Hs895 t

We imaged and analyzed four isogenic cell lines, termed SW^{28 (link),29 (link)}, HS, TE, and WM. The SW cells are a pair of isogenic cancer cell lines: SW480 (ATCC CCL-228)—colorectal adenocarcinoma cells, and SW620 (ATCC CCL-227)—a metastatic form of these cancer cells collected from a lymph node of the same patient. The HS cells are a pair of a normal skin cell line, HS895.Sk (ATCC CRL-7636) and a melanoma cancer cell line, HS895.T (ATCC CRL-7637), taken from the same patient. The TE cells are a pair of a normal skin cell line, TE353.Sk (ATCC CRL-7761), and a melanoma cancer cell line, TE354.T (ATCC CRL-7762), are both taken from the same patient. The WM cells are a pair of a melanoma skin cell line, WM115 (ATCC CRL-1675), and a metastatic melanoma skin cell line, WM266.4 (ATCC CRL-1676), both taken from the same patient.
All the cells were grown in Dulbecco's modified Eagle medium (ATCC, SN. 30-2002), supplemented with 10% fetal bovine serum (BI, SN. 04-007-1A). 2 mM L-glutamine (BI, SN. 03-020-1B) were added for the WM cell lines. The cells were incubated under standard humidity and temperature of 37 °C with 5% CO₂ until 80% confluence was reached. Before imaging, the cells were trypsinized for suspension and supplemented with a suitable medium.

Ten human melanoma cell lines (A375, A375M, CHL-1, MEL224, MEL501, MEL505, MEWO, RPMI7951, SKMEL24, and SKMEL3) were kindly provided by Andreja Ambriović Ristov, PhD and Neda Slade, PhD. Cell lines HS895.SK (ATCC CRL-7636; Accession number CVCL_0992), HS895.T (ATCC CRL-7637; Accession number CVCL_0993), HS940.T (ATCC CRL-7691; Accession number CVCL_1038) and SKMEL2 (ATCC HTB-68; Accession number CVCL_0069) were purchased from the ATCC (Manassas, VA, USA). HS895.SK cell line represents a healthy control: skin keratinocytes isolated from the same patient as the HS895.T melanoma cell line. All cell lines were maintained in recommended medium: Dulbecco’s Modified Eagle Medium (Merck KgaA, Darmstadt, Germany), RPMI 1640 medium (Merck KgaA, Darmstadt, Germany), or Eagle’s Minimum Essential Medium (Merck KgaA, Darmstadt, Germany), supplemented with 10% FBS (Merck KGaA, Darmstadt, Germany), 1 mM sodium pyruvate, 1% streptomycin/penicillin and 4 mM L-glutamine (Gibco Thermo Fisher Scientific, Waltham, MA, USA).