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C57bl 6 h 2b

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C57BL/6 (H-2b) is a mouse strain that expresses the H-2b major histocompatibility complex (MHC) haplotype. This strain is widely used in immunology research and as a model for various diseases and experimental procedures.

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Lab products found in correlation

Balb c h 2d, by Jackson ImmunoResearch (13 mentions) 129 svj h 2b wt mice, by Jackson ImmunoResearch (2 mentions) Penicillin, by Corning (1 mentions) Streptomycin, by Corning (1 mentions) Puromycin selection, by InvivoGen (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Corning (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) C3h sw h2b, by Jackson ImmunoResearch (1 mentions) Balb c h 2d mice, by Jackson ImmunoResearch (1 mentions) Cd4 tgfbr2, by Jackson ImmunoResearch (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) B6.129 tnfrsf1atm1mak j, by Jackson ImmunoResearch (1 mentions) B6.129s2 tn frsf1btm1mwm j, by Jackson ImmunoResearch (1 mentions) Tyr creer, by Jackson ImmunoResearch (1 mentions) Drosophila expression system des, by Thermo Fisher Scientific (1 mentions) B6 rag1 h 2b, by Jackson ImmunoResearch (1 mentions) Ccr6 h 2b, by Jackson ImmunoResearch (1 mentions) Fvb n h 2q, by Taconic Biosciences (1 mentions)

25 protocols using c57bl 6 h 2b

1

Murine Autoimmune Disease Models

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NOD/LtJ (H-2g7), NZBW(H-2d/z), MRL (H-2k), and C57BL/6 (H-2b) mice were purchased from the Jackson Laboratories (Bar Harbor, Maine). All mice were housed under specific pathogen-free barrier conditions and were analyzed at 8–11 weeks of age, prior to the onset of clinical disease symptoms.
Elizer S.K., Marshall A.F, & Moore D.J. (2014). Dysregulation of T Lymphocyte Proliferative Responses in Autoimmunity. PLoS ONE, 9(8), e106347.
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2

IFNAR Knockout Mice for ZIKV Study

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Experiments involving animals were performed under Cincinnati Children’s Hospital Institutional Animal Care and Use Committee (IACUC) approved protocols (Assurance Number 2018–0022). IFNAR−/− mice on the C57BL/6 (H-2b) background, isogenic C57BL/6 control mice, and Balb/c (H-2d) were purchased were obtained from The Jackson Laboratory, and bred in our facility. Mice between 6–12 weeks of age, with gender and sex matched Lm-ZIKV and Lm-HSV primed groups, were used in each experiment. For neutralizing IFNAR, IFNAR+/+ were intraperitoneally administered anti-IFNAR antibody (MAR1–5A3; BE0241; BioXcell, West Lebanon, New Hampshire) beginning 1 day prior (0.4 mg/mouse), and days +1 (0.2 mg/mouse), +3 (0.2 mg/mouse), +5 (0.2 mg/mouse) after MR766 ZIKV challenge. Male mice on the Balb/c (H-2d) background were used to sire allogeneic pregnancies in IFNAR−/− female mice.
Burg A.R., Erickson J.J., Turner L.H., Pham G., Kinder J.M, & Way S.S. (2020). Persistent Zika virus clinical susceptibility despite reduced viral burden in mice with expanded virus-specific CD8+ T cells primed by recombinant Listeria monocytogenes. Journal of immunology (Baltimore, Md. : 1950), 205(2), 447-453.
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3

Mouse Strain Comparison for Pathogen-free Study

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Male C57BL/6 (H-2b) and BALB/c (H-2d) mice were purchased from the Jackson Laboratory (Bar Harbor, ME). All mice were maintained under specific pathogen-free conditions. Animal use and care conformed to the guidelines established by the American Association for Accreditation of Laboratory Animal Care and approved by the institutional animal care committee at Houston Methodist Hospital in Houston, Texas.
Wu C., Zhao Y., Xiao X., Fan Y., Kloc M., Liu W., Ghobrial R.M., Lan P., He X, & Li X.C. (2016). Graft-Infiltrating Macrophages Adopt an M2 Phenotype and Are Inhibited by Purinergic Receptor P2X7 Antagonist in Chronic Rejection. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(9), 2563-2573.
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4

Establishment of Genetically Modified Mice

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C57BL/6 (H-2b) and 129/SvJ (H-2b) WT mice were obtained from the Jackson Laboratory. BALB/c (H-2d) mice were purchased from NCI and Charles River Laboratory. GzmB−/− mice in the C57BL/6 strain and 129/SvJ strain were developed and maintained as previously described [12 (link),14 (link),15 (link)]. A20 lymphoma cells, derived from BALB/c strain, were transduced to express luciferase as previously described and used for bioluminescence imaging to measure tumor burden in vivo [12 (link),14 (link),16 ,17 (link)]. All mice were maintained in SPF housing, and all experiments were conducted in accordance with the animal care guidelines at Roswell Park Cancer Institute, using protocols approved by animal studies committee.
Du W., Leigh N.D., Bian G., Alqassim E., O'Neill R.E., Mei L., Qiu J., Liu H., McCarthy P.L, & Cao X. (2016). Granzyme B Contributes to the Optimal Graft-Versus-Tumor Effect Mediated by Conventional CD4+ T Cells. Journal of immunology research and therapy, 1(1), 22-28.
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5

Generating MHCI-deficient Cell Line

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Six-week-old to 8-week-old C57BL/6 (H-2b) and BALB/c (H-2d) mice were purchased from Jackson Laboratory (Bar Harbor, Maine). TC1 (H-2b), CT26 (H-2d), and H5V (H-2b) cells were cultured in RPMI 1640 (Cellgro; #10-104-CV) medium supplemented with 2 mM l-glutamine (Gibco, #35 050-061) and 150 U/mL streptomycin plus 200 U/mL penicillin (Cellgro; #30-0010 CI) and 10% heat-inactivated FBS (Life Technologies, #16000044). All cell lines were obtained from ATCC and used within 15–20 passages. The CT26 MHCI-deficient cell line was obtained by knockdown of the beta-2 microglobulin gene (B2M) using “TRC lentiviral shRNA technology” (Dharmacon; RMM4534-EG12010). Puromycin selection (10 µg/mL; InvivoGen; #ant-pr-1) was used to select for cells containing the plasmid, and cell sorting was used to generate single cell population.
Pierini S., Mishra A., Perales-Linares R., Uribe-Herranz M., Beghi S., Giglio A., Pustylnikov S., Costabile F., Rafail S., Amici A., Facciponte J.G., Koumenis C, & Facciabene A. (2021). Combination of vasculature targeting, hypofractionated radiotherapy, and immune checkpoint inhibitor elicits potent antitumor immune response and blocks tumor progression. Journal for Immunotherapy of Cancer, 9(2), e001636.
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6

Establishment of Genetically Modified Mice

Cited 1 time
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C57BL/6 (H-2b) and 129/SvJ (H-2b) WT mice were obtained from the Jackson Laboratory. BALB/c (H-2d) mice were purchased from NCI and Charles River Laboratory. GzmB−/− mice in the C57BL/6 strain and 129/SvJ strain were developed and maintained as previously described [12 (link),14 (link),15 (link)]. A20 lymphoma cells, derived from BALB/c strain, were transduced to express luciferase as previously described and used for bioluminescence imaging to measure tumor burden in vivo [12 (link),14 (link),16 ,17 (link)]. All mice were maintained in SPF housing, and all experiments were conducted in accordance with the animal care guidelines at Roswell Park Cancer Institute, using protocols approved by animal studies committee.
Du W., Leigh N.D., Bian G., Alqassim E., O'Neill R.E., Mei L., Qiu J., Liu H., McCarthy P.L, & Cao X. (2016). Granzyme B Contributes to the Optimal Graft-Versus-Tumor Effect Mediated by Conventional CD4+ T Cells. Journal of immunology research and therapy, 1(1), 22-28.
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7

Murine F1 Pups for Neonatal Vaccine Development

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For all experiments we used murine F1 pups (H-2b×H-2d) derived from crosses between C57BL/6 (H-2b) and C57B10.D2 (H-2d) mice provided from the Jackson Laboratories (Bar Harbor, ME,USA) to match our previous work on the development of our neonatal vaccine platform based on Lm Δ(trpS actA)/pSP0-PShlyOVA (LmOVA).19 (link) F1 mice were vaccinated either 6 days post-partum (Fig. 1 and 4). All animals were maintained under pathogen-free conditions at the Child and Family Research Institute of the University of British Columbia according to animal experiment protocols approved by the Institutional Animal Care and Use Committee. When analyzing the asthma model, we did not detect any difference between males and females for any of the parameters tested, and thus data presented were derived from both genders combined unless specified. We did, however, detect a significantly higher response in female vs. male mice to Saccharopolyspora rectivirgula (SR)-antigen in the HP model for all parameters tested and thus data presented were obtained using female mice only in this model.
Aloyouni S.Y., Segeritz C.P., Sherrid A.M., Gold M.J., Loeffler D.I., Blanchet M.R., Cai B., Hirota J., McNagny K.M, & Kollmann T.R. (2014). Perinatal Immunization With Vaccine-Grade Listeria monocytogenes Provides Protection Against Murine Th2 Airway Inflammation. Allergy, Asthma & Immunology Research, 6(4), 341-349.
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8

Allogeneic Bone Marrow Transplant

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Female donor C3H.SW (H2b) and recipient C57BL/6 (H2b) mice were purchased from Jackson Laboratory (Bar Harbor, ME). Animals were kept in pathogen-free conditions and used at 8 to 10 weeks of age. All mice were maintained at the UC Davis Medical Center vivarium in accordance with Institutional Animal Care and Use Committee (IACUC) standards.
Pai C.C., Hsiao H.H., Sun K., Chen M., Hagino T., Tellez J., Mall C., Blazar B.R., Monjazeb A., Abedi M, & Murphy W.J. (2014). Therapeutic benefit of bortezomib on acute GVHD is tissue specific and is associated with IL-6 levels. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 20(12), 1899-1904.
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9

Helminth Infection Model in Mice

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We utilized wild type (WT) C57BL/6 (H2b) and Balb/C (H2d) mice (The Jackson Laboratory, Bar Harbor, ME) as well as a C57BL/6 mouse strain with a T cell-specific defect in TGFβ signaling (Cd4-TGFBR2 (Jackson Laboratories #005551; also named TGFβ receptor II dominant negative (TGFβ RII DN)) (H2b)(29 (link)). Five to six week old Balb/C mice were inoculated with 150 H. polygyrus third stage larvae (L3) by oral gavage. Infective H. polygyrus L3 (original specimens archived at the U.S. National Helminthological Collection no. 81930; also named H. polygyrus (bakeri) or H. bakeri in some publications (30 (link), 31 (link)) were obtained from mouse fecal cultures of eggs by the modified Baermann method and stored at 4°C until used. The number of eggs in hydrated stool pellets was enumerated in duplicate at the indicated time points for each mouse and displayed as egg number/stool weight. Mice were housed and handled following national guidelines and as approved by our Animal Review Committee.
Li Y., Chen H.L., Bannick N., Henry M., Holm A.N., Metwali A., Urban JF J.r., Rothman P.B., Weiner G.J., Blazar B.R., Elliott D.E, & Ince M.N. (2014). Intestinal Helminths Regulate Lethal Acute Graft Versus Host Disease and Preserve Graft Versus Tumor Effect in Mice. Journal of immunology (Baltimore, Md. : 1950), 194(3), 1011-1020.
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10

Generation of Luciferase-Expressing Lag-3 Knockout Mice

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C57BL/6 (H-2b) and Balb/c (H-2d) mice were purchased from Jackson Laboratory. Lag-3−/− mice were a gift from Yueh-Hsiu Chien (Stanford University). Luciferase expressing (luc+) C57BL/6 mice (H-2b, CD45.1+, Thy1.1+) were created as previously described [6] (link). Luc+Lag-3−/− mice were generated by breeding Lag-3−/− mice and luc+ C57BL/6 mice for three generations.
Sega E.I., Leveson-Gower D.B., Florek M., Schneidawind D., Luong R.H, & Negrin R.S. (2014). Role of Lymphocyte Activation Gene-3 (Lag-3) in Conventional and Regulatory T Cell Function in Allogeneic Transplantation. PLoS ONE, 9(1), e86551.
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