Goat anti mouse alexafluor 488 a11001

Rabbit anti-pT202/Y204 ERK1/2 (#9101S) and rabbit anti-pY416 SFK (#2101S) were obtained from Cell Signaling, Beverly, MA, USA. Rabbit anti-pY466 cortactin (PA114115) was obtained from Thermo Scientific, Waltham, MA, USA. Mouse monoclonal anti-β-actin (AC-74, A2228) was obtained from Sigma-Aldrich. Mouse monoclonal anti-cortactin (A-4; sc-55578) was from Santa Cruz Biotechnology (Santa Cruz County, CA, USA). The secondary antibody, goat anti-mouse AlexaFluor® 488 (A11001), was from Invitrogen, Carlsbad, CA, USA. Goat anti-rabbit IgG peroxidase (A6154) and goat anti-mouse IgG peroxidase (A4416) were obtained from Sigma–Aldrich.

Propidium iodide and DABCO were obtained from Sigma-Aldrich (USA). Quant-iT PicoGreen dsDNA Assay Kit, Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit, and PrestoBlue Cell Viability Reagent were acquired from Invitrogen (Thermo Fisher Scientific, USA).
We used the following pharmacological inhibitors/inducers in the study: phorbol 12-myristate 13-acetate (PMA; 100 nM), cytochalasin D (CytD; 10 µg/mL), PD98059 (PD98; MEK inhibitor; 60 µM), and 3-Methyladenine (3-MA; class III PI3K Vps34 and autophagy inhibitor; 5 mM) from Sigma-Aldrich; neutrophil elastase inhibitor III (NEi; MeOSuc-AAPV-CMK; 10 µg/mL), myeloperoxidase inhibitor I (MPOi; 600 nM), and BAPTA-AM (BAPTA; [Ca²⁺]i chelator; 10 µM) from Calbiochem (USA); IC87114 (IC87; selective inhibitor of PI3Kδ; 1 µM) and chloroamidine (Cl-A; PAD inhibitor; 12 μM) from Cayman Chemical (USA); and AS605240 (AS60; selective inhibitor of PI3Kγ; 10 µM) from Tocris Bioscience (UK).
The following antibodies were used in this study: mouse anti-histone H1 monoclonal (sc-8030; 1:100) from Santa Cruz Biotechnology (USA); rabbit anti-myeloperoxidase polyclonal (PA5-16672; 1:200), mouse anti-toxoplasma monoclonal (MA1-83499; 1:100), Goat anti-Mouse Alexa Fluor 488 (A11001; 1:2000), Goat anti-Mouse Alexa Fluor 546 (A11003; 1:400), and Goat anti-Rabbit Alexa Fluor 594 (A11037; 1:800) from Invitrogen.