrich) at 37°C with 5% CO₂. The 293T cells were maintained in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% FBS (Invitrogen) and 1% PS. All cell lines were tested negative for Mycoplasma and were authenticated using short tandem repeat (STR) profiling.

Cremer A., Ellegast J.M., Alexe G., Frank E.S., Ross L., Chu S.H., Pikman Y., Robichaud A., Goodale A., Häupl B., Mohr S., Rao A.V., Walker A.R., Blachly J.S., Piccioni F., Armstrong S.A., Byrd J.C., Oellerich T, & Stegmaier K. (2019). Resistance mechanisms to SYK inhibition in acute myeloid leukemia. Cancer discovery, 10(2), 214-231.

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AML Cell Lines and Tyrosine Kinase Inhibitors

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Three AML cell lines MOLM-14, EOL-1, and MOLM-13 were obtained from DSMZ (Braunschweig, Germany) and HL-60 cells were purchased from Korean cell line bank (Seoul, Korea). Cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere with 5% CO2 at 37°C.
Gilteritinib (HY-12432), midostaurin (HY-10230), crenolanib (HY-13223), and quizartinib (HY-13001) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

Thái T.L, & Han S.Y. (2024). Gilteritinib Reduces FLT3 Expression in Acute Myeloid Leukemia Cells. Biomolecules & therapeutics.

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Cell Culture Maintenance and Characterization

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The human colorectal cancer cell line HT29 (purchased from the European Cell Culture Collection) and the human chronic eosinophilic leukemia cell line EOL-1 (from DSMZ) were maintained in RPMI-1640 supplemented with 2 mM L-glutamine, 10% fetal calf serum (FCS), 100 μg penicillin/mL and 100 μg streptomycin/mL (latter reagents were purchased from PAA) at 37°C in a humidified atmosphere of 5% CO₂. For all assays, HT29 cells were cultivated until 80% confluency; EOL-1 cells were grown in suspension. Primary human pulmonary microvascular endothelial cells (HPMECs) were obtained from Promocell, cultured in endothelial cell growth medium MV supplemented with the corresponding supplement mix as provided by the supplier, and 100 μg penicillin/mL and 100 μg streptomycin/mL. HPMECs were sub-cultured using a specific detach kit (PromoCell) according to the manufacturer’s instructions. All experiments with primary cells were performed during the first six passages.

Faryammanesh R., Lange T., Magbanua E., Haas S., Meyer C., Wicklein D., Schumacher U, & Hahn U. (2014). SDA, a DNA Aptamer Inhibiting E- and P-Selectin Mediated Adhesion of Cancer and Leukemia Cells, the First and Pivotal Step in Transendothelial Migration during Metastasis Formation. PLoS ONE, 9(4), e93173.

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Authentication and Characterization of AML Cell Lines

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The AML cell lines (EOL-1, HL-60, Kasumi-1, KG-1a, MOLM-13, MV4-11, NB-4, Nomo-1, SKM-1, THP-1, U937) were obtained from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) and American Type Culture Collection (Manassas, VA, USA). Routine testing for mycoplasma contamination was performed every 3 months, and the authenticity was determined by the flow cytometry-based immunophenotyping according to the provider’s description.

Stefańczyk S.A., Hagelstein I., Lutz M.S., Müller S., Holzmayer S.J., Jarjour G., Zekri L., Heitmann J.S., Salih H.R, & Märklin M. (2024). Induction of NK cell reactivity against acute myeloid leukemia by Fc-optimized CD276 (B7-H3) antibody. Blood Cancer Journal, 14(1), 67.

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