All experiments were conducted in accordance with the Association for Research in Vision and Ophthalmology Statement on the Use of Animals in Ophthalmic and Vision and approved by the national investigation office in Koblenz, Germany (23 177-07/G 15-1-053). Female Sprague-Dawley rats (200 g) were obtained from Charles River (Sulzfeld, Germany) and housed in climate-controlled rooms with 12 h light-dark cycle and fed ad libitum. Anesthesia was induced by intramuscular injection of 0.185 ml/kg body weight medetomidine hydrochloride (1 mg/ml Dorbene vet., Pfizer, New York, NY) and reversed using atipamezol hydrochloride (5 mg/ml Alzane, Zoetis Österreich GmbH, Wien, Austria). Animals were anesthetized for surgery, intravitreal injection and optical coherence tomography (OCT). Episcleral vein occlusion (EVO) by cauterization was performed unilaterally on the left eye, the right fellow eye served as the control.
Dorbene vet
Manufactured by Pfizer
Sourced in United States
Dorbene Vet is a veterinary lab equipment product designed for use in animal healthcare settings. It is a compact, multi-functional device that can perform a variety of laboratory tests and analyses. The core function of Dorbene Vet is to provide accurate and reliable diagnostic data to support veterinary professionals in their clinical decision-making.
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5 protocols using dorbene vet
1
Episcleral Vein Occlusion in Sprague-Dawley Rats
2
Sprague-Dawley Rat Glaucoma Model
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Female Sprague–Dawley rats (n = 12; 250–300 g) at the same age were used for glaucoma animal model in this study. The use of animals for research purposes was approved by the Health Investigation Office Rhineland-Palatinate (permission number: 14-1-085; approvals date: 13 October 2014). All experimental procedures were in accordance with the Association of Research in Vision and Ophthalmology (ARVO)—Statement for the Use of Animals in Ophthalmic and Vision Research, and the guidelines of the Institutional Animal Care and Use Committee. Animals were kept under a day- and night-cycle of 12 h at the Translational Animal Research Center (TARC) of the University Medical Center of Johannes-Gutenberg University Mainz.
Minimizing the discomfort and pain for the animals was prioritized in the experimental interventions. A mixture of medetomidine hydrochlorid (Dorbene vet., Pfizer, New York, NY, USA) and ketamine (Inresa Arzneimittel, Freiburg, Germany) was administered intraperitoneally for anesthesia, and oxybuprocain (Novesine, OmniVision, Puchheim, Germany) was applied topically to ocular surfaces. To reduce post operation pain, novaminsulfon (Novalgin, Ratiopharm, Ulm, Germany) was injected subcutaneously after intervention. Health condition and general behavior of the animals were observed daily by TARC staff.
Minimizing the discomfort and pain for the animals was prioritized in the experimental interventions. A mixture of medetomidine hydrochlorid (Dorbene vet., Pfizer, New York, NY, USA) and ketamine (Inresa Arzneimittel, Freiburg, Germany) was administered intraperitoneally for anesthesia, and oxybuprocain (Novesine, OmniVision, Puchheim, Germany) was applied topically to ocular surfaces. To reduce post operation pain, novaminsulfon (Novalgin, Ratiopharm, Ulm, Germany) was injected subcutaneously after intervention. Health condition and general behavior of the animals were observed daily by TARC staff.
3
Quantitative Neuroanatomy by Immunofluorescence
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Mice were anesthetized with a mixture of ketamine (75 mg/kg, i.p.; Imalgene 1000, Merial, United States) and medetomidine (1 mg/kg, i.p.; Dorbene Vet, Pfizer, United States), and transcardially perfused with 0.9% saline. Brains were carefully removed, fixed overnight with 4% paraformaldehyde and immunofluorescence experiments were performed in cryostat coronal brain sections (20 μm thick). Sections were incubated overnight with the primary antibodies: rabbit anti-S100β (1:200, DakoCytomation; AB_2315306) and rabbit anti-NeuN (1:100, Cell Signaling Technology; AB_2651140). In the next day, incubation with the secondary antibody Alexa Fluor® 594 donkey anti-rabbit (1:1000, Thermo Fisher Scientific; AB_2556543) was carried out. Images were acquired in an Olympus Fluoview FV1000 confocal microscope (Olympus, Hamburg, Germany), and the number of S100β+ and NeuN+ cells was calculated using the ImageJ plugin – “Cell counter1 .”
4
Perioperative Antimicrobial and Pain Management Protocol
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Spiramycin and metronidazole (Buccoval ® , Sogeval) were administered per os (oral administration) and penicillin procaine and benzathine (Duplocilline ® , Intervet S.A) were injected subcutaneously for antimicrobial prophylaxis the day before surgery. At the day of the surgery, the dogs were weighed and anesthetized. After an intramuscular injection of medetomidine (Dorbene Vet ® , Pfizer), anesthesia was induced by intravenous administration of ketamine (Ketamine 1000 ® , Virbac) followed by inhalation of an O 2isoflurane mixture (Isoflo ® , Axience). A pre-operative subcutaneous injection of carprofene (Rimadyl ® , Pfizer) and buprenorphine (Buprecare ® , Axience) was administered. The mandibles were disinfected with application of 0.2% chlorhexidine (Cooper) and the surgical region was locally anesthetized. Postoperatively, Spiramycin and metronidazole (per os, 75 000 IU/kg of spiramycine and 12,5 mg/kg of metronidazole, Buccoval ® , Sogeval) were administered daily, for at least 14 days for antimicrobial prophylaxis. Carprofene (per os, 4 mg/kg Carprodyl ® , CEVA) was administered daily for at least 5 days as anti-inflammatory drug. Postoperative pain was controlled with Buprenorphine (SC, 0.01 mg/kg, Buprecare®, Axience), it was injected when needed. For local disinfection, Chlorhexidine (0.2 %, Cooper) was applied on the sites once a day until wound healing was complete.
5
Dental Surgery Protocol in Dogs
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The dogs were fasted for food 12 to 24 hours before the surgeries. Spiramycin and metronidazole (Buccoval®, Sogeval) were administered per os and penicilline, procaine and benzathine (Duplocilline®, Intervet S.A) were injected subcutaneously the day before surgery. After an intramuscular injection of medetomidine (Dorbene Vet®, Pfizer), anesthesia was performed by intravenous administration of ketamine (Ketamine 1000®, Virbac) and atropine (Atropinum Sulfuricum, Aguettant) followed by inhalation of an O2isoflurane mixture (Isoflo®, Axience). A pre-operative subcutaneous injection of carprofene (Rimadyl®, Pfizer) and buprenorphine (Buprecare®, Axience SAS) was additionally administered. An intravenous infusion with an electrolyte solution was performed during surgery (Ringer lactate, CEVA Santé Animal). The dogs were placed on a heating pad and the mandibles disinfected by application of a 0.2% chlorexhidine solution (Cooper). Prior to the beginning of the surgeries, injections of 2% lidocaïne with adrenaline (Lidocaïne adrenaline®, Aguettant) were administered for each hemimandible. An overview on the schedule of the study is displayed in Fig. 1.
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