S adenosyl l methionine sam

Manufactured by Merck Group

Sourced in France, United States, China

S-adenosyl-L-methionine (SAM) is a naturally occurring compound found in all living cells. It serves as a methyl group donor in various biochemical reactions, playing a crucial role in cellular metabolism and other important biological processes.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (3 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Glucose 6 phosphate dehydrogenase g6pd, by Merck Group (1 mentions) Glucose 6 phosphate (g6p), by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) β nicotinamide adenine dinucleotide phosphate nadp, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Anaero pouch anaero, by Mitsubishi (1 mentions) Mas 5, by Chem Service (1 mentions)

Close spelling variants of this product

S-(5′-Adenosyl)-L-Methionine Iodide (SAM) S-(5′-Adenosyl)-l-methionine p-toluenesulfonate salt (SAM) S-adenosyl-methionine (SAM; S-adenosyl-l-methionine L-methionine Methionine

5 protocols using s adenosyl l methionine sam

Biocatalytic Production of Monoterpenoid Indole Alkaloids

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Vincadifformine was prepared by the hydrogenation of tabersonine as described in [68 (link)]. Lochnericine, 19-hydroxytabersonine and 16-hydroxytabersonine were produced by the 6,7-epoxidation of tabersonine, the 19-hydroxylation or 16-hydroxylation of tabersonine by yeast expressing TEX1, T19H or T16H2 from C. roseus, respectively [23 (link),53 (link)]. S-adenosyl-L-methionine (SAM) was purchased from Sigma-Aldrich (St-Quentin-Fallavier, France).

Stander E.A., Sepúlveda L.J., Dugé de Bernonville T., Carqueijeiro I., Koudounas K., Lemos Cruz P., Besseau S., Lanoue A., Papon N., Giglioli-Guivarc’h N., Dirks R., O’Connor S.E., Atehortùa L., Oudin A, & Courdavault V. (2020). Identifying Genes Involved in Alkaloid Biosynthesis in Vinca minor through Transcriptomics and Gene Co-Expression Analysis. Biomolecules, 10(12), 1595.

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Crystallization of G9a/GLP-Compound 1 Complex

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G9a or GLP in complex with compound 1 was crystallized according to a previously published protocol.^{43 (link)} Briefly, purified GLP and G9a were mixed with S-adenosyl-L-methionine (SAM; Sigma-Aldrich, USA) and compound 1 (protein/SAM/compound 1 molar ratio of 1:2:4). The mixture was coconcentrated to about 20 mg/mL and crystallized with the use of the sitting drop method at 17 °C. The GLP–SAM–compound 1 and G9a–SAM–compound 1 complexes were crystallized in 2% v/v 1,4-dioxane and 10% w/v polyethylene glycol 20 000 for G9a and 0.2 magnesium formate and 15% w/v polyethylene glycol 3350 for GLP. X-ray diffraction data were collected at 100 K at the NE-CAT beamline 24-ID-E of the Advanced Photon Source at Argonne National Laboratory. The data were processed with MOSFLM, SCALA, and other programs from the CCP4 suite. The structures of the G9a and GLP complexes were solved by molecular replacement using the PHASER. Phenix was used for structure refinement. The graphic program COOT was used for model building and visualization.

Park K.S., Xiong Y., Yim H., Velez J., Babault N., Kumar P., Liu J, & Jin J. (2022). Discovery of the First-in-Class G9a/GLP Covalent Inhibitors. Journal of medicinal chemistry, 65(15), 10506-10522.

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Quantification of Antioxidant Compounds

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The authentic standards of ellagic acid (EA), pyrogallol, and gallic acid (GA) were purchased from the Chengdu MUST Bio-Technology CO. Ltd. (Chengdu, China), phosphate-buffered saline (PBS, pH = 7.4, 0.01 M), HPD resin was purchased from Tianjin Baowen Chemical Technology CO. Ltd. (Tianjin, China), anaerobic bags (AnaeroPouch Anaero, Mitsubishi Gas Chemical Company Inc., Tokyo, Japan), GAM broth medium was bought from Qingdao Haibo Biology CO. Ltd. (Qingdao, China), β-nicotinamide adenine dinucleotide phosphate (NADP), glucose-6-phosphate (G-6-P), glucose-6-phosphate dehydrogenase (G-6-PD), dithiothreitol, and S-adenosyl-L-methionine (SAM) were obtained from Sigma Chemical CO. (St. Louis, MO, USA). Acetonitrile, methanol, and formic acid were of HPLC-grade, which were purchased from Thermo Fisher Scientific CO. Ltd. (Waltham, MA, USA). Purified water was prepared by using Milli-Q System (Millipore, Billerica, MA, USA). All other analytical-grade reagents were provided by the Sinopharm Chemical Reagent CO. Ltd. (Shanghai, China).

Tu J., Li Q, & Zhou B. (2021). The Tannins from Sanguisorba officinalis L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS2 Analysis. Molecules, 26(13), 4053.

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Enzymatic Assays for Protein Glycosylation and Histone Methylation

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1 μg of recombinant expressed His‐SECΔN (aa 592–952, containing the OGT domain of SEC) was incubated with 1 μg His‐ATX1ΔN (aa 801–1,062, containing the SET domain) and 50 μM UDP‐N‐acetylglucosamine in 25 μl of reaction system for 1 h at 37°C. Immunoblotting analysis was conducted to detect O‐GlcNAc modification of proteins with antibody CTD110.6.
For ATX1 histone methyltransferase enzyme activity assay, purified His‐ATX1ΔN was incubated with recombinant expressed H3 (Millipore) and unlabeled S‐adenosyl‐L‐methionine (SAM, Sigma) in methyltransferase buffer. After the HMTase assay, the products were analyzed by immunoblot analysis with the indicated antibodies.

Xing L., Liu Y., Xu S., Xiao J., Wang B., Deng H., Lu Z., Xu Y, & Chong K. (2018). Arabidopsis O‐GlcNAc transferase SEC activates histone methyltransferase ATX1 to regulate flowering. The EMBO Journal, 37(19), e98115.

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Synthesis and Characterization of MAs

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Arsenicals and S-adenosyl-L-methionine (SAM) were purchased from Sigma-Aldrich. MAs(V) was obtained from Chem Service, Inc., West Chester, PA. MAs(III) was prepared as described (Reay and Asher, 1977 (link)). All reagents were analytical grade.

Zhang J., Chen J., Wu Y.F., Liu X., Packianathan C., Nadar V.S., Rosen B.P, & Zhao F.J. (2022). Functional characterization of the methylarsenite-inducible arsRM operon from Noviherbaspirillum denitrificans HC18. Environmental microbiology, 24(2), 772-783.

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