Mouse anti ccnd1

Manufactured by Santa Cruz Biotechnology

The Mouse anti-CCND1 is a primary antibody that specifically recognizes the Cyclin D1 (CCND1) protein. CCND1 is a key regulator of the cell cycle progression and is involved in the transition from the G1 phase to the S phase. This antibody can be used in various applications such as Western blotting, immunohistochemistry, and flow cytometry to detect and study the expression of CCND1 in biological samples.

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CCND1 (16 citations) Anti-CCND1 (9 citations)

3 protocols using mouse anti ccnd1

Immunoblot and Immunohistochemistry Analysis

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Immunoblot analysis and IHC was performed as previously described [32 (link)], using rabbit anti-TFF3, rabbit anti-pSTAT3, and mouse anti-STAT3 was obtained from Abcam, Cambridge, MA. While the mouse anti-CCND1, mouse anti-BCL2, mouse anti-β-ACTIN antibodies were obtained from Santa Cruz Biotechnology, CA.

Pandey V., Zhang M., Chong Q.Y., You M., Raquib A.R., Pandey A.K., Liu D.X., Liu L., Ma L., Jha S., Wu Z.S., Zhu T, & Lobie P.E. (2017). Hypomethylation associated enhanced transcription of trefoil factor-3 mediates tamoxifen-stimulated oncogenicity of ER+ endometrial carcinoma cells. Oncotarget, 8(44), 77268-77291.

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Western Blot Analysis of Cell Signaling

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Immunoblot analysis was performed
as previously described^{58 (link)−60 (link)} using mouse anti-β-Actin,
mouse anti-p-ERK, rabbit anti-ERK, mouse anti-p-EGFR (Y1045), mouse
anti-p-EGFR (Y1148), mouse anti-p-EGFR (Y1173), mouse anti-CCND1,
mouse anti-CDK4, mouse anti-CCNB1, rabbit anti-c-MYC, mouse anti-BCL2,
mouse anti-BCL-XL, mouse anti-BAD, rabbit anti-CYCS, mouse anti-p-ERK
(44/42), and rabbit anti-ERK antibodies were procured from Santa Cruz
Biotechnology, CA. Mouse anti-CDH1, mouse anti-CDH2, rabbit anti-OCLN,
rabbit anti-pSTAT3, and mouse anti-STAT3 antibodies were obtained
from Abcam, Cambridge, MA. Rabbit anti-p-EGFR (Y992) and rabbit anti-p-CDK2
antibodies were obtained from Cell Signaling. Cell extracts were resolved
on SDS-PAGE and immunoblotted, with the appropriate and respective
antibodies. β-Actin was used as input control for cell lysate.
The sizes of the detected protein bands are shown in kilodaltons on
the left side.

Sebastian A., Pandey V., Mohan C.D., Chia Y.T., Rangappa S., Mathai J., Baburajeev C.P., Paricharak S., Mervin L.H., Bulusu K.C., Fuchs J.E., Bender A., Yamada S., B.a.s.a.p.p.a., Lobie P.E, & Rangappa K.S. (2016). Novel Adamantanyl-Based Thiadiazolyl Pyrazoles Targeting EGFR in Triple-Negative Breast Cancer. ACS Omega, 1(6), 1412-1424.

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Immunoblot and Confocal Microscopy Analysis

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Immunoblot analysis was performed as previously described^{8 (link),35 (link)}, using rabbit anti-TFF3 polyclonal antibody. Mouse anti-β-actin, mouse anti-CDKN1A, mouse anti-BCL2, mouse anti-CDK2, rabbit anti-CDK4, rabbit anti-CCNE1, mouse anti-cSRC, and mouse anti-CCND1 antibody was obtained from Santa Cruz Biotechnology, CA. Rabbit anti-p-cSRC antibody were obtained from Cell Signaling, USA. Rabbit anti-pSTAT3(Tyr705) and mouse anti-STAT3 antibodies were obtained from Abcam, Cambridge, MA. Confocal microscopy scanning was performed as previously described^{51 (link)}. Secondary antibody, Alexa Fluor 488 goat anti-rabbit IgG was purchased from Invitrogen, Singapore. Rhodamine-conjugated phalloidin (Sigma, St Louis, MO) was used to visualize f-actin filaments.

Pandey V., Zhang M., You M., Zhang W., Chen R., Zhang W., Ma L., Wu Z.S., Zhu T., Xu X.Q, & Lobie P.E. (2018). Expression of two non-mutated genetic elements is sufficient to stimulate oncogenic transformation of human mammary epithelial cells. Cell Death & Disease, 9(12), 1147.

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