Paclitaxel (PTX) was obtained from JW Pharmaceutical Corporation (Dangjin, South Korea). Indocyanine green (ICG) and icosafluoro-15-crown-5-ether (PFC) were purchased from Tokyo Chemical Industry Co., LTD. (Tokyo, Japan). Chlorin e6 (Ce6) was supplied by Frontier Scientific (Salt Lake City, UT, USA). Bovine serum albumin (BSA) and 2-iminothiolane (2-IT; Traut’s reagent) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Four-arm polyethylene glycol maleimide (4-arm PEG-MAL; Mw 20 kDa) was purchased from NOF Corporation (Tokyo, Japan). The 4T1 breast cancer cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA). LIVE/DEAD™ viability/cytotoxicity assay kits, Singlet Oxygen Sensor Green reagent (SOSG), and CellROX™ Deep Red reagent were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-HIF-1α primary and Alexa Fluor®-488-conjugated goat anti-rabbit secondary antibodies were purchased from Abcam (Cambridge, MA, USA). Trypsin-EDTA and penicillin-streptomycin (P/S) solution were purchased from Corning (Somerville, NY, USA). Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from Capricorn (Ebsdorfergrund, Hesse, Germany). All other reagents were obtained from Sigma-Aldrich unless otherwise indicated.
Singlet oxygen sensor green reagent sosg
Manufactured by Thermo Fisher Scientific
Sourced in United States
Singlet Oxygen Sensor Green reagent (SOSG) is a fluorescent probe used to detect and measure the presence of singlet oxygen in biological and chemical systems. It functions by emitting a fluorescent signal upon reaction with singlet oxygen, enabling the quantification of this reactive oxygen species.
Automatically generated - may contain errors
Lab products found in correlation
Live dead viability cytotoxicity assay kit, by Thermo Fisher Scientific (2 mentions)
Anti hif 1α primary, by Abcam (2 mentions)
Alexa fluor 488 conjugated goat anti rabbit secondary antibody, by Abcam (2 mentions)
Penicillin streptomycin p s solution, by Corning (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Trypsin edta, by Corning (2 mentions)
Fetal bovine serum (fbs), by Capricorn (2 mentions)
Chlorin e6 ce6, by Frontier Scientific (2 mentions)
4t1 breast cancer cell, by American Type Culture Collection (2 mentions)
Ammonium hydroxide solution, by Merck Group (2 mentions)
Oleic acid, by Merck Group (2 mentions)
1 octadecene, by Merck Group (2 mentions)
Cellrox deep red reagent, by Thermo Fisher Scientific (1 mentions)
Indocyanine green icg, by Tokyo Chemical Industry (1 mentions)
Dulbecco s modified eagle medium dmem, by Capricorn (1 mentions)
Kerosene, by Merck Group (1 mentions)
Cellrox green reagent, by Thermo Fisher Scientific (1 mentions)
1 plus kit, by Hypoxyprobe (1 mentions)
Penicillin streptomycin solution, by Macgene (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
4 protocols using singlet oxygen sensor green reagent sosg
1
Photodynamic Therapy Nanoparticle Formulation
2
Multifunctional Theranostic Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Erbium acetate hydrate (III), lutetium acetate hydrate (III), ammonium fluoride (NH4F), oleic acid, 1-octadecene, ammonium hydroxide solution, polyacrylic acid (Mw 1,800), hemoglobin (Hb; bovine), bovine serum albumin (BSA), and kerosene were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chlorin e6 (Ce6) was purchased from Frontier Scientific (Salt Lake City, UT, USA). Icosafluoro-15-crown-5-ether (PFC) was purchased from Tokyo Chemical Industry Co., LTD. (Tokyo, Japan). SY-Glyster CR-310 was kindly provided by Sakamoto Yakuhin Kogyo Co. (Osaka, Japan). 4T1 breast cancer cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA). LIVE/DEAD™ viability/cytotoxicity assay kits, singlet oxygen sensor green reagent (SOSG), and CellROX™ Green reagent were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti–HIF–1α primary and Alexa Fluor®-488-conjugated goat anti-rabbit secondary antibodies were purchased from Abcam (Cambridge, MA, USA). In situ cell death detection (terminal deoxynucleotidyl transferase dUTP nick end labeling: TUNEL) assay kits were purchased from Roche Diagnostics GmbH (Mannheim, Germany). Hypoxyprobe™-1 Plus kits were obtained from Hypoxyprobe Inc. (Burlington, MA, USA). All other reagents were obtained from Sigma-Aldrich unless otherwise indicated.
3
Multifunctional Nanoparticle-based Phototherapies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zinc phthalocyanine (ZnPc, Strem Chemicals, Inc., American) and single-walled carbon nanohorns (SWCNH, Qingdajiguang Co. Ltd., China) were used as PDT/PTT agent, respectively. D -α-tocopherol polyethylene glycol 1000 succinate (TPGS, Aladdin Co. Ltd., China) were selected as a stabilizer. Dimethyl sulfoxide (DMSO, Sinopharm Chemical Reagent Beijing Co. Ltd., China) and ethanol (Eth, Sinopharm Chemical Reagent Beijing Co. Ltd., China) of analytical grade were added as solvents to promote ZnPc and SWCNH adsorption. Singlet oxygen sensor green reagent (SOSG, Thermo Fisher Co. Ltd., American) was used to detect singlet oxygen concentration after PDT therapy. Cell Counting Kit-8 (CCK-8, Beyotime Biotechnology Co. Ltd., China) was used to investigate cell viability after therapy. Tribromoethanol (Energy Chemical Co. Ltd., China) was used as an anesthetic for mice. Fetal bovine serum (FBS, PAN Biotech Co. Ltd., Germany), dulbecco's modified eagle medium (DMEM, Macgene Co. Ltd., China), penicillin-streptomycin solution (Macgene Co. Ltd., China), and trypsin (Macgene Co. Ltd., China) were used for cell culture.
4
Synthesis of Photosensitizer-Loaded Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Erbium(III) acetate hydrate, lutetium(III) acetate hydrate, yttrium(III) acetate hydrate, yttErbium(III) acetate hydrate, neodymium(III) acetate hydrate, ammonium fluoride (NH4F), oleic acid (90%), 1-octadecene (90%), IGEPAL® CO-520, and ammonium hydroxide solution (28–30%) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Tetraethyl orthosilicate (TEOS), 3-aminopropyltriethoxy-silane (APTES), and tetrakis(4-carboxyphenyl)porphyrin (TCPP) were purchased from Tokyo Chemical Industry (TCI) Co., Ltd. (Toshima, Kita-ku, Tokyo, Japan). mPEG2k-SC and FA/PEG5k-NHS were purchased from Biochempeg (Watertown, MA, USA). The HeLa (ATCC® CCL-2™) human adenocarcinoma cell line was obtained from the American Type Culture Collection (Rockville, MD, USA). DMEM and fetal bovine serum (FBS) were purchased from Capricorn (Ebsdorfergrund, Hesse, Germany). Trypsin-EDTA and penicillin-streptomycin (P/S) solution were purchased from Corning (Corning, NY, USA). The LIVE/DEADTM viability/cytotoxicity assay kit, LysoTrackerTM Green DND-26, and singlet oxygen sensor green reagent (SOSG) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were obtained from Sigma–Aldrich unless otherwise indicated.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!