The following antibodies were used for immunoblotting, immunofluorescence, or flow cytometry: SSRP1 (609702; Biolegend, San Diego, CA), SPT16 (607002; Biolegend), HRAS (sc-520; Santa Cruz, Dallas TX), p53 (ab27696, Abcam, Cambridge, United Kingdom), p21 (sc-6246; Santa Cruz), γH2AX (9718; Cell Signaling, Danvers, MA), RPA70 (2267; Cell Signaling), MCM7 (sc-9966; Santa Cruz), MCM4 (ab4459; Abcam), phospho-(Ser/Thr) ATM/ATR substrate antibody (2851; Cell Signaling), Chk1 (2360, Cell Signaling), p-Chk1-Ser317 (2344; Cell Signaling), p-Chk1-Ser296 (90178; Cell Signaling), cyclin A (4656; Cell Signaling), PCNA (2586; Cell Signaling), ssDNA (MAB3868; MilliporeSigma), Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11008; Thermo Fisher Scientific, Waltham, MA), and β-actin (A3854, Sigma-Aldrich). Where indicated, cells were treated with 1 or 2.5 mM HU (H8627; Sigma-Aldrich). Cell cycle analysis was done using propidium iodide or Hoechst 33342 stain. CBL0137 (1 μM; Incuron, Buffalo, NY, USA) was used as a positive control for p53 activation and p21 induction.
Spt16
Manufactured by BioLegend
Sourced in United Kingdom
The SPT16 is a laboratory centrifuge designed for general purpose applications. It features a maximum speed of 6,000 rpm and an adjustable timer for precise control of run times. The SPT16 accommodates various rotor options to accommodate different sample volumes and tube sizes.
Automatically generated - may contain errors
Lab products found in correlation
Phospho ser thr atm atr substrate antibody, by Cell Signaling Technology (1 mentions)
H ras, by Santa Cruz Biotechnology (1 mentions)
Cyclin a, by Cell Signaling Technology (1 mentions)
H8627, by Merck Group (1 mentions)
γh2ax, by Cell Signaling Technology (1 mentions)
P chk1 ser317, by Cell Signaling Technology (1 mentions)
Rpa70, by Cell Signaling Technology (1 mentions)
Ssrp1, by BioLegend (1 mentions)
P chk1 ser296, by Cell Signaling Technology (1 mentions)
Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Rna polymerase 2, by Santa Cruz Biotechnology (1 mentions)
Set1a, by Fortis Life Sciences (1 mentions)
2 protocols using spt16
1
Antibody Characterization for DNA Damage Response
2
Chromatin Immunoprecipitation Assay Panel
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ash2l (Bethyl, A300-489A), Cfp1 (Abcam ab198977), Dpy30 (Bethyl A304-296A), H3K4me1 (Abcam, ab8895), H3K4me2 (Abcam, ab7766), H3K4me3 (Abcam, ab8580), H3K9me2 (Abcam, ab1220), H3K79me2 (Abcam, ab3594), IgG (Millipore 12-370), Mll1 (Bethyl A300-086A), Mll2 (Santa Cruz, H300, sc-292359), Pax6 (Millipore, ab2237), Plekha1 (Novus, NBP1-86967), Rbbp5 (Bethyl, A300-109A), RNA polymerase II (Santa Cruz, N-20), Set1a (Bethyl, A300-289A), Snf2h (Active Motif, 39543), Spt16 (Biolegend, 607001), Ssrp (Biolegend 609702), TBP (Abcam, ab51841), vinculin (Abcam, ab129002), and Wdr5 (Bethyl A302-429A) were used.
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