FACS-sorted MIC2-GLuc reporter tachyzoites and bradyzoites, or bradyzoites purified by magnetic beads, were suspended with EC buffer and incubated with different agonists at 37°C for 10 min. ESA was collected by centrifugation and mixed with Pierce Gaussia Luciferase Glow Assay Kit reagent (Thermo Scientific), and luminescence was detected using a Cytation 3 Cell Imaging Multimode Imager (BioTek Instruments, Inc). Buffer control values were subtracted from their corresponding sample values to correct for background.
Pierce gaussia luciferase glow assay kit reagent
Manufactured by Thermo Fisher Scientific
The Pierce Gaussia Luciferase Glow Assay Kit is a reagent used to detect and quantify the activity of the Gaussia luciferase reporter enzyme. The kit provides a substrate solution that, when combined with a sample containing Gaussia luciferase, produces a bioluminescent signal that can be measured using a luminometer or other light detection instrument.
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Lab products found in correlation
2 protocols using pierce gaussia luciferase glow assay kit reagent
1
Quantifying Tachyzoite and Bradyzoite Secretion
2
Measuring Tachyzoite and Bradyzoite Egress
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FACS sorted MIC2-GLuc reporter tachyzoites and bradyzoites were suspended with EC buffer and incubated with different agonists at 37°C for 10 min. ESA was collected by centrifugation and mixed with Pierce™ Gaussia Luciferase Glow Assay Kit reagent (Thermo Scientific™) and luminescence was detected using a Cytation 3 Cell Imaging Multimode Imager (BioTek Instruments, Inc.). Buffer control values were subtracted from their corresponding sample values to correct for background.
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