Rabbit polyclonal anti cleaved caspase 3 asp175 antibody

Manufactured by Cell Signaling Technology

Rabbit polyclonal anti-cleaved caspase 3 (Asp175) antibody is a research-use antibody that specifically detects the cleaved form of caspase 3, a key executioner of apoptosis. This antibody is designed to be used in various immunoassay applications to study apoptosis-related processes.

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Lab products found in correlation

Peroxidase affinipure goat anti rabbit igg h l secondary antibody, by Jackson ImmunoResearch (1 mentions) Dmem f12 medium, by Corning (1 mentions) Horseradish peroxidase conjugated secondary igg antibodies, by Jackson ImmunoResearch (1 mentions) Rabbit anti gfp polyclonal antibody, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti ubiquitin antibody p4d1, by Santa Cruz Biotechnology (1 mentions) Fitc conjugated goat anti mouse secondary fab, by Jackson ImmunoResearch (1 mentions) Mouse monoclonal anti β tubulin, by Merck Group (1 mentions) Mouse monoclonal anti flag m2 antibody, by Merck Group (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti hemagglutinin ha antibody, by Fortrea (1 mentions) Trypsin edta, by Wisent (1 mentions) Penicillin streptomycin, by Wisent (1 mentions)

2 protocols using rabbit polyclonal anti cleaved caspase 3 asp175 antibody

Western Blotting of Sorted DC Lysates

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Western blotting of sorted DC lysates was performed according to a recently published protocol (13 (link)). The primary and secondary antibodies used in this assay were: Rabbit monoclonal anti-STING (D2P2F) (Cell Signaling Technology, Danvers, Massachusetts; Cat#13647), rabbit monoclonal anti-P-STING Ser³⁶⁵ (D8F4W) antibody (Cell Signaling Technology, Cat#72971), rabbit polyclonal anti-TBK1 antibody (Cell Signaling Technology, Cat#3013), rabbit monoclonal anti-TBK1 Ser¹⁷² (D52C2) antibody (Cell Signaling Technology, Cat#5483), rabbit monoclonal anti-IRF3 (D83B9) antibody (Cell Signaling Technology, Cat#4302), rabbit monoclonal anti-P-IRF3 Ser³⁹⁶ (4D4G) (Cell Signaling Technology, Cat#4947), rabbit monoclonal anti-NF-kappa-B p65 (C22B4) antibody (Cell Signaling Technology, Cat#4764), rabbit monoclonal anti-NF-kappa-B P-p65 Ser⁵³⁶ (93H1) antibody (Cell Signaling Technology, Cat#3033), rabbit polyclonal anti-cleaved caspase 3 (Asp175) antibody (Cell Signaling Technology, Cat#9661), mouse monoclonal anti-beta ACTIN, HRP (AC-15) (Abcam, Cambridge, Massachusetts; Cat#Ab49900) and Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody (Jackson ImmunoResearch Labs, West Grove, Pennsylvania, Cat#111-035-003).

Pang E.S., Daraj G., Balka K.R., De Nardo D., Macri C., Hochrein H., Masterman K.A., Tan P.S., Shoppee A., Magill Z., Jahan N., Bafit M., Zhan Y., Kile B.T., Lawlor K.E., Radford K.J., Wright M.D, & O’Keeffe M. (2022). Discordance in STING-Induced Activation and Cell Death Between Mouse and Human Dendritic Cell Populations. Frontiers in Immunology, 13, 794776.

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Antibody Sources for Western Blotting

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Mouse monoclonal anti-hemagglutinin (HA) antibody was purchased from Covance Inc. (Berkeley, CA); rabbit polyclonal anti-HA, monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and monoclonal anti-GFP antibodies were obtained from Abcam Inc. (Cambridge, MA); mouse monoclonal anti-Flag M2 antibody was from Sigma; rabbit polyclonal anti-GFP antibody was from Life Technologies. Mouse monoclonal anti-human transferrin antibody was from Invitrogen. Mouse monoclonal anti-β-tubulin was from Sigma. Mouse monoclonal anti-ubiquitin antibody (P4D1) was from Santa Cruz Biotechnology. Rabbit polyclonal anti-cleaved caspase-3 (Asp175) antibody was purchased from Cell Signaling Technology (kindly provided by Dr. Peter Siegel, McGill University). Horseradish peroxidase-conjugated secondary IgG antibodies, as well as FITC-conjugated goat anti-mouse secondary Fab were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). All Alexa Fluor® conjugated secondary antibodies were purchased from Molecular Probes (Eugene, OR). Alpha-minimum essential medium (α-MEM), fetal bovine serum, penicillin/streptomycin, and trypsin-EDTA were purchased from Wisent (Saint-Bruno, QC, Canada). The DMEM/F12 medium was from Corning. All other chemical and reagents were obtained from BioShop Canada (Burlington, ON, Canada), Sigma or Fisher Scientific and were of the highest grade available.

Ilie A., Gao A.Y., Reid J., Boucher A., McEwan C., Barrière H., Lukacs G.L., McKinney R.A, & Orlowski J. (2016). A Christianson syndrome-linked deletion mutation (∆287ES288) in SLC9A6 disrupts recycling endosomal function and elicits neurodegeneration and cell death. Molecular Neurodegeneration, 11(1), 63.

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