The human GC cell lines MKN1, MKN7, MKN28, MKN45, MKN74, SH-10-TC, NUGC-3, NUGC-4, AGS, GSU, and KE-39 were purchased from RIKEN BioResource Center (Tsukuba, Japan). Cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% fetal bovine serum with 100 U/mL penicillin and 100 U/mL streptomycin sulfate and cultured in a humidified 5% CO2 incubator at 37°C.
Nugc 3
Manufactured by RIKEN BioResource Center
Sourced in Japan
The NUGC-3 is a cell line derived from a human gastric adenocarcinoma. It is a commonly used in vitro model for cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Mkn45, by RIKEN BioResource Center (3 mentions)
Mkn74, by RIKEN BioResource Center (3 mentions)
Mkn28, by RIKEN BioResource Center (2 mentions)
Nugc 4, by RIKEN BioResource Center (2 mentions)
Katoiii, by RIKEN BioResource Center (2 mentions)
Sh 10 tc, by RIKEN BioResource Center (1 mentions)
Ke 39, by RIKEN BioResource Center (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Tsgh 9201, by RIKEN BioResource Center (1 mentions)
Penicillin streptomycin, by Sartorius (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
4 protocols using nugc 3
1
Culturing Human Gastric Cancer Cell Lines
2
Culturing Human Gastric Cancer Cells
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Human gastric cancer cell lines (MKN7, MKN45, MKN74, KATOIII, and NUGC3) were purchased from the Riken Bioresource Center (Cell Bank, Ibaraki, Japan) and the Japanese Collection of Research Bioresources (JCRB Cell Bank, Osaka, Japan). All cell lines were cultured in culture medium (RPMI 1640 medium [Sigma-Aldrich, St. Louis, MO] containing 10% heat-inactivated fetal bovine serum [FBS, Life Technologies, Carlsbad, CA], 100 U/mL of penicillin, 100 μg/mL of streptomycin, and 0.25 μg/mL of amphotericin B [Antibiotic-Antimycotic; Life Technologies]) at 37°C in 5% CO2 with 95% humidity.
3
Authentication and Culturing of Gastric Cell Lines
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Vero (African green monkey kidney normal cell line), MKN1, MKN28, MKN45, MKN74, NUGC3, NUGC4, KATOIII, and N87 (human gastric cancer cell lines) cells were obtained from the RIKEN BioResource Center. All cell lines were authenticated according to the Cell Line Verification Test Recommendations of ATCC Technical Bulletin no. 8 (2008). TMK-1 cells, a human gastric cancer cell line, were provided by Dr Eiichi Tahara (Hiroshima University, Hiroshima, Japan). All human gastric cancer cell lines were cultured in RPMI-1640 and Vero cells were cultured in Dulbecco's modified Eagle's medium both supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc.).
4
Culturing Human Gastric Cancer Cells
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Human gastric cancer cells AGS, NUGC-3, and TSGH-9201 were purchased from the Bioresource Collection and Research Center (Food Industry Research and Development Institute, Hsinchu, Taiwan) and cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% antibiotic (penicillin-streptomycin, Biological Industries, Kibbutz Beit HaEmek, Israel), and incubated at 37 °C in a 5% CO2-containing incubator. When the cells were about 70−80% grown, sub-cultures were grown with PBS added to 1× Trypsin-EDTA (TE, Biological Industries, Kibbutz Beit HaEmek, Israel). The optimal passage ratios of these gastric cancer cells were 1:4–1:8.
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