Goat anti rabbit af488

Manufactured by Abcam

Sourced in United Kingdom

Goat anti-rabbit AF488 is a secondary antibody conjugated with the Alexa Fluor 488 fluorescent dye. It is designed to detect and bind to rabbit primary antibodies, allowing for fluorescent labeling and visualization of target proteins or antigens in various applications such as immunohistochemistry, flow cytometry, and Western blotting.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti rabbit cy3, by Thermo Fisher Scientific (1 mentions) Fatp2, by Abcam (1 mentions) Cd4 pecf594 rm4 5, by BD (1 mentions) Ki67 b56, by BD (1 mentions) Fibronectin, by Merck Group (1 mentions) 7 aad, by BD (1 mentions) Rabbit anti mouse cd3 antibody, by Abcam (1 mentions) Anti fluorescence quenching agent, by Wuhan Servicebio Technology (1 mentions) Egm 2 medium, by Lonza (1 mentions) Goat anti mouse af488, by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal anti pv, by Swant (1 mentions) A1 hd25 confocal microscope, by Nikon (1 mentions) Vectashield antifade reagent with dapi, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Rabbit anti- (5 citations) Goat anti-rabbit IgG H&L-AF488 (2 citations)

4 protocols using goat anti rabbit af488

Flow Cytometric Analysis of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometric analysis was performed using the following antibodies: CD4 PECF594 (RM4‐5) from BD Biosciences and CD8 PerCP (SK1), CD45RA BV605 (HI100), CD27 BV421 (O323), CD28 BV785 (CD28.2), CCR7 PECy7 (G043H7) and CD36 APCCy7 (5‐271) from BioLegend. FATP2 (Abcam) and FATP3 (Atlas antibodies) were measured in conjunction with goat anti‐rabbit AF488 (Abcam). Cortactin expression was assessed using rabbit anti‐human cortactin antibody (PA5‐27134; Life Technologies) stained in conjugation with goat anti‐rabbit Cy3 (Life Technologies). PGC1α (3G6) and p‐p53 (16G8) both from Cell Signaling, and Ki67 (B56; BD Bioscience) were assessed by intracellular staining using solution AB (Thermo Fisher) and goat anti‐rabbit AF488 (Abcam). All samples were run using an LSR II (BD Biosciences) and analysed using FlowJo software (Treestar).
Magnetic beads were used to isolation of CD8⁺ and CD4⁺ T cells (Miltenyi Biotec) according to the manufacturer's instructions. The purity of T cell subsets was assessed by flow cytometry.

Callender L.A., Carroll E.C., Bober E.A., Akbar A.N., Solito E, & Henson S.M. (2019). Mitochondrial mass governs the extent of human T cell senescence. Aging Cell, 19(2), e13067.

+ Open protocol

+ Expand

Immunostaining of Vascular Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit anti-mouse GFP antibody, Rabbit anti-mouse CD3 antibody and goat anti-rabbit AF488 were purchased from abcam; rat anti-mouse CD31, CD144 and MHC-II molecule monoclonal antibody were purchased from eBioscience; goat anti-rat or anti-rabbit Cy3, anti-fluorescence quenching agent and rabbit anti-mouse CD31 polyclonal antibody were purchased from Servicebio; goat anti-rat AF488 was purchased from CST; EGM-2 medium was purchased from Lonza; Fibronectin was purchased from EMD Millipore; Anti-Mouse-CD31-V450, anti-Mouse-VEGFR2-PE and anti-Mouse-CD45-PerCP Cy7 were bought from BD; 7-AAD was purchased from Nanjing KGI Biotech Co., Ltd.; mouse endothelial cell growth factor (VEGF) was bought from Proteintech; Accutase-Enzyme Cell Detachment Medium was purchased from Thermo Fisher Company.

Wang W., Ye Y., Du Y., Xu Z., Yuan K., Wang Y., Adzraku S.Y., Li Y., Xu K., Qiao J., Ju W, & Zeng L. (2022). EPC infusion ameliorates acute graft-versus-host disease-related endothelial injury after allogeneic bone marrow transplantation. Frontiers in Immunology, 13, 1019657.

+ Open protocol

+ Expand

Multimodal Imaging of Oxidative Stress and Calcium Channels in Neurons

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

We prepared brain sections and performed parvalbumin (PV)/Wisteria floribunda agglutinin (WFA)/8-hydroxy-2′-deoxyguanosine (8-oxo-dG) triple immunofluorescence on males as described in [18 (link)] and Supplemental information. Primary antibodies were mouse monoclonal anti-8-oxo-dG (AMS Biotechnology, Switzerland), rabbit polyclonal anti-PV (Swant, Switzerland), biotin-conjugated lectin WFA (Sigma-Aldrich, Switzerland), while secondary antibodies were goat anti-mouse AF488 (Life Technologies, USA), goat anti-rabbit IgG CY3 (Chemicon International, USA) and streptavidin 405 conjugate (Millipore Corporation, USA).
We carried out CaV3.2/PV and CaV3.3/PV immunohistology as indicated in Supplemental information using the following primary [rabbit anti-CaV3.2 (Santa Cruz Biotechnology, USA); rabbit anti-CaV3.3 (Alomone labs, Israel), sheep anti-PV (R&D systems)] and secondary [goat anti-rabbit AF488 and donkey anti-sheep AF594 (Abcam, UK)] antibodies. Confocal image acquisition, and quantification are described in Supplemental information.

El Khoueiry C., Cabungcal J.H., Rovó Z., Fournier M., Do K.Q, & Steullet P. (2022). Developmental oxidative stress leads to T-type Ca2+ channel hypofunction in thalamic reticular nucleus of mouse models pertinent to schizophrenia. Molecular Psychiatry, 27(4), 2042-2051.

+ Open protocol

+ Expand

Immunofluorescence Assay for Kidney Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence on kidneys was performed as previously described [78 (link)]. Briefly, paraffin-embedded kidneys deparaffinized with xylene and rehydrated with graded ethanol washes. Antigen retrieval was performed using sodium citrate buffer in a pressure cooker. Endogenous peroxidases were quenched with 10% H₂O₂. Background Buster blocking solution (Innovex Biosciences, Richmond, CA) was applied to each sample prior to incubation in primary antibody. Slides were then incubated sequentially with rabbit polyclonal anti-SA (Abcam) and rat anti-mouse Ly-6G (clone 1A8) for 1 hour at room temperature or overnight at 4°C. Slides were then incubated sequentially with goat anti-rat AF-555 (Invitrogen) or goat anti-rabbit AF488 (Abcam) for 1 hour at room temperature. Slides were then mounted using Vectashield Antifade Reagent with DAPI (Vector Labs). Slides were imaged at 25°C, 20x magnification, using the Nikon A1 HD25 confocal microscope (Nikon Instruments Inc, Melville, NY). Images were analyzed with NIS Elements Viewer 4.20 software.

Visvabharathy L., Genardi S., Cao L., He Y., Alonzo F I.I.I., Berdyshev E, & Wang C.R. (2020). Group 1 CD1-restricted T cells contribute to control of systemic Staphylococcus aureus infection. PLoS Pathogens, 16(4), e1008443.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!