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Sr lab software

Manufactured by San Diego Instruments
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SR-LAB software is a data acquisition and analysis tool developed by San Diego Instruments. It is designed to interface with various laboratory instruments and sensors, allowing users to collect, visualize, and analyze data.

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Lab products found in correlation

Sr lab startle response system, by San Diego Instruments (2 mentions) Sr analysis, by San Diego Instruments (1 mentions) Freezeview software, by Harvard Apparatus (1 mentions) Modular test chamber, by Med Associates (1 mentions) Scopolamine hydrobromide trihydrate, by Merck Group (1 mentions) Sr lab startle chamber, by San Diego Instruments (1 mentions)

Close spelling variants of this product

SR-LAB test stations and software SR-LAB equipment and software SR-LAB

8 protocols using sr lab software

1

Measuring Acoustic Startle Response in Mice

Cited 1 time
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Acoustic startle response was tested using the SR-LAB Startle Response System with SR-LAB software (San Diego Instruments, San Diego, CA) as described previously (Yang et al., 2012 (link)). Test sessions began by placing the mouse in the Plexiglas holding cylinder for a 5-min acclimation period. For the next 8 min, mice were presented with each of six trial types across six discrete blocks of trials, for a total of 36 trials. The intertrial interval was 10–20 s. One trial type measured the response to no stimulus (baseline movement). The other five trial types measured startle responses to 40 ms sound bursts of 80, 90, 100, 110, or 120 dB. The six trial types were presented in pseudorandom order such that each trial type was presented once within a block of six trials. Startle amplitude was measured every 1 ms over a 65 ms period beginning at the onset of the startle stimulus. The maximum startle amplitude over this sampling period was taken as the dependent variable. A background noise level of 70 dB was maintained over the duration of the test session.
Shore A.N., Colombo S., Tobin W.F., Petri S., Cullen E.R., Dominguez S., Bostick C.D., Beaumont M.A., Williams D., Khodagholy D., Yang M., Lutz C.M., Peng Y., Gelinas J.N., Goldstein D.B., Boland M.J., Frankel W.N, & Weston M.C. (2020). Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy. Cell reports, 33(4), 108303.
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2

Prepulse Inhibition Assay in OGG1 Mice

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A prepulse inhibition test was performed to measure abnormalities in sensorimotor gating or startle response in OGG1 mice (~5 months old) [31 (link)]. The prepulse inhibition test was conducted using SR-LAB equipment and SR-LAB software from San Diego Instruments. Accelerometers were calibrated to 700  ±  5 mV and output voltages were amplified and analyzed for voltage changes using SR Analysis (San Diego Instruments, San Diego, CA, USA). Each mouse was tested for 30 min. The background white noise was maintained at 65 dB, and each mouse was subjected to 80 randomized trials of pulse alone (100 dB above background), prepulse alone (4, 8 or 16 dB above background), prepulse plus pulse and no pulse, with five pulse-alone trials performed at the start and end of the 80 trials. Time intervals between trials were randomized from 5 s to 20 s, with a delay of 100 ms between the prepulse and the pulse. Prepulse inhibition was thus measured as a decrease in the amplitude of startle response to a 100 dB (above background) acoustic startle pulse following each prepulse (4, 8 and 16 dB above background).
Bhatia S., Bodenstein D., Cheng A.P, & Wells P.G. (2023). Altered Epigenetic Marks and Gene Expression in Fetal Brain, and Postnatal Behavioural Disorders, Following Prenatal Exposure of Ogg1 Knockout Mice to Saline or Ethanol. Cells, 12(18), 2308.
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3

Auditory Fear Conditioning in Mice

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Auditory fear conditioning was carried out as previously described (Dias and Ressler, 2014 (link)). Briefly, mice were pre-exposed to sound attenuated conditioning chambers (San Diego Instruments) (grid floors, room light on, cleaned with Quatricide: Context A) for 3 consecutive days before training. On the day of auditory fear conditioning in Context A, mice received five CS−US pairings (CS: 30 second, 6 kHz, 75 db tone) (US: 500 ms, 0.6mA foot-shock) wherein the tone co-terminated with the mild foot-shock with a 5 minute inter-trial interval (ITI). Where an unpaired condition was used, the same CS and US parameters were used with no co-termination and presented in a random sequence. The percentage of time spent freezing during fear acquisition was measured by SR-LAB software (San Diego Instruments). The consolidation of fear memory was tested 24 hours after fear conditioning in a novel context (modular test chambers; Med Associates Inc.) (plexiglass floor, room light off, red chamber lights on, cleaned with EtOH: Context B) when mice were exposed to 5 CS tones with a 2 minute ITI. Freezing during the tone presentations was measured with FreezeView software (Coulbourn Instruments). All statistical analyses were conducted using a repeated-measure ANOVA design with Bonferroni correction.
Dias B.G., Goodman J.V., Ahluwalia R., Easton A.E., Andero R, & Ressler K.J. (2014). Amygdala-dependent fear memory consolidation via miR-34a and Notch signaling. Neuron, 83(4), 906-918.
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4

Startle Response and Prepulse Inhibition Assay

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PPI was measured using automated startle chambers (San Diego Instruments, San Diego, CA, USA), as described previously [37 (link)]. In brief, rats were placed in a transparent Plexiglas cylinder that sat within a sound-attenuating cabinet. Audiogenic stimuli were delivered with a speaker, and whole-body startle responses were measured using the automated SR-Lab software (San Diego Instruments). The 30-minute session consisted of 80 trials presented at variable intervals (8–27 s), 8 no tone (i.e., only background, 70 dB), 32 pulse-alone (4 blocks of 8 × 115 dB) trials, and 40 prepulse trials (8 of each: 2, 4, 8, 12, or 16 dB above background followed 100 ms later by the 115 dB pulse). Startle data were measured using all four blocks of pulse-alone trials. The percentage of PPI was calculated as the difference in amplitude between the startle response to the pulse-alone trials and the prepulse-pulse trials, divided by the response to the pulse-alone trial × 100%.
Saline or 0.3 mg/kg scopolamine (Scopolamine hydrobromide trihydrate; Sigma-Aldrich, St. Louis, MO, USA) was administered subcutaneously 30 min prior to PPI testing. Scopolamine was dissolved in saline to a dose based on previous literature [25 (link),38 (link)] and administered in a volume of 1 ml/kg. Using a randomized, crossover protocol, rats received both treatments with 3–4 days allowed between each experiment.
Ch’ng S.S., Walker A.J., McCarthy M., Le T.K., Thomas N., Gibbons A., Udawela M., Kusljic S., Dean B, & Gogos A. (2020). The Impact of Removal of Ovarian Hormones on Cholinergic Muscarinic Receptors: Examining Prepulse Inhibition and Receptor Binding. Brain Sciences, 10(2), 106.
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5

Measuring Acoustic Startle Response in Mice

Cited 1 time
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Acoustic startle response was tested using the SR-LAB Startle Response System with SR-LAB software (San Diego Instruments, San Diego, CA) as described previously (Yang et al., 2012 (link)). Test sessions began by placing the mouse in the Plexiglas holding cylinder for a 5-min acclimation period. For the next 8 min, mice were presented with each of six trial types across six discrete blocks of trials, for a total of 36 trials. The intertrial interval was 10–20 s. One trial type measured the response to no stimulus (baseline movement). The other five trial types measured startle responses to 40 ms sound bursts of 80, 90, 100, 110, or 120 dB. The six trial types were presented in pseudorandom order such that each trial type was presented once within a block of six trials. Startle amplitude was measured every 1 ms over a 65 ms period beginning at the onset of the startle stimulus. The maximum startle amplitude over this sampling period was taken as the dependent variable. A background noise level of 70 dB was maintained over the duration of the test session.
Shore A.N., Colombo S., Tobin W.F., Petri S., Cullen E.R., Dominguez S., Bostick C.D., Beaumont M.A., Williams D., Khodagholy D., Yang M., Lutz C.M., Peng Y., Gelinas J.N., Goldstein D.B., Boland M.J., Frankel W.N, & Weston M.C. (2020). Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy. Cell reports, 33(4), 108303.
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6

Startle Response Conditioning in SR-LAB Chambers

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All experiments were conducted in eight, identical SR-LAB startle chambers with cylindrical animal enclosures (San Diego Instruments, San Diego, CA). A high-frequency loudspeaker, mounted 24 cm above the enclosures, provides background noise as well as the startle eliciting white-noise bursts (WNB). During the FPS, a single LED bulb positioned on the ceiling inside the startle chamber was used as the visual conditioned stimulus (CS). In addition, a grid floor made of stainless steel bars placed inside the enclosures was used to deliver foot shocks as the unconditioned stimulus (US). The presentation and sequence of all stimuli as well as recording of the responses were automatically controlled by the SR-LAB software (San Diego Instruments).
Moaddab M, & Dabrowska J. (2017). Oxytocin receptor neurotransmission in the dorsolateral bed nucleus of the stria terminalis facilitates the acquisition of cued fear in the fear-potentiated startle paradigm in rats. Neuropharmacology, 121, 130-139.
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7

Acoustic Startle Response in Rats

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Rats were habituated once to the procedure. They received mock IP injections and were subsequently placed in ASR boxes for 5 min two days prior to the start of the experiment. The test was done over three experimental days where overnight fasted rats received IP-injections of vehicle, OT, or OT-B12 in a counterbalanced design. Two days were allowed between each experimental day. On each testing day, rats were left for 30 min of acclimatization in the testing room before the drug injections. 30 min after injection, rats were placed in a plexiglas cylinder connected to a piezoelectric accelerometer permitting recording of the amplitude of startle responses. Cylinders were connected to and put into ventilated chambers with the light on, background noise of 50 dB, and acoustic stimuli of 90 or 120 dB (each 50 ms long). After 5 min of habituation to the chambers, the acoustic stimuli were delivered in a randomized order (20 repetitions of 90dB and 10 repetitions of 120dB) with inter-stimulus pauses fluctuating between 20 and 40 seconds using the SR Lab Software (San Diego Instruments, San Diego, USA). The mean of peak amplitude response to each acoustic stimulus (in millivolts) across all repetitions was calculated. The cylinders were cleaned with 70% ethanol and water after each test.
Asker M., Krieger J., Liles A., Tinsley I.C., Borner T., Maric I., Doebley S., Furst C.D., Börchers S., Longo F., Bhat Y.R., De Jonghe B.C., Hayes M.R., Doyle R.P, & Skibicka K.P. (2023). Peripherally restricted oxytocin is sufficient to reduce food intake and motivation, while CNS entry is required for locomotor and taste avoidance effects. Diabetes, Obesity & Metabolism, 25(3), 856-877.
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8

Acoustic Startle Response in Rats

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Rats were habituated once to the procedure. They received mock IP injections and were subsequently placed in acoustic startle response (ASR) boxes for 5 min 2 days before the start of the experiment. The test was done over three experimental days where overnight fasted rats received IP injections of vehicle, OT or OT‐B12 in a counterbalanced design. Two days were allowed between each experimental day. On each testing day, rats were left for 30 min of acclimatization in the testing room before the drug injections. Thirty minutes after injection, rats were placed in a plexiglass cylinder connected to a piezoelectric accelerometer permitting recording of the amplitude of startle responses. Cylinders were connected to and put into ventilated chambers with the light on, background noise of 50 dB, and acoustic stimuli of 90 or 120 dB (each 50 ms long). After 5 min of habituation to the chambers, the acoustic stimuli were delivered in a randomized order (20 repetitions of 90 dB and 10 repetitions of 120 dB) with inter‐stimulus pauses fluctuating between 20 and 40 s using the SR Lab Software (San Diego Instruments, San Diego, CA, USA). The mean of peak amplitude response to each acoustic stimulus (in millivolts) across all repetitions was calculated. The cylinders were cleaned with 70% ethanol and water after each test.
Asker M., Krieger J., Liles A., Tinsley I.C., Borner T., Maric I., Doebley S., Furst C.D., Börchers S., Longo F., Bhat Y.R., De Jonghe B.C., Hayes M.R., Doyle R.P, & Skibicka K.P. (2023). Peripherally restricted oxytocin is sufficient to reduce food intake and motivation, while CNS entry is required for locomotor and taste avoidance effects. Diabetes, Obesity & Metabolism, 25(3), 856-877.
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