Anti tyr 525

Manufactured by Bioworld Technology

Anti-Tyr-525 is a lab equipment product designed for protein analysis. It is a specific antibody that targets the Tyrosine-525 residue found in certain proteins. The core function of Anti-Tyr-525 is to facilitate the detection and quantification of proteins containing the Tyrosine-525 modification through immunoassay techniques.

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Lab products found in correlation

Anti tyr 416, by Cell Signaling Technology (2 mentions) Pan c src, by Proteintech (2 mentions) Pan syk, by Bioworld Technology (2 mentions) Anti tyr 1217, by Bioworld Technology (2 mentions) Pan plcγ2, by Bioworld Technology (2 mentions) Caspase 1, by Abcam (1 mentions) Il 1β, by Cell Signaling Technology (1 mentions) Pan akt, by Affinity Biosciences (1 mentions)

Close spelling variants of this product

Anti-Tyr (2 citations)

2 protocols using anti tyr 525

Platelet Function and Signaling Assays

Cited 1 time

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Platelet receptor expression, activation, aggregation and immunoblotting were studied as previously described.^{17 (link),18 (link)} Antibodies against c-Src (anti-Tyr-416, Cell Signaling Technology; pan-c Src, Proteintech), Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology) and PLCγ2 (anti-Tyr-1217 and pan-PLCγ2; Bioworld Technology), IL-1β (Cell Signaling Technology) and Caspase-1 (BioVision) were used.
Detailed methods of the electron microscopy of platelet spreading, and clot retraction are provided in the Online Supplement.

Qiao J., Wu X., Luo Q., Wei G., Xu M., Wu Y., Liu Y., Li X., Zi J., Ju W., Fu L., Chen C., Wu Q., Zhu S., Qi K., Li D., Li Z., Andrews R.K., Zeng L., Gardiner E.E, & Xu K. (2018). NLRP3 regulates platelet integrin αIIbβ3 outside-in signaling, hemostasis and arterial thrombosis. Haematologica, 103(9), 1568-1576.

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Quantification of Phosphorylated Signaling Proteins

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Levels of total and phosphorylated c-Src (anti-Tyr-416, Cell Signaling Technology; pan-c Src, Proteintech), Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology), PLCγ2 (anti-Tyr-1217 and pan-PLCγ2, Bioworld Technology), AKT (anti-Thr308 and anti-Ser473, Cell Signaling Technology; pan-AKT, Affinity Biosciences), or GSK-3β (anti-Ser9, Cell Signaling Technology; pan-GSK-3β, Affinity Biosciences) were assessed by SDS-PAGE/western blot. The density of protein band was quantified using Image J software and the phosphorylation level was shown as a ratio to the total level.

Wei G., Xu X., Tong H., Wang X., Chen Y., Ding Y., Zhang S., Ju W., Fu C., Li Z., Zeng L., Xu K, & Qiao J. (2020). Salidroside inhibits platelet function and thrombus formation through AKT/GSK3β signaling pathway. Aging (Albany NY), 12(9), 8151-8166.

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