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Dc250

Manufactured by Leica
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Sourced in Switzerland

The Leica DC250 is a digital camera system designed for microscopy applications. It features a high-resolution sensor and advanced image processing capabilities to capture detailed micrographs. The core function of the Leica DC250 is to provide users with a reliable and efficient tool for capturing and analyzing microscopic samples.

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Lab products found in correlation

Formalin, by Merck Group (2 mentions) Alexa488 conjugated donkey anti goat, by Thermo Fisher Scientific (2 mentions) Anti rabbit igg h l antibodies, by Thermo Fisher Scientific (2 mentions) Dapi staining solution, by Merck Group (2 mentions) Mz10f, by Leica (1 mentions) Dmlb fluorescence microscope, by Leica (1 mentions) Plan apo 63, by Leica (1 mentions) Cw4000 karyo software, by Leica (1 mentions)

5 protocols using dc250

1

Visualizing Sperm Ejaculation Dynamics

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The location of sperm ejection was monitored using a MZ10F stereomicroscope equipped with filters for UV light and Green Fluorescent Protein (GFP) detection (Leica Microsystems Ltd; Germany). Images were taken using a Leica DC250 camera and the Leica Application suite software.
Duménil C., Woud D., Pinto F., Alkema J.T., Jansen I., Van Der Geest A.M., Roessingh S, & Billeter J.C. (2016). Pheromonal Cues Deposited by Mated Females Convey Social Information about Egg-Laying Sites in Drosophila Melanogaster. Journal of Chemical Ecology, 42, 259-269.
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2

Fluorescence Microscopy Imaging Protocol

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Chromosomes were viewed and photographed with a Leica (Heerbrugg, Switzerland) DMLB fluorescence microscope equipped with a computer-assisted Leica DC 250 digital camera system. Red, green, and blue images were captured in black and white using appropriate filters for TRITC, FITC, and DAPI excitation, respectively. Digital images were combined, and then the color balance, brightness, and contrast were processed uniformly across the image.
Ortiz A.M., Chalup L., Silvestri M.C., Seijo G, & Lavia G.I. (2023). Genomic relationships of the polyploid rhizoma peanut (Arachis glabrata Benth.) inferred by genomic in situ hybridization (GISH). Anais da Academia Brasileira de Ciencias, 95(2).
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3

Immunofluorescent Detection of Vpr in Daudi Cells

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Daudi cells were adsorbed on polyLysine-treated slides and fixed with Formalin (Sigma) containing 1% Triton-X-100. Cells were labeled with anti-Vpr antibody followed by labeling with a mixture of Alexa488-conjugated donkey anti-goat and anti-rabbit IgG (H + L) antibodies (Life Technologies). Nuclei were stained with DAPI staining solution from Sigma Aldrich. Cells were mounted on glass slides covered with anti-fade medium (Hardset Vectashield). Two-color images were obtained with a light microscope, Leica DC250 (Leica) with a Plan Apo 63 ×/1.32–0.6 oil-immersion objective lens. Digital images were processed with the ImageJ software (NIH Image). Percentages of Vpr+ Daudi B-cells were determined by using the Cell Counting plugin of ImageJ. A minimum of 750 cells were counted per 6-well plate.
Eldin P., Péron S., Galashevskaya A., Denis-Lagache N., Cogné M., Slupphaug G, & Briant L. (2020). Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination. Journal of Translational Medicine, 18, 310.
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4

Daudi B-Cell Vpr Localization Analysis

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Daudi cells were adsorbed on polyLysine-treated slides and xed with Formalin (Sigma) containing 1% Triton-X-100. Cells were labeled with anti-Vpr antibody followed by labeling with a mixture of Alexa488conjugated donkey anti-goat and anti-rabbit IgG (H+L) antibodies (Life Technologies). Nuclei were stained with DAPI staining solution from Sigma Aldrich. Cells were mounted on glass slides covered with anti-fade medium (Hardset Vectashield). Two-color images were obtained with a light microscope, Leica DC250 (Leica) with a Plan Apo 63 × /1.32-0.6 oil-immersion objective lens. Digital images were processed with the ImageJ software (NIH Image). Percentages of Vpr + Daudi B-cells were determined by using the Cell Counting plugin of ImageJ. A minimum of 750 cells were counted per 6-well plate.
Eldin P., Péron S., Galashevskaya A., Denis-Lagache N., Cogné M., Slupphaug G., & Briant L. (2020). Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination.
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5

Chromosome Metaphase Analysis Protocol

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Cells were cultured in medium containing Colcemid (0.1 µg/ml) for 3 h, incubated for 30 min in hypotonic solution, fixed, and stained using Giemsa (Kario MAX Giemsa). Metaphases were examined under a Leica HC microscope equipped with a digital camera Leica DC250. Images were analyzed using Leica CW4000 Karyo software.
Pennarossa G., Manzoni E.F.M., Ledda S., deEguileor M., Gandolfi F, & Brevini T.A.L. (2019). Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts. Stem cell reviews and reports, 15(1).
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