The OPFR mixture was a combination of tris(1,3-dichloro-2-propyl)phosphate (TDCPP, Sigma Aldrich, St. Louis, MO, CAS#:13674-87-8, 95.6% purity), triphenyl phosphate (TPP, Sigma Aldrich, St. Louis, MO; CAS#:115-86-6, 99% purity), and tricresyl phosphate (TCP, AccuStandard, New Haven, CT, CAS#: 1330-78-5, 99% purity) at 1 mg/kg each. This dose was previously found to be effective in an adult exposure model in mice (Krumm et al 2018 (link)), in our recent maternal exposure study on neonatal and juvenile gene expression (Adams et al 2020 (link)), and in a developmental exposure model in rats focusing on exploratory behavior and locomotion (Baldwin et al 2017 (link); Patisaul et al 2013 (link)). To prepare the OPFR stock, each compound was then dissolved into 1 ml of acetone to create a for a stock solution. 100 μl of stock solution was dissolved into 10ml of sesame oil (Sigma-Aldrich) and allowed to vent for 24–48 h, as previously reported (Krumm et al 2018 (link)).
Tricresyl phosphate
Manufactured by AccuStandard
Sourced in United States
Tricresyl phosphate is a colorless, viscous liquid chemical compound. It is a type of organophosphate compound and serves as a plasticizer, flame retardant, and viscosity modifier in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Sesame oil, by Merck Group (1 mentions)
Triphenyl phosphate, by Merck Group (1 mentions)
Tdcpp, by Merck Group (1 mentions)
Tcep d12, by LGC (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Acetone, by Avantor (1 mentions)
N hexane, by Avantor (1 mentions)
Tnbp d27, by LGC (1 mentions)
Tphp d15, by LGC (1 mentions)
Tep d15, by LGC (1 mentions)
Triphenyl phosphate, by AccuStandard (1 mentions)
2 protocols using tricresyl phosphate
1
OPFR Mixture Preparation Protocol
2
Analytical Standards for Organophosphate Esters and Phthalates
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Eleven OPE standards including alkyl-OPEs (trimethyl phosphate (TMP), triethyl phosphate (TEP), tripropyl phosphate (TPrP), tributyl phosphate (TNBP) and tris (2-butoxy ethyl) phosphate (TBEP)), Cl-OPEs (tris(2-chloroethyl) phosphate (TCEP), tris(2-chloroisopropyl) phosphate (TCIPP) and tris(1,3-dichloroisopropyl) phosphate (TDCIPP)) and aryl-OPEs (triphenyl phosphate (TPhP), tricresyl phosphate (TCP) and 2-ethylhexyl diphenyl phosphate (EHDPP)) were purchased from Accustandard (USA). The internal standards for OPEs including TMP-d9, TEP-d15, TNBP-d27, TCEP-d12, TCIPP-d18, and TPhP-d15 were purchased from Toronto Research Chemicals (Canada). Six individual PAE analogues including diethyl phthalate (DEP), dimethyl phthalate (DMP), butyl benzyl phthalate (BBP), dibutyl phthalate (DnBP), diisobutyl phthalate (DiBP), and di-(2-ethylhexyl) phthalate (DEHP), were obtained from o2si smart solutions (USA). Internal standards for PAEs (DEP-d4 and DnBP-d4) were purchased from Dr. Ehrenstorfer GmbH (Germany). Acetone and n-hexane were HPLC-grade and obtained from J. T. Baker (USA). Methanol and acetonitrile applied in the instrumental analysis were obtained from Merck (Germany). The syringe filter with PTFE (Polytetrafluoroethylene) was provided by JinTeng (China).
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