The TANs were determined pathologically by H&E staining and immunohistochemistry (IHC). A rabbit polyclonal anti-myeloperoxidase (MPO) antibody (Cell Signaling Technology, MA, USA) was used, and H&E staining or IHC was performed on paraffin-embedded formalin-fixed tissues according to standard protocols. Neutrophil was defined as lobulated nuclei, and the cytoplasm was rich in reddish granules. Neutrophils engaged within the tumor tissue (including the neoplastic parenchyma and the tumor stroma) were referred to as TANs (17 (link)). All the slides containing tumor tissue were observed at low power, and the section with the most apparent neutrophilic aggregates within the tumor tissue was selected. Ten non-overlapping high-power fields (HPFs, 400-fold magnification, field diameter 0.55 μm) were observed continuously. However, the areas closely adjacent to mucosal erosion, ulcer, or infarct-like necrosis of tumor were excluded for neutrophil counting. Patients were divided into high- and low-TAN infiltration groups, based on a median TAN number of 10 per HPF in primary tumors.
Mpo antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The MPO) antibody is a laboratory tool used to detect and quantify the presence of the myeloperoxidase (MPO) protein in biological samples. MPO is an enzyme found in certain immune cells and plays a role in the body's defense against infections. The MPO) antibody can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to measure MPO levels in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mpo antibody
1
Quantifying Tumor-Associated Neutrophils
2
Evaluation of Neuroinflammation Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ipsilateral hemisphere was collected 24 hours after reperfusion and processed for a Western blot analysis as described previously. 14 The first antibodies were an anti-myeloperoxidase (MPO) antibody (Cell Signaling Technology, Danvers, MA, USA, dilution 1:2000) as a marker for neutrophils, an anti-matrix metalloproteinase (MMP)-9 antibody (Cell Signaling Technology, Danvers, MA, USA, dilution 1:2000), and anti-intercellular molecule-1 (ICAM-1) antibody (Abcam, Cambridge, MA, USA, dilution 1:2000). Western blot bands were quantified by densitometry using an image analysis system (ImageJ 1.37v; NIH Bethesda, MD, USA)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!