Anti polii

Manufactured by Abcam

Anti-PolII is an antibody that recognizes the RNA polymerase II (Pol II) enzyme, which is responsible for transcribing DNA into messenger RNA in eukaryotic cells. This antibody can be used in various applications, such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP), to study the distribution and regulation of Pol II in biological samples.

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Lab products found in correlation

Anti h3k36me3, by Abcam (2 mentions) Anti flag, by Merck Group (1 mentions) Proteinase k, by Promega (1 mentions) Rnase a, by Thermo Fisher Scientific (1 mentions) Anti h3k9me2, by Abcam (1 mentions) Anti h3k18ac, by Abcam (1 mentions) Anti h3k23ac, by Merck Group (1 mentions) Anti myc, by Merck Group (1 mentions) Dynabead, by Thermo Fisher Scientific (1 mentions) Anti creb, by Merck Group (1 mentions) Anti h3k4me3, by Merck Group (1 mentions) Anti atf4, by Santa Cruz Biotechnology (1 mentions) Anti cebp, by Santa Cruz Biotechnology (1 mentions) Gfp 1010, by Aves Labs (1 mentions)

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PolII (4 citations)

3 protocols using anti polii

Chromatin Immunoprecipitation and Microscopy Experiments

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The following commercially available antibodies were used for chromatin immunoprecipitation experiments: Anti-H3K4me3 (Millipore, catalog number 04–745; RRID:AB_1163444), Anti-H3K36me3 (Abcam, catalog number ab9050; RRID:AB_306966), Anti-CREB (Millipore, catalog number 06–519; RRID:AB_310153), Anti-PolII (Ser5-phosphorylated) (Abcam, catalog number ab5131; RRID:AB_449369), Anti-PolII (Ser2-phosphorylated) (Abcam, catalog number ab5095; RRID:AB_304749), Anti-CBP/p300 (Millipore, catalog number 05–257; RRID:AB_11213111), Anti-C/EBPß (Santa Cruz, catalog number sc-150; AB_2260363) or Anti-ATF4 (Santa Cruz, catalog number sc-200; RRID:AB_2058752). The following commercially available antibodies were used for microscopy experiments: anti-RFP (Invitrogen R10367; RRID:AB_2315269), anti-GFP (Aves Labs GFP1010; RRID:AB_2307313), goat anti-rabbit Alexa 555 (Invitrogen A21428; RRID: AB_10561552) and goat anti-rabbit Alexa 488 (Invitrogen A11039; RRID:AB_142924). The following cell lines were used: rat H19-7 (ATCC CRL-2526; RRID: CVCL_H781), mouse Neuro-2a (ATCC CCL-131; RRID:CVCL_0470), human HEK-293FT (Invitrogen R70007; RRID:CVCL_6911), human HEK-293T (ATCC CRL-3216; RRID:CVCL_0063).

Mathew R.S., Tatarakis A., Rudenko A., Johnson-Venkatesh E.M., Yang Y.J., Murphy E.A., Todd T.P., Schepers S.T., Siuti N., Martorell A.J., Falls W.A., Hammack S.E., Walsh C.A., Tsai L.H., Umemori H., Bouton M.E, & Moazed D. (2016). A microRNA negative feedback loop downregulates vesicle transport and inhibits fear memory. eLife, 5, e22467.

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Chromatin Immunoprecipitation (ChIP) Protocol

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Chromatin immunoprecipitation (ChIP) was performed as previously reported[29 (link)]. Briefly, cortex tissue was dissected from 6-8-week-old mice, minced and crosslinked in 1% formaldehyde (wt/vol) and sonicated using a Misonix 3000. Antibody was first bound to Dynabeads and then incubated with sheared chromatin overnight at 4°C. After 4 washes with RIPA buffer and 1 wash with TE buffer, bound chromatin was eluted and reverse crosslinked at 65°C overnight. Eluted DNA was treated with RNase A (Thermo Scientific) and proteinase K (Promega), purified by phenol-chloroform extraction and dissolved in water. Antibodies used were: anti-Flag (Sigma M2), anti-LEDGF (Bethyl A300-847A), anti-H3K36me3 (Abcam ab9050), and anti-PolII (Abcam ab5408). Primer sequence for ChIP-qPCR is provided in S8 Table.

Li R., Dong Q., Yuan X., Zeng X., Gao Y., Chiao C., Li H., Zhao X., Keles S., Wang Z, & Chang Q. (2016). Misregulation of Alternative Splicing in a Mouse Model of Rett Syndrome. PLoS Genetics, 12(6), e1006129.

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ChIP-seq Protocol for Histone Modifications

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The ChIP assay was performed as described (Wierzbicki et al., 2008 (link)). Dynabeads (Invitrogen, cat# 10003D) were used for pre-clearing and antibody binding. The antibodies were anti-H3K9me2 (Abcam, cat# ab1220), anti-H3K18ac (Abcam, cat# ab1191), anti-H3K23ac (Millipore, cat#07-355), anti-MYC (Millipore, cat#05-724), and anti-Pol II (Abcam, cat#ab5408).

Lang Z., Lei M., Wang X., Tang K., Miki D., Zhang H., Mangrauthia S.K., Liu W., Nie W., Ma G., Yan J., Duan C.G., Hsu C.C., Wang C., Tao W.A., Gong Z, & Zhu J.K. (2015). The methyl-CpG-binding protein MBD7 facilitates active DNA demethylation to limit DNA hyper-methylation and transcriptional gene silencing. Molecular cell, 57(6), 971-983.

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