The number of neutrophils, B cells, and CD4 + T cells was analyzed by flow cytometry. Briefly, peripheral blood mononuclear cells (PBMCs) from mice in different groups were stained with anti-mouse CD45 (BD Biosciences, 561,037, USA), anti-mouse CD4 (BD Biosciences, 553,651, USA), anti-mouse B220 (ebioscience, 69–0452-82, USA), anti-mouse CD11b (BD Biosciences, 550,993, USA), anti-mouse Ly6G(BD Biosciences, 560,599, USA), anti-mouse Ly6C (ebioscience, 25–5932-80, USA) for 30 min. After washing with PBS, the cells were analyzed with BD FACSCanto II flow cytometer.
Anti mouse ly6c
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-mouse Ly6C is a laboratory reagent used for the detection and analysis of the Ly6C cell surface antigen in mouse samples. It is a useful tool for the identification and characterization of specific cell populations in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse b220, by Thermo Fisher Scientific (2 mentions)
Anti mouse ly6g, by BD (1 mentions)
Anti mouse cd4, by BD (1 mentions)
Anti mouse cd45, by BD (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Anti mouse f4 80, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd11c, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd45, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd335 nkp46, by Thermo Fisher Scientific (1 mentions)
Anti mouse ly6g gr 1, by Thermo Fisher Scientific (1 mentions)
Lsrfortessa, by BD (1 mentions)
2 protocols using anti mouse ly6c
1
Quantifying Immune Cell Populations
2
Multiparametric Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TILs were incubated with the following panel of antibodies according to the manufacturer's instructions: anti-mouse CD45, anti-mouse B220, anti-mouse CD11b, anti-mouse CD11c, anti-mouse F4/80, anti-mouse Ly-6C, anti-mouse Ly-6G (Gr-1), and anti-mouse CD335 (NKp46) (eBioscience). Stained samples were assayed using LSRFortessa (BD). Results were analyzed using Flow-Jo software (BD). NK cells were identified by CD45 and CD335 (NKp46) coexpression. B cells displayed CD45 and B220 expression; dendritic cells were characterized by CD45 and CD11c; macrophages displayed CD45, CD11b, and F4/80 expression; monocytes displayed CD45, CD11b, and Ly-6C; and myeloid-derived suppressor cells expressed CD45, CD11b, and Ly-6G (Gr-1). All antibodies were used at a concentration of 4 µg/mL (1:50 dilution).
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