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Anti mouse igg1 isotype control clone mopc 21

Manufactured by BioXCell
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Anti-mouse IgG1 isotype control (clone MOPC-21) is a laboratory reagent used as a control in immunological experiments involving mouse IgG1 antibodies. It serves as a negative control to help distinguish specific antigen-antibody interactions from non-specific binding.

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Lab products found in correlation

Pe conjugated anti human cd11b, by Thermo Fisher Scientific (1 mentions) Recombinant human tnf α, by R&D Systems (1 mentions) Tgf β1, by R&D Systems (1 mentions)

Close spelling variants of this product

Mouse IgG1 isotype control (clone MOPC-21, Isotype control (clone MOPC-21) Mouse IgG1 (clone MOPC-21; Mouse IgG1 (MOPC-21), Mouse IgG1 isotype control IgG1 (MOPC-21; Clone MOPC-21 IgG1 isotype control MOPC-21 Isotype control

2 protocols using anti mouse igg1 isotype control clone mopc 21

1

Activation of Endothelial Cell Markers

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Recombinant human TNFα, TGF-β1, and latent TGF-β1 protein were purchased from R&D Systems (Minneapolis, MN, USA). Anti-mouse TGF-β (clone 1D11.16.8) and anti-mouse IgG1 isotype control (clone MOPC-21) were purchased from Bio X Cell (West Lebanon, NH, USA). PercP-Cy5.5-conjugated mouse anti-human CD66b (clone G10F5), FITC-conjugated mouse anti-human CD11a (clone HI111), PE-conjugated mouse anti-human CD11b (clone ICRF44), PE-conjugated mouse anti-human CD62E (clone 68-5H11), and FITC-conjugated mouse anti-human CD106 (VCAM-1, clone 51-10C9) were purchased from BioLegend (San Diego, CA, USA). PE-conjugated anti-human CD11b (activation epitope-specific antibody, clone CBRM1/5), APC-conjugated CD62P monoclonal antibody (P-selectin, clone Psel.K02.3), and PERCP-conjugated ICAM-1 monoclonal antibody (clone 1H4) were purchased from eBioscience (Thermo Fisher Scientific, Waltham, MA, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated.
Torres L.S., Chweih H., Fabris F.C., Gotardo E.M., Leonardo F.C., Saad S.T., Costa F.F, & Conran N. (2022). TGF-β1 Reduces Neutrophil Adhesion and Prevents Acute Vaso-Occlusive Processes in Sickle Cell Disease Mice. Cells, 11(7), 1200.
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2

IFNAR Blockade in LCMV Infection

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WT mice were treated with 500μg/mouse intraperitoneally (i.p.) with neutralizing antibody (nAb) to IFNAR (clone MAR1-5A3; BioXCell) or Anti-Mouse IgG1 Isotype Control (clone MOPC-21; BioXCell) on days -1 and 0 post-LCMV Cl13-infection. Mice were then treated with 250μg/mouse anti-IFNAR nAb or isotype control i.p. on days 2, 4, and 6 post-LCMV Cl13 infection as previously described (Wilson et al., 2013 (link)). Uninfected control mice were similarly treated in parallel.
Macal M., Jo Y., Dallari S., Chang A.Y., Dai J., Swaminathan S., Wehrens E., Fitzgerald-Bocarsly P, & Zúñiga E.I. (2018). Self-renewal and Toll-like receptor Signaling Sustain Exhausted Plasmacytoid Dendritic Cells During Chronic Viral Infection. Immunity, 48(4), 730-744.e5.
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