Macrogol 15 hydroxystearate

Compounds were firstly dissolved with macrogol 15 hydroxystearate (a nonionic solubilizer [BASF, Ludwigshafen, Germany]) for injection with or without medium chain triglycerides (MCT), from BASF, in a water bath at 37°C. The concentration of compounds was 2 mg/mL. The concentration of solubilizer ranged from 5%–10%, and MCT 0.5%–2% in the final solution. For the vehicle, the mixture of solubilizer and MCT was prepared at 10% and 2%, respectively. Male C57BL/6 mice weighing 18–22 g were pretreated with compound S1 or S4 (10 mg/kg) in a water solution by intravenous injection 15 minutes before the intraperitoneal injection of LPS (15 mg/kg). Control animals received a similar volume (200 μL) of vehicle. Body weight change and mortality were recorded for 7 days.

Male C57BL/6 mice were obtained from the Animal Center of Wenzhou Medical College (Wenzhou, People’s Republic of China). Animals were housed at a constant room temperature, with a 12/12-hour light–dark cycle, and fed with a standard rodent diet and water. The animals were acclimatized to the laboratory for at least 7 days before use in the experiments. Protocols involving the use of animals were approved by the Wenzhou Medical College Animal Policy and Welfare Committee (Approval documents: 2009/APWC/0031). Compound 7i was firstly dissolved with macrogol 15 hydroxystearate (a nonionic solubilizer for injection, from BASF [Ludwigshafen, Germany]) with or without medium-chain triglycerides (MCT, from BASF) in a water bath at 37°C. The concentration of 7i was 2 mg/mL. The concentration of solubilizer was 5%–10%, and MCT 0.5%–2.0% in final solution. For the vehicle, the mixture of solubilizer and MCT was prepared at 10% and 2%, respectively. Male C57BL/6 mice weighing 18–22 g were treated with 7i solution (200 μL, 15 mg/kg) by intravenous injection 15 minutes before the intraperitoneal injection of LPS (25 mg/kg). Control animals received a similar volume (200 μL) of vehicle. Bodyweight change and mortality were recorded for 7 days.