High performance liquid chromatography grade organic solvents

Manufactured by Merck Group

Sourced in Germany

High performance liquid chromatography (HPLC)-grade organic solvents are pure, high-quality solvents designed for use in HPLC systems. They are formulated to meet the stringent purity requirements of HPLC applications, ensuring reliable and consistent performance in analytical and preparative separations.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Sf 900 3 sfm insect culture medium, by Thermo Fisher Scientific (3 mentions) Reduced nicotinamide adenine dinucleotide phosphate nadph, by Merck Group (2 mentions) Bac to bac baculovirus expression system, by Thermo Fisher Scientific (2 mentions) Liquid chromatography mass spectrometry grade acetonitrile, by Merck Group (2 mentions) Primestar hs dna polymerase, by Takara Bio (2 mentions) Cellfectin 2 reagent, by Thermo Fisher Scientific (2 mentions) Procaine, by Merck Group (1 mentions) Milli q a10 system, by Merck Group (1 mentions) Pfastbac dual vector, by Thermo Fisher Scientific (1 mentions) Lc ms grade acetonitrile, by Merck Group (1 mentions) Cyclosporin a, by Maclin Biochemical Technology (1 mentions) Carbamazepine, by LGC (1 mentions) Restriction enzyme, by Takara Bio (1 mentions) Dna ligation kit, by Takara Bio (1 mentions) Sc 53850, by Santa Cruz Biotechnology (1 mentions) Tolbutamide, by LGC (1 mentions) Losartan, by LGC (1 mentions) Losartan carboxylic acid, by LGC (1 mentions) Midazolam, by LGC (1 mentions)

4 protocols using high performance liquid chromatography grade organic solvents

In Vitro Metabolite Formation Assay

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Lidocaine was obtained from Shanghai Canspec Scientific Instruments Co., Ltd (Perfemiker, Shanghai, China) and its metabolite MEGX was purchased from Toronto Research Chemicals Inc (Toronto, Ontario, Canada). The reduced nicotinamide adenine dinucleotide phosphate (NADPH) and procaine (internal standard) were obtained from Sigma (St Louis, MO, USA). Cytochrome b5 microsomes were kind gifts from Beijing Hospital.24 (link)
Spodoptera frugiperda 21 (Sf21) insect cells, fetal bovine serum, Sf-900™ III SFM insect culture medium, pFastBac Dual vector and Bac-to-Bac Baculovirus Expression System were purchased from Invitrogen (Carlsbad, CA, USA). The CORTECS^® ultra-performance liquid chromatography (UPLC)^® hydrophilic interaction liquid chromatography (HILIC)^® column (2.1×50 mm, 1.6 µM) was obtained from the Waters (Dublin, Ireland). High performance liquid chromatography (HPLC)-grade organic solvents and LC-MS grade acetonitrile were from Merck (Darmstadt, Germany). Ultrapure water was freshly purified by a Milli-Q A10 System (Milli-pore, Billerica, MA, USA). Formic acid (FA, 98% purity) of LC-MS grade was from Sigma-Aldrich (Munich, Germany). All the other chemicals and solvents used were of the analytical grade commercially available.

Fang P., Tang P.F., Xu R.A., Zheng X., Wen J., Bao S.S., Cai J.P, & Hu G.X. (2017). Functional assessment of CYP3A4 allelic variants on lidocaine metabolism in vitro. Drug Design, Development and Therapy, 11, 3503-3510.

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In Vitro CYP3A4 Metabolism Assay

Cited 1 time

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Cyclosporin A (CsA) was purchased from Shanghai Macklin Biochemical Co., Ltd. Its metabolite CsA M-17 (AM-1) was purchased from Toronto Research Chemicals Inc. Diazepam (internal standard, IS) was purchased from Shanghai Hanxiang Biological Technology Co., Ltd. Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Sigma-Aldrich Company (St Louis, MO). High-performance liquid chromatography (HPLC)-grade organic solvents were obtained from Merck (Darmstadt, Germany). Recombinant human CYP3A4 and cytochrome b5 were prepared by our group as described previously (Zhou et al., 2019 (link)). The rat liver microsomes (RLMs) and human liver microsomes (HLMs) were from Corning Life Sciences Co., Ltd. The information on 132 drugs is presented in Supplementary Table S1.

Kong Q., Gao N., Wang Y., Hu G., Qian J, & Chen B. (2023). Functional evaluation of cyclosporine metabolism by CYP3A4 variants and potential drug interactions. Frontiers in Pharmacology, 13, 1044817.

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Baculoviral Expression of CYP3A4 and OR

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Quinine, 3-hydroxyquinine (3-OH quinine), and carbamazepine (internal standard [IS]) were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Cells from the fall armyworm (Spodoptera frugiperda [Sf]21), Sf-900™ III SFM insect culture medium, fetal bovine serum, Cellfectin^® II Reagent, and the Bac-to-Bac™ baculovirus cell expression system were obtained from Invitrogen (Carlsbad, CA, USA). PrimeSTAR^® HS DNA polymerase, restriction enzymes, and a DNA ligation kit were purchased from TaKaRa Bio (Shiga, Japan). A human cDNA clone for oxidoreductase (OR) (catalog number SC100401) and a wild-type CYP3A4 cDNA clone (catalog number SC125488) were obtained from Origene (Rockville, MD, USA). Mouse monoclonal anti-OR antibody, anti-CYP3A4 antibody, and anti-CYP3 antibody were from Santa Cruz Biotechnology (sc-25270, sc-53850, and sc-365415; Santa Cruz, CA, USA). Rabbit monoclonal anti-CYP3A4 antibody was purchased from Abcam (ab 3572; Cambridge, UK). A regenerating system for the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Promega (Madison, WI, USA). High-performance liquid chromatography-grade organic solvents and liquid chromatography–mass spectrometry-grade acetonitrile were purchased from Merck (Darmstadt, Germany). All other chemicals and reagents used were of the highest purity available.

Zhou X.Y., Hu X.X., Wang C.C., Lu X.R., Chen Z., Liu Q., Hu G.X, & Cai J.P. (2019). Enzymatic Activities of CYP3A4 Allelic Variants on Quinine 3-Hydroxylation In Vitro. Frontiers in Pharmacology, 10, 591.

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Baculovirus Expression and Drug Metabolism

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TaKaRa MiniBEST Whole Blood Genomic DNA Extraction Kit, PrimeSTAR HS DNA polymerase, restriction enzymes, and T4 DNA ligase were purchased from Takara Bio Inc. (Kusatsu, Japan). Spodoptera frugiperda (Sf) 21 cells, fetal bovine serum, Sf-900™ III SFM insect culture medium, Cellfectin II Reagent, and the Bac-to-Bac™ Baculovirus Expression System were purchased from Invitrogen (Carlsbad, CA, United States). Tolbutamide, 4-hydroxy Tolbutamide, losartan, losartan carboxylic acid, warfarin, 7-hydroxy warfarin, diazepam and midazolam (internal standard [IS]) were purchased from Toronto Research Chemicals (Toronto, Canada). Rabbit polyclonal anti-CYP2C9 antibody and mouse monoclonal anti-OR antibody were obtained from Abcam (Cambridge, United Kingdom) and Santa Cruz Biotechnology (Santa Cruz, CA, United States), respectively. A SuperSignal West Pico Trial Kit was obtained from Thermo Scientific (Rockford, IL, United States). A regeneration system for the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Promega (Madison, WI, United States). High-performance liquid chromatography-grade organic solvents and liquid chromatography–mass spectrometry-grade acetonitrile were purchased from Merck (Darmstadt, Germany). All other chemicals and reagents used were of the highest purity available.

Zhou X.Y., Lu X.R., Li Y.H., Ma Y.Q., Zhao S.W., Wang F., Xu R.A., Hu G.X, & Cai J.P. (2021). Identification and Enzymatic Activity Evaluation of a Novel CYP2C9 Allelic Variant Discovered in a Patient. Frontiers in Pharmacology, 12, 619339.

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