The largest database of trusted experimental protocols

V plex custom mouse cytokine assay

Manufactured by Mesoscale
Sourced in United States

The V-PLEX Custom Mouse Cytokine assay is a multiplex immunoassay designed to quantitatively measure multiple cytokines in mouse biological samples. The assay utilizes electrochemiluminescence detection technology to provide sensitive and reproducible measurements of analytes of interest.

Automatically generated - may contain errors

3 protocols using v plex custom mouse cytokine assay

1

Cytokine Levels Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum levels of IFN-γ, IL-10 and TNF-α were determined using the V-PLEX Custom Mouse Cytokine assay (Meso Scale Discovery, Rockville, USA) according to the manufacturer’s instructions.
+ Open protocol
+ Expand
2

Serum Cytokine and Antibody Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood was collected from CO2 euthanized mice via cardiac puncture, centrifuged (15 minutes, 10.000xg, 25°C), and serum was kept at -20°C. Serum levels of IFN-γ, IL-6, IL-10, IL-12p70, TNF and KC (CXCL1) were determined using the V-PLEX Custom Mouse Cytokine assay (Meso Scale Discovery, Maryland, USA). Serum MIF levels were measured by ELISA as recommended by the suppliers (R&D Systems). Alternatively, culture medium concentrations of MIF, TNF, CCL2 and KC (R&D Systems) as well as IFN-γ, IL-6 and IL-10 (Pharmingen) were determined by ELISA as recommended by the suppliers. Parasite-specific IgG responses were determined using soluble lysate freshly prepared from DEAE-purified T. congolense parasites recovered from WT mice at the peak of infection (around day 7). Lysate was coated overnight at 10 μg/ml PBS in 96-well Maxisorp plates (NUNC). Plates were washed (0.1% Tween 20 in PBS) and blocked (1% BSA in PBS) for 1 hour. Next, plates were washed and the sera (100 μl) serially diluted starting from 1/100 in blocking buffer were added. The ELISA was subsequently performed as described by the suppliers (SBA Clonotyping system-HRP kit (SouthernBiotech, USA)). As negative controls, blood samples incubated on lysate-free plates were used. The OD450nm recorded on lysate-free plates was subtracted from the OD450nm recorded on lysate-coated plates.
+ Open protocol
+ Expand
3

Quantifying Cytokine Levels in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tail vein blood samples were collected in heparinized capillary tubes and plasma was collected following centrifugation (15 minutes, 10.000 × g, 4 °C). Plasma levels of IFN-γ, IL-6, IL-10, IL-12p70, IL1β, TNF-α and CXCL1 were determined using the V-PLEX Custom Mouse Cytokine assay (Meso Scale Discovery, Maryland, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!