Escherichia colitop 10

Manufactured by Takara Bio

Sourced in Japan

Escherichia coliTOP 10 is a strain of E. coli bacteria commonly used in molecular biology laboratories. It is a competent strain, meaning it can efficiently take up and maintain plasmid DNA. The strain is designed for high-efficiency transformation and reliable plasmid propagation.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using escherichia colitop 10

Sourcing Chemicals for Fungal BGL1 Cloning

Check if the same lab product or an alternative is used in the 5 most similar protocols

Salicin was bought from Sigma-Aldrich (St. Louis, MO, USA). Furfural and 5-hydroxymethyl Furfural (5-HMF) were purchased from Acros Organics (NJ, USA). Vanillin, 4-hydroxybenzaldehyde, sodium formate, sodium acetate, sodium hydroxide, KCl and NaCl were purchased from Sinopharm Chemical Reagents (Shanghai, China). Formic acid, acetic acid and levulinic acid were purchased from local chemical reagent companies in Nanchang, China. The Glucose Assay Kit and zeocin were bought from Robio, China, and Invitrogen, USA, respectively.
P. oxalicum 16 (PO16) for BGL1 gene cloning was isolated from the local soil of Nanchang [23 (link)]. Escherichia coliTOP 10 was purchased from Takara, Japan. pGAPZαA for constitutive expression of P. pastoris GS115 was purchased from Invitrogen, USA.

Li H., Yi S., Bell E.W., Huang Q, & Zhao X. (2019). Recombinant Penicillium oxalicum 16 β-glucosidase 1 displays comprehensive inhibitory resistance to several lignocellulose pretreatment products, ethanol, and salt. Applied biochemistry and biotechnology, 191(2), 772-784.

+ Open protocol

+ Expand

Cloning and Expression in Pichia pastoris

Check if the same lab product or an alternative is used in the 5 most similar protocols

Escherichia coli TOP10, plasmid pPICZαA, T4 DNA ligase and DNA polymerase were purchased from Takara Biotechnology (Dalian). Pichia pastoris X-33 was stored in our laboratory. Beta-chitin was purchased from Sigma (St. Louis, MO, USA). Alpha-chitin was purchased from Seikagaku (Tokyo, Japan) [33 (link)]. Colloidal chitin was prepared according to the previously reported method [48 ]. AMAC, chitin oligosaccharides (GlcNAc) n (n = 2–6, dimers to hexamers (A₂ to A₆; A, GlcNAc)) were purchased from Sigma-Aldrich (Munich, Germany). Unless otherwise noted, all reagents were analytical grade. Acetate release was measured using an acetate kit from R-Biopharm (Darmstadt, Germany) [14 (link)].

Zhu X.Y., Zhao Y., Zhang H.D., Wang W.X., Cong H.H, & Yin H. (2019). Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides. Marine Drugs, 17(2), 74.

+ Open protocol

+ Expand

Recombinant Xylanase Production in P. pastoris

Check if the same lab product or an alternative is used in the 5 most similar protocols

Escherichia
coli Top10 and the pMD18-T vector used for gene cloning
and sequencing were obtained from Takara (Otsu, Japan). P. pastoris X-33 and vector pPICZα-C were used
for gene expression and were purchased from Invitrogen (Carlsbad,
CA, USA). Kits for fungal DNA extraction, DNA purification, and plasmid
isolation were purchased from Omega (Norcross, GA, USA). Restriction
endonucleases, T4 DNA ligase, DNA polymerase, dNTPs, and zeocin were
purchased from Thermo Fisher Scientific (Ipswich, MA, USA). Nickel-NTA
agarose (Qiagen, Valencia, CA, USA) was used to purify the His₆-tagged protein. An RNeasy Plant Mini Kit (Qiagen, Valencia,
CA, USA) was used to extract total RNA. A TransScript One-Step gDNA
Removal and cDNA Synthesis SuperMix kit (TransGen Biotech, Beijing,
China) was used for first-strand cDNA synthesis. Beechwood xylan substrate,
yeast nitrogen base (YNB) medium, and biotin were purchased from Sigma
(St. Louis, MO, USA). Corncob xylan was purchased from Yuanye Biotech
(Shanghai, China). All other chemicals were of analytical grade and
commercially available.

Wu J., Qiu C., Ren Y., Yan R., Ye X, & Wang G. (2018). Novel Salt-Tolerant Xylanase from a Mangrove-Isolated Fungus Phoma sp. MF13 and Its Application in Chinese Steamed Bread. ACS Omega, 3(4), 3708-3716.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!