Erythrocytes cells were depleted from total mouse splenocytes using red blood cell lysis buffer (Sigma, cat. R7757). Mouse lymphocytes were cultured in RPMI 1640 (cat. 11875; Gibco) supplemented with 10% (v/v) inactivated FCS (Gibco), 0.0002% β-mercaptoethanol final (Sigma, cat. M7522), penicillin 50 units per liter - streptomycin 50 mg per liter (Sigma, cat. P4333), 1 mM sodium pyruvate final (Gibco, cat. 11360) non-essential amino acids (life technologies, cat. 11140) and 2 mM glutamine (US Biological, cat. G7120). HEK 293T cells were cultured in Dulbecco's Modified Eagle's Medium supplemented with 10% (v/v) inactivated FBS (Gibco). Saccharomyces cerevisiae strain W303 was cultured in YPD medium. Toxoplasma gondii (RH strain) tachyzoites were grown in human foreskin fibroblasts (HFF) cultured in Dulbecco's Modified Eagles Medium (DMEM; Invitrogen) supplemented with 10% tetracycline-free FBS (HyClone), 2 mM glutamine, 10 mM HEPES (pH 7.5), and 20 μg/ml gentamicin.
Sodium pyruvate final
Manufactured by Thermo Fisher Scientific
Sodium pyruvate final is a high-purity biochemical compound used in cell culture and other laboratory applications. It serves as a source of pyruvate, a key metabolite in cellular energy production pathways.
Automatically generated - may contain errors
Lab products found in correlation
Red blood cell lysis buffer, by Merck Group (2 mentions)
β mercaptoethanol final, by Merck Group (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Merck Group (2 mentions)
Inactivated, by Thermo Fisher Scientific (1 mentions)
Glutamine, by US Biological (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Tetracycline free fbs, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
2 protocols using sodium pyruvate final
1
Culturing Diverse Cell Types for Research
2
Cell Culture Protocols for Various Cell Types
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Erythrocytes cells were depleted from
total mouse splenocytes using red blood cell lysis buffer (Sigma,
cat. R7757). Mouse lymphocytes were cultured in RPMI 1640 (cat. 11875;
Gibco) supplemented with 10% (v/v) inactivated FCS (Gibco), 0.0002%
β-mercaptoethanol final (Sigma, cat. M7522), penicillin 50 units
per liter–streptomycin 50 mg per liter (Sigma, cat. P4333),
1 mM sodium pyruvate final (Gibco, cat. 11360) nonessential amino
acids (Life Technologies, cat. 11140) and 2 mM glutamine (US Biological,
cat. G7120). HEK 293T cells were cultured in Dulbecco’s Modified
Eagle’s Medium supplemented with 10% (v/v) inactivated FBS
(Gibco). Saccharomyces cerevisiae strain W303 was
cultured in YPD medium. Toxoplasma gondii (RH strain)
tachyzoites were grown in human foreskin fibroblasts (HFF) cultured
in Dulbecco’s Modified Eagles Medium (DMEM; Invitrogen) supplemented
with 10% tetracycline-free FBS (HyClone), 2 mM glutamine, 10 mM HEPES
(pH 7.5), and 20 μg/mL gentamicin.
total mouse splenocytes using red blood cell lysis buffer (Sigma,
cat. R7757). Mouse lymphocytes were cultured in RPMI 1640 (cat. 11875;
Gibco) supplemented with 10% (v/v) inactivated FCS (Gibco), 0.0002%
β-mercaptoethanol final (Sigma, cat. M7522), penicillin 50 units
per liter–streptomycin 50 mg per liter (Sigma, cat. P4333),
1 mM sodium pyruvate final (Gibco, cat. 11360) nonessential amino
acids (Life Technologies, cat. 11140) and 2 mM glutamine (US Biological,
cat. G7120). HEK 293T cells were cultured in Dulbecco’s Modified
Eagle’s Medium supplemented with 10% (v/v) inactivated FBS
(Gibco). Saccharomyces cerevisiae strain W303 was
cultured in YPD medium. Toxoplasma gondii (RH strain)
tachyzoites were grown in human foreskin fibroblasts (HFF) cultured
in Dulbecco’s Modified Eagles Medium (DMEM; Invitrogen) supplemented
with 10% tetracycline-free FBS (HyClone), 2 mM glutamine, 10 mM HEPES
(pH 7.5), and 20 μg/mL gentamicin.
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