Ti2 a inverted microscope

Manufactured by Nikon

The Ti2-A inverted microscope is a versatile laboratory instrument designed for a range of microscopy applications. It features a stable and ergonomic design, supporting high-quality imaging and accurate sample observation. The Ti2-A provides essential functionality for researchers and scientists working in various fields that require advanced microscopy capabilities.

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Lab products found in correlation

Ixon ultra 897, by Oxford Instruments (1 mentions) Fesh0450, by Thorlabs (1 mentions) Ldh d c 375, by PicoQuant (1 mentions) Felh0500, by Thorlabs (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Ds qi1mc camera, by Nikon (1 mentions)

4 protocols using ti2 a inverted microscope

Fluorescence Microscopy Experimental Setup

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Fluorescence
microscopy experiments were performed using a Nikon Ti2-A inverted
microscope. The samples are placed on the microscope’s table,
which was applied with a custom-made box to allow measurements under
inert conditions. The excitation light was from a 373 nm collimated
free-beam laser diode (LDH-D-C-375, PicoQuant), passing a clean-up
filter (370/36 BrightLine HC, Semrock) and a lambda fourth plate (355
nm, Edmund Optics). The beam was expanded using a 10× UV beam
expander (BE10-UVB, Thorlabs, Inc.) and then focused on the back-focal
plane of the objective to enable far-field microscopy. It entered
the microscope through the backside port and was mirrored to the sample
stage via a dichroic mirror (zt 375 RDC, Chroma). Emitted light from
the sample was collected by the objective and passed the dichroic
mirror to be led to a side port of the microscope. Here, it was spectrally
separated into two parts using color filters (FESH0450 and FELH0500,
Thorlabs) and a dichroic mirror (zt 514 RDC, Chroma) mounted on an
Optosplit II (Acal BFi Germany GmbH). The two resulting images represented
the wavelength regimes. The image detection was done using a back-illuminated
CCD camera (iXon Ultra 897, Andor). Time-resolved measurements were
realized by taking a series of images and subsequent post-procession
of the data with a self-written evaluation script.

Thomas H., Fries F., Gmelch M., Bärschneider T., Kroll M., Vavaleskou T, & Reineke S. (2021). Purely Organic Microparticles Showing Ultralong Room Temperature Phosphorescence. ACS Omega, 6(20), 13087-13093.

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Confocal Microscopy Imaging Protocol

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Image acquisition and reconstruction were carried out according to the description published by Velásquez et al. [18 (link)]. A ReScan confocal microscope (RCM 1.1 Visible, Confocal.nl) equipped with a fixed 50 µm pinhole size and combined with a Nikon Ti2-A inverted microscope was used to acquire fluorescence and confocal images. The RCM unit was connected to a Toptica CLE laser with the following excitations: 405/488/561/640 nm. Images were acquired using a scientific CMOS [complementary metal–oxide–semiconductor] (sCMOS) camera (pco.edge, PCO) with a CFI Plan Apochromat 60× lambda-immersion oil objective (NA 1.4/0.13; Nikon). The system was operated using NIS-Elements software (version 5.11). Images were acquired via z-stack optical series with a step size of 0.1 micron depth to cover all structures of interest within the analysed host cells. Z-series were displayed as maximum z-projections. Identical brightness and contrast conditions were applied for each data set within one experiment using Fiji software [19 (link)].

Rojas-Baron L., Senk K., Hermosilla C., Taubert A, & Velásquez Z.D. (2024). Toxoplasma gondii modulates the host cell cycle, chromosome segregation, and cytokinesis irrespective of cell type or species origin. Parasites & Vectors, 17, 180.

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Fluorescent Staining of Fungus

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The fluorescent stains used in this study included CFW, PI, and CFDA and all staining was performed as previously described (7 (link)). CFW and PI were used at 12.5 µg/mL final concentration and CFDA was employed at 50 µg/mL. All staining solutions were prepared in PBS. For CFW and PI staining, coverslips with adherent fungus were washed twice with phosphate buffered saline (PBS) pH 7.4 (Gibco) for 5 min, then submerged in staining solution for 5 min. Following this incubation, coverslips were washed twice additionally in PBS for 10 min before being mounted onto glass microscope slides. CFW, PI, and CFDA fluorescence was visualized using 4’,6-diamidino-2-phenylindole (DAPI), tetramethylrhodamine (TRITC), and GFP filters, respectively. Fluorescence microscopy was conducted with a Nikon TI2-A inverted microscope equipped with a Prime BSI express monochrome camera or a Nikon NiU microscope equipped with a Nikon DS-Qi1Mc camera.

Thorn H.I., Guruceaga X., Martin-Vicente A., Nywening A.V., Xie J., Ge W, & Fortwendel J.R. (2024). MOB-mediated regulation of septation initiation network (SIN) signaling is required for echinocandin-induced hyperseptation in Aspergillus fumigatus. mSphere, 9(3), e00695-23.

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Tether Force Measurement Using Optical Tweezers

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To measure tether force, a membrane tether is pulled by a bead trapped with an optical tweezer (Tweez305, Aresis) equipped on the Ti2-A inverted microscope (Nikon). Membrane tubes were pulled to around 10 μm in length and then held until an apparent equilibrium force f was reached (Fig. S9). Then fluorescence images of the cell and the tether were taken for tether radius (r) measurements according to Eq. 5. Force on the bead was calculated from the displacement of the bead from the center of the trap and the trap stiffness (calibrated before each experiment by applying equipartition theorem to the thermal fluctuation of a trapped bead). Then membrane tension σ and bending stiffness

κ_{m}

were be calculated by^{43 (link)}:

σ = \frac{f}{2 π r}

κ_{m} = \frac{f r}{2 π}

Note that the pulling force f may contain contributions from the cytoskeleton and membrane asymmetry. Therefore, a more accurate measure of κ_m is to fit f/2π vs. r⁻¹ to a linear relation where the slope will report κ_m and the intercept will report the aforementioned additional contributions to tether pulling force (Fig. S9f).

Yang S., Miao X., Arnold S., Li B., Ly A.T., Wang H., Wang M., Guo X., Pathak M.M., Zhao W., Cox C.D, & Shi Z. (2022). Membrane curvature governs the distribution of Piezo1 in live cells. Nature Communications, 13, 7467.

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