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Chromium potassium sulfate

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Chromium potassium sulfate is a chemical compound used in various laboratory applications. It is a crystalline solid that serves as a source of chromium ions. The compound's core function is to provide chromium in chemical reactions and analyses requiring this element.

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Lab products found in correlation

Round bottom polystyrene test tube, by BD (1 mentions) Gelatin, by Merck Group (1 mentions) Fp1012, by PerkinElmer (1 mentions) Sk 4100, by Vector Laboratories (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Gelatin, by Thermo Fisher Scientific (1 mentions) Cresyl violet, by Merck Group (1 mentions) Cresyl violet with acetate, by Merck Group (1 mentions)

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Potassium sulfate (43 citations)

3 protocols using chromium potassium sulfate

1

Neural Sphere Preparation and Cryosectioning

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Other spheres were dissociated and reseeded at low density, 2.5 × 104/mL, to form aggregate spheres or reaggregated by seeding at high density, 2.5 × 106/mL, to form reaggregated spheres. Spheres were selected based on size, < 200 μm, 200–500 μm or > 500 μm, placed in a 5mL round-bottom polystyrene test tube (Falcon) and similarly fixed, equilibrated in 30% sucrose, and embedded in Tissue-Tek. Gentle removal of each solution leaving the neurospheres to settle onto the bottom of the tube during each equilibration step ensured neurospheres were not damaged during processing. Neurospheres were cryosectioned at a thickness of 8 μm before being mounted onto slides coated with 0.5% w/v gelatin in 0.05% w/v chromium potassium sulfate (Sigma) for immunostaining.
Weible MW I.I., Lovelace M.D., Mundell H.D., Pang T.W, & Chan-Ling T. (2023). BMPRII+ neural precursor cells isolated and characterized from organotypic neurospheres: an in vitro model of human fetal spinal cord development. Neural Regeneration Research, 19(2), 447-457.
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2

PTEN Immunohistochemistry in AAV-Cre Tissue

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To address the PTEN deletion following AAV-Cre injection, free-flating coronal sections through the brain were incubated in 1% hydrogen peroxide for 15 min. After blocking in Tyramide Signal Amplification (TSA) blocking buffer (0.5g blocking reagent/ 40ml TBS, PerkinElmer, FP1012) sections were incubated in primary antibody at RT overnight (rabbit anti-PTEN, Cell Signaling 9188S, 1:250). Sections were washed in TBS and incubated with secondary antibody (donkey anti-rabbit HRP, Jackson Immunolabs 711-065-152, 1:250) in TSA blocking buffer for 2 hours at RT. Following a wash in TBS, sections were stained with 3–3′ diaminobenzidine (DAB, Vector Labs SK-4100) for 5 min, rinsed in TBS, mounted on gelatin subbed slides in weak mounting solution (0.5% gelatin and 0.05% chromium potassium sulfate, Sigma Aldrich, St. Louis, MO), air-dried and cover-slipped with DPX mounting medium.
Danilov C.A, & Steward O. (2015). Conditional genetic deletion of PTEN after a spinal cord injury enhances regenerative growth of CST axons and motor function recovery in mice. Experimental neurology, 266, 147-160.
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3

Tissue Preparation for Myelin and Nissl Staining

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In brief, every 10th section of the lesion site was mounted onto slides (Fisher Scientific, Pittsburgh, PA) coated with chrom-alum and poly-L-lysine layers (chromium potassium sulfate and poly-L-lysine, Sigma-Aldrich, St. Louis, MO; gelatin, Fisher Scientific). Tissue was fume fixed on the slides with 4% paraformaldehyde to enhance adherence. Tissue was first dipped in dH2O, and then dehydrated in increasing increments of alcohol (70%, 95%,100%). After immersing in xylene (10 minutes), tissue was rehydrated in decreasing increments of alcohol, then water and placed in an aqueous myelin dye (Eriochrome Cyanine R; Fluka, St. Louis, MO) for 10 minutes. After washing in water, differentiation took place in 1% ammonium hydroxide (1 minute). Tissue was then placed in 0.5% cresyl violet (cresyl violet with acetate, Sigma-Aldrich, St. Louis, MO) for 3 minutes, washed thoroughly in water, immersed in 70% alcohol, and then differentiated using < .2% glacial acetic acid in 95% alcohol. Following this, tissue was dehydrated again in increasing increments of alcohol, immersed in xylene and coverslipped with DPX (Fluka, St. Louis, MO).
Mondello S.E., Jefferson S.C., Tester N.J, & Howland D.R. (2015). Impact of treatment duration and lesion size on effectiveness of chondroitinase treatment post-SCI. Experimental neurology, 267, 64-77.
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