Heidelberg spectralis device

Manufactured by Heidelberg Engineering

Sourced in Germany

The Heidelberg Spectralis is a diagnostic device that utilizes confocal scanning laser ophthalmoscopy and spectral-domain optical coherence tomography (SD-OCT) to produce high-resolution images of the retina and optic nerve. The device captures detailed cross-sectional views of the eye's structures, allowing for the assessment and monitoring of various eye conditions.

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Lab products found in correlation

Fluorescein sodium, by Alcon (1 mentions) Trg nw8, by Topcon (1 mentions) Spectralis hra oct, by Heidelberg Engineering (1 mentions)

8 protocols using heidelberg spectralis device

Retinal and Choroidal Imaging Using Advanced OCT Techniques

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Using complete protection by personal protective equipment (PPE), an experienced operator took images of the patients. To evaluate retinal total, inner, and outer thickness, the AngioVue (RTVue XR Avanti, Optovue, Fremont, CA, USA; software version 2018.0.0.14) system Raster and Retina Map protocols were used. Images with a signal strength of less than 45 were retaken. The Heidelberg Spectralis device (Heidelberg Engineering, Heidelberg, Germany) with enhanced depth imaging optical coherence tomography (EDI-OCT) was used to evaluate the choroid. Automatic Real-Time function (ART) was set 9–15, and the images with quality of less than 20 were retaken. Method of image acquisition has been previously reported [21 (link)]. To minimize the variation of CVI and choroidal thickness, all images were taken between 9 and 11 a.m. Patients were visited 1 month and 3 months after the first visit to repeat imaging.
Retinal parameters included retinal thickness in the whole image, fovea, parafovea, inner retina, and outer retina.

Abrishami M., Hassanpour K., Zamani G., Hosseini S.M., Shoeibi N., Emamverdian Z., Zamani A., Amini N, & Abrishami M. (2022). Longitudinal Alterations of Retinal and Choroidal Structure in Patients Recovered from COVID-19. Journal of Ophthalmology, 2022, 4123328.

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Longitudinal FCP and SD-OCT Assessment

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FCP testing was performed using the MAIA device (Centervue SpA, Padova, Italy) at baseline, week 6–8 and at month 7–8 post-treatment. The test pattern was a radial pattern with a central stimulus and 3 rings at 1°, 3°, and 5° eccentricity from the fovea with 12 stimuli each (total of 37 stimuli). Testing was conducted with a mesopic background (background luminance 1.27 cd/m²) and a 4–2 staircase strategy. At all 3 visits, SD-OCT B-scans were acquired with a Heidelberg Spectralis device (Heidelberg Engineering, Heidelberg, Germany; 20° × 20°, 49 B-scans).

Pfau M., van Dijk E.H., van Rijssen T.J., Schmitz-Valckenberg S., Holz F.G., Fleckenstein M, & Boon C.J. (2021). Estimation of current and post-treatment retinal function in chronic central serous chorioretinopathy using artificial intelligence. Scientific Reports, 11, 20446.

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High-resolution Spectral-domain OCT Imaging

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Patients underwent spectral-domain (SD)-OCT imaging using the Heidelberg Spectralis device (Heidelberg Engineering, Heidelberg, Germany) with high-resolution (HR), which may obtain OCT scans with an axial resolution of 7 μm in tissue, and a lateral resolution at the retinal surface estimated at approximately 5 μm, as well as an improved visualization of the choroid. Each set of SD-OCT scans consisted of 19 B-scans, each of which comprised 24 averaged scans, covering approximately a 5.5 × 4.5 mm area centered on the fovea. Furthermore, a single horizontal B-scan with HR and enhanced depth imaging (EDI) mode was obtained. To be included in the analysis, a signal strength of at least 25 was required (the manufacturer manual recommended 15 as the borderline quality score) [17 (link)].

Borrelli E., Zuccaro B., Zucchiatti I., Parravano M., Querques L., Costanzo E., Sacconi R., Prascina F., Scarinci F., Bandello F, & Querques G. (2019). Optical Coherence Tomography Parameters as Predictors of Treatment Response to Eplerenone in Central Serous Chorioretinopathy. Journal of Clinical Medicine, 8(9), 1271.

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Retinal OCT and OCTA Protocol

Cited 2 times

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Retinal spectral domain optical coherence tomography (OCT) and OCT angiography (OCTA) were performed using the Heidelberg Spectralis Device (Heidelberg Engineering, Heidelberg, Germany). For longitudinal assessment of the peripapillary retinal nerve fibre layer (pRNFL) thickness, a 3.4 mm ring scan was placed around the optic disc with the eye-tracking function (EBF) enabled for best accuracy as described [5 (link)]. The reported intraclass correlation coefficients (ICC) for repeated measurements are 1.00, which is excellent. Likewise, the EBF function was used for repeated measurements of volume scans, which were placed at the optic disc and macular. Automated segmentation was performed using the Heidelberg Spectralis Viewer Software. Pharmacological pupil dilation was not performed. All scans were quality-controlled using the OSCAR-IB criteria [6 (link)]. Reporting adhered to the nomenclature of the APOSTEL 2.0 guidelines [7 (link)].

Gandhewar R., Jurkute N, & Petzold A. (2022). The Role of the Inner Nuclear Layer for Perception of Persisting Tiling Inside a Monocular Scotoma. Brain Sciences, 12(11), 1542.

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Retinal Vasculature Visualization by FA

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FA was performed for the visualization of retinal vasculature. Mice were anesthetized at day 14 after treatment and placed on a custom-made platform positioned on the chin rest of Heidelberg Spectralis device (Heidelberg Engineering GmbH, Heidelberg, Germany). Hydroxypropylmethylcellulose (Methocel 2%; OmniVision, Neuhausen, Switzerland) was used to keep the cornea hydrated of each eye during imaging. 50 μL of fluorescein sodium (10%, Alcon, China) were injected intraperitoneally, then FA fundus images were acquired.

Tang Z., Ju Y., Dai X., Ni N., Liu Y., Zhang D., Gao H., Sun H., Zhang J, & Gu P. (2021). HO-1-mediated ferroptosis as a target for protection against retinal pigment epithelium degeneration. Redox Biology, 43, 101971.

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Multimodal Retinal Imaging Protocol

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All participants were subjected to a structured interview regarding their medical history, current health status, medication, and lifestyle. Prior to retinal imaging, participants’ pupils were dilated using tropicamide 1%. Retinal imaging consisted of stereoscopic 45° color fundus photographs centered on the macula (model TRG-NW8; Topcon, Tokyo, Japan) and blue fundus autofluorescence (FAF) images obtained by using the Heidelberg Spectralis device (Heidelberg Engineering, Heidelberg, Germany) with a standard 30° field of view (768 × 768 pixels). The automatic real time mode was used averaging 23 images to form a mean image. Spectralis HRA+OCT (Heidelberg Engineering, Heidelberg, Germany) was used with the automatic real time mode to obtain 20° × 20°, 49 SD-OCT B-scans centered on the fovea.

Martinez Villarruel Hinnerskov J., Krogh Nielsen M., Kai Thomsen A., Steffensen M.A., Honoré B., Vorum H., Nissen M.H, & Sørensen T.L. (2024). Chemokine Receptor Profile of T Cells and Progression Rate of Geographic Atrophy Secondary to Age-related Macular Degeneration. Investigative Ophthalmology & Visual Science, 65(1), 5.

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Multimodal Imaging Analysis of Macular PCV

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This was a post hoc analysis of 30 randomly selected patients, 15 from each of the 2 treatment arms, from the EVEREST II study (ClinicalTrials.gov identifier, NCT01846273), which was a prospective, multicenter, randomized clinical trial of patients with symptomatic macular PCV. The detailed design and results of the study were previously reported.⁸ (link) In brief, 322 patients with active macular PCV were equally randomized to receive combination therapy of verteporfin PDT and intravitreal ranibizumab (the combination therapy group) or intravitreal ranibizumab with sham PDT (the monotherapy group).
All patients underwent multimodal imaging using standardized imaging protocols at 5 mandatory time points: baseline and months 3, 6, 12, and 24. Fluorescein angiography (FA) and ICGA were performed using the Heidelberg Spectralis device (Heidelberg Engineering). Dynamic FA and ICGA were performed for the first 30 seconds, followed by stereo pair images of the study eye at 1, 3, and 5 minutes (FA and ICGA) as well as at 10 and 20 minutes for FA.

Tan C.S., Lim L.W, & Margaron P. (2021). Evolution of Polypoidal Lesions after Treatment of Polypoidal Choroidal Vasculopathy. Ophthalmology Science, 2(1), 100082.

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Multi-modal Imaging Assessment of Retina

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Figure 1 shows imaging and functional testing data for an exemplary patient. SD-OCT imaging of the macula was obtained with a Heidelberg Spectralis device (Heidelberg Engineering, Heidelberg, Germany) with a 20° × 20° (193 B-scans, HS mode, enhanced Automatic Real Time-Function was 16, covering an area of 6 mm × 6 mm. Shortwavelength FAF images (30° × 30°, HS mode, Automatic Real Time-Function 20) were performed using the same device.

Ansari G., Schärer N., Camenzind Zuche H., Gabrani C., Anders P., Pfau K., Valmaggia P., Giani A., Esmaeelpour M., Chingning Yamaguchi T., Prünte C.F., Maloca P.M., Schmetterer L., Scholl H.P.N, & Pfau M. (2023). The Optical Coherence Tomography and Microperimetry Biomarker Evaluation in Patients with Geographic Atrophy (OMEGA) Study: Design and Baseline Characteristics - OMEGA Report 1. Ophthalmic research, 66(1).

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