Hc pl apo cs 63 1.2 water immersion objective

Images were acquired using the Olympus IX 81 inverted laser scanning confocal microscope (Fluoview 500; Olympus Corporation, Tokyo, Japan) equipped with a 488-nm argon ion laser and a 60 × 1.0 numerical aperture PlanApo water immersion objective (Olympus). Green fluorescent protein was excited by 488-nm light and the emission was collected using a BA 505–525 filter. A BA 660 IF emission filter was used to observe chlorophyll autofluorescence. Confocal optical sections were obtained in 0.5- to l-μm increments. The images were color-coded green for GFP and magenta for chlorophyll autofluorescence.
The image presented in Figure 7 was made using a Leica SP8 laser-scanning microscope (Leica, Wetzlar, Germany) equipped with a solid-state laser with 488 nm light, HC PL APO CS 63×/1.2 water immersion objective (Leica, Wetzlar, Germany) and Leica Application Suite X software (LASX, Leica, Wetzlar, Germany). Images of GFP signal were acquired using the 488-nm laser line and emission was detected with a HyD (hybrid) detector in a range of 500–525 nm. For reflection microscopy, a 488-nm laser was used and light reflected into a band between 480 and 495 nm.

Image acquisition was done using a Leica SP8 laser scanning microscope (Leica, Wetzlar, Germany) equipped with a solid-state laser with 488 nm light, a HC PL APO CS 63×/1.2 water immersion objective (Leica) and Leica Application Suite X software (LASX, Leica). Images of GFP signal were acquired using the 488-nm laser light and the emission was detected with HyD (hybrid) detector in a range of 500–525 nm. Autofluorescence of the chloroplasts was detected in a range of 650–750 nm with a PMT detector.