N arachidonoyl glycine

Manufactured by Cayman Chemical

N-arachidonoyl glycine is a naturally occurring lipid found in the central nervous system and other tissues. It functions as an endogenous ligand for certain G protein-coupled receptors.

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Lab products found in correlation

Mouse pm20d1 flag, by Addgene (2 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Arachidonic acid, by Merck Group (1 mentions) Anandamide, by Merck Group (1 mentions) Pf 3845, by Selleck Chemicals (1 mentions) Wwl70, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions) Anti apoa1, by Abcam (1 mentions) Anti apob, by Abcam (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) Neurobasal medium, by Thermo Fisher Scientific (1 mentions) Bupropion hydrochloride, by Merck Group (1 mentions) Mouse anti calnexin, by BD (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti transferrin receptor, by Thermo Fisher Scientific (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Spc 108, by BD (1 mentions) Anti ubiquitin, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Deuterium-labeled N-arachidonoyl glycine (d8NAGly; (2 citations)

3 protocols using n arachidonoyl glycine

Synthesis and Characterization of N-Acyl Amino Acids

Cited 2 times

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N-arachidonoyl glycine, N-arachidonoyl serine, and N-arachidonoyl-taurine were purchased from Cayman. N-arachidonoyl phenylalanine was purchased from Abcam. Arachidonic acid, anandamide, and WWL70 were purchased from Sigma-Aldrich. PF-3845 was purchased from Selleckchem. MAFP and EDTA were purchased from Fisher. Talabostat was purchased from R and D. Non-commercially available N-acyl amino acids were synthesized as previously described (Lin et al., 2018 (link); Long et al., 2016 (link)). Plasmids were obtained from the following sources: mouse PM20D1-flag (Addgene 132682), mouse FAAH-flag (Origene MR209084), mouse ACY1-flag (Origene MR206415), mouse CNDP1-flag (Origene MR219018), mouse CNDP2-flag (Origene MR207616), and mouse PM20D2-flag (MR222068).

Kim J.T., Terrell S.M., Li V.L., Wei W., Fischer C.R, & Long J.Z. (2020). Cooperative enzymatic control of N-acyl amino acids by PM20D1 and FAAH. eLife, 9, e55211.

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N-arachidonoyl glycine photocrosslinking probe

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N-arachidonoyl glycine was purchased from Cayman. N-acyl amino acid photocrosslinking probe was synthesized as previously described (Long et al., 2016 ). Phenylalanine (BP391100) and oleic acid (AC270290050) were purchased from Fisher. BSA (protease free, fatty acid free, essentially globulin free) was purchased from Sigma (A7030). Pooled human plasma (IPLA-N) was purchased from Innovative Research. The following antibodies were used: anti-Flag M2 (Sigma F3165), anti-APOB (Abcam ab20737), anti-APOA1 (ab7614). The following plasmids were used: mouse AAV-PM20D1-flag (Addgene 132682), mouse PM20D1-flag (Addgene 84566). Adeno-associated viruses were produced at the Penn Vector Core. AAV-GFP virus was purchased from Penn Vector Core (AAV8.CB7.CI.eGFP.WPRE.rBG).

Kim J.T., Jedrychowski M.P., Wei W., Fernandez D., Fischer C.R., Banik S.M., Spiegelman B.M, & Long J.Z. (2020). A plasma protein network regulates PM20D1 and N-acyl amino acid bioactivity. Cell chemical biology, 27(9), 1130-1139.e4.

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GlyT2 Defective Phenotypes Rescue Screening

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Female Wistar rats were bred under standard conditions at the Centro de Biología Molecular Severo Ochoa (CBMSO) in accordance with procedures approved in the Directive 2010/63/EU of the European Union with approval of the Research Ethics Committee of the Universidad Autónoma de Madrid (Comité de Ética de la Investigación UAM, CEI-UAM). Rabbit and rat antibodies against N-terminus of GlyT2 were generated in house (Zafra et al. 1995) . Other primary antibodies used were: anti-transferrin receptor (Invitrogen, #13-6800), anti-E cadherin (a gift from Amparo Cano, UAM), rabbit anti-calnexin (StressMarq Biosciences, SPC-108), mouse anti-calnexin (BD Transduction Laboratories, clone 37), anti-ubiquitin (Santa Cruz, sc-8017, clone P4D1), anti-α-tubulin (Sigma-Aldrich, clone T-6074), anti-Myc (Cell Signaling, #2276) and anti-transferrin receptor (Invitrogen). For the screening of compounds with the potential to rescue GlyT2 defective phenotypes the following chemicals were used: bupropion hydrochloride (Sigma Aldrich), ibogaine hydrochloride (LGC Standards), ALX1393 (Santa Cruz Biotechnology), N-arachidonoyl glycine (Cayman chemicals) and 4-phenylbutirate (Sigma Aldrich). All other chemicals used were from Sigma Aldrich unless otherwise noticed. Neurobasal medium and B27 supplement were purchased from Invitrogen.

Rocha‐Muñoz A.d.l., Melgarejo E., Aragón C., & López‐Corcuera B. (2021). Rescue of two trafficking-defective variants of the neuronal glycine transporter GlyT2 associated to hyperekplexia.

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